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Microbial agent and application thereof to passivation of heavy metals in soil

A technology of microbial agents and heavy metals, applied in the field of environmental restoration of microbial agents, can solve the problems of high initial cost and achieve the effects of short application period, easy promotion and application, simple and economical preparation and use methods

Inactive Publication Date: 2019-01-04
ANSTEEL GRP MINING CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This invention can effectively restore soil polluted by heavy metals, and can improve soil fertilizer efficiency without introducing new pollutants, but it needs to be fermented and produced, and the initial cost is high

Method used

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  • Microbial agent and application thereof to passivation of heavy metals in soil
  • Microbial agent and application thereof to passivation of heavy metals in soil
  • Microbial agent and application thereof to passivation of heavy metals in soil

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Embodiment

[0038] Activation medium preparation: the medium components used are peptone 5.0g / L, yeast extract powder 2.0g / L, glucose 20.0g / L, dipotassium hydrogen phosphate 1.0g / L, magnesium sulfate 0.5g / L, pH=6.5; Propagation medium preparation: the medium components used are potato extract powder 6.0g / L, glucose 20.0g / L, agar 20.0g / L, pH=5.6.

[0039] The above-mentioned medium was used at 30 °C, 180 r·min -1 Cultured on a shaker for 2 days, and the fungi to be used were activated; the activated test strains were inoculated into the conical flasks that had been sterilized by high temperature and added to the culture medium for expansion, at 30 °C, 220 r·min -1 Shake on a shaker for 24 hours, and dilute the fungi with sterile water to a concentration of 1 × 10 effective bacteria. 7 CFU / ml, counted by a hemocytometer, and the three fungal microbial cultures of Mortierella, Trichoderma aculeatus and Mucor curcumae after activation and propagation were mixed evenly in equal volumes and ap...

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Abstract

The invention relates to a microbial agent. The microbial agent is formed by three fungi including mortierella, trichoderma asperellum and mucor circinelloides. The fungi are activated, propagated anddiluted into 1-9*10<7>CFU / ml fungal liquid respectively, and the three types of fungal liquid are subjected to isovolumetric mixing to obtain the microbial agent. The application of the microbial agent to passivation of heavy metals in soil refers to a method of plant infection with the microbial agent to form infected okra, alfalfa and soybean plants, and removal rates of copper, zinc, lead, cadmium and manganese is 75.69%, 57.60%, 89.61%, 82.83% and 68.77% respectively. Preparation and application methods of the microbial agent are simple and economical, soil physiochemical properties can be effectively improved, fertilizer and water retention capacity of soil is increased, secondary pollution is avoided, and a short application period is realized.

Description

technical field [0001] The invention relates to the field of environmental remediation utilizing microbial inoculants, in particular to a microbial inoculant and its application of passivating heavy metals in soil. Background technique [0002] Heavy metal pollution is the main soil environmental pollution problem in my country. About 150 million mu of cultivated land is polluted by heavy metals such as cadmium, arsenic, mercury, copper, and zinc in my country, and more than 10 million tons of grains are polluted by heavy metals such as "cadmium rice" and "arsenic rice" every year, resulting in direct economic losses. More than 20 billion yuan. [0003] All nutrients necessary for plants in the process of growth and development come from the soil, and the harm of heavy metals to plants also enters plants through the soil. The distribution and morphology of heavy metals in soil determine the possible migration and transformation behavior, potential toxicity and bioavailabili...

Claims

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Application Information

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IPC IPC(8): C12N1/14B09C1/10C12R1/645C12R1/885C12R1/785
CPCB09C1/105C12N1/14
Inventor 邵安林许灏李超亮徐连生宋英辉李鑫鑫
Owner ANSTEEL GRP MINING CO LTD
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