253results about How to "Reduced ability to migrate" patented technology

The invention belongs to the technical field of gene engineering, and discloses sgRNA of targeting IGF-IR gene. The nucleotide sequence of the sgRNA of targeting IGF-IR gene is represented by SEQ ID NO:2. According to applications, a Crispr/cas9-sgRNA lentiviral vector system is adopted, knockout or modification of human hepatoma cell line IGF-IR gene on cellular level is realized successfully, hepatoma carcinoma cell IGF-IR gene transcriptional level is reduced obviously, hepatoma carcinoma cell proliferation, invasion, and migration capacities are reduced, a novel method is provided for treatment of hepatic carcinoma, and clinical application prospect is promising.

The invention discloses coated type nanoscale zero-valent iron as well as a preparation method and an application thereof. The coated type nanoscale zero-valent iron comprises nanoscale zero-valent iron and sodium alginate, wherein the nanoscale zero-valent iron is coated with sodium alginate, and a mass ratio of sodium alginate to the nanoscale zero-valent iron is (0.1-0.4):1. The preparation method of the coated type nanoscale zero-valent iron comprises the step that the nanoscale zero-valent iron and a sodium alginate solution are mixed and subjected to ultrasonic treatment, and the coated type nanoscale zero-valent iron is obtained. The coated type nanoscale zero-valent iron can be used for treating sediment with heavy metal pollution, is simple and convenient to operate, good in stabilizing effect, good in mobility, low in repair cost, clean and pollution-free, has no toxic and harmful effects on the environment and has broad application prospect in the technical field of treatment of heavy metal pollution of the sediment.

The invention provides a curing-stabilization chemical for restoring arsenic-polluted soil. The curing-stabilization chemical comprises, by weight percentage, 10-15% of an oxidizing agent, 20-40% of a curing agent, 22-45% of a stabilizing agent, 10-35% of additives and 3-8% of a synergist. By means of the curing-stabilization chemical, the existing form of arsenic is changed, the arsenic is fixed into the soil through the oxidoreduction effect, the precipitation effect and the adsorption effect, and chemical properties of the arsenic are stabilized; mobility and bio-availability of the arsenic are lowered; and through five types of synergistic interaction effects, the restoration effect is good. The arsenic-polluted soil is treated and restored with the chemical; the total arsenic concentration of arsenic slag sample leachate is 294 mg/L, and the pH value is 7.5; after treatment, the total arsenic concentration of the leachate is 2.3 mg/L, the pH value is 5.0, and the arsenic fixing rate is as high as 99.86%; and meanwhile, the restoration standard, namely 2.5 mg/L, specified in 'Standard for Pollution Control on the Security Landfill Site for Hazardous Wastes' is reached.

The invention relates to the field of soil heavy metal pollution remediation and specifically discloses an APP combined remediation method for cultivated land heavy metal pollution remediation. According to the APP combined remediation method, the chemical technology and the engineering technology are combined, and the operable and propagable APP combined remediation method comprising a soil conditioner (Amendment), power of hydrogen adjustment (pH) and deep ploughing (Plough) is formed. Through the combined remediation method, on the premise that the soil conditioner product which uses natural mineral raw materials and is efficient in remediation, low in cost and free of secondary pollution to the environment is provided, the nature of crops planted in cultivated land is not changed, heavy metals in the soil are passivated and stabilized, absorption of the crops to the heavy metals is suppressed and hindered, enrichment of the heavy metals in the crops is reduced, meanwhile, medium elements such as calcium and silicon are provided for growth of the crops, and safety production of agricultural products is achieved.

The invention relates to an F<->, Zn<2+>, and B<3+> co-doped NASICON type solid lithium-ion electrolyte, which is characterized by having a stoichiometric equation of Li1+2x+2z-yZnxBzM2-xP3-zO12-yFy, wherein, x=0.1-0.5; y=0.1-0.2; z=0.1-0.3; M is one of Ti, Ge and Zr. According to the invention, ZnO, LiF, B2O3, MO2 (M=Ti, Ge, Zr), NH4H2PO4, and Li2CO3 are mixed uniformly in a mole ratio of 0.1-0.5:0.1-0.2:0.05-0.15:1.5-1.9:2.7-2.9:0.5-1.2, then the obtained mixture is subjected to ball milling, pressing and sintering, thus obtaining a solid lithium-ion electrolyte with room temperature lithium ion conductivity higher than 9.10<-4>S/cm.

The invention provides a soil cadmium passivator. The soil cadmium passivator is prepared from biological carbon, quick lime and sodium alginate; by means of the quick lime in the soil cadmium passivator, a pH value of soil can be improved, available cadmium in an ionic state is converted into a cadmium sediment in an invalid state such as cadmium hydroxide; the sodium alginate has a good chelating effect on heavy metal cadmium; the biological carbon has certain adsorbing effects on the available cadmium in the ionic state, the cadmium hydroxide and invalid cadmium such as in cadmium a complexing state; the biological carbon, the quick lime and the sodium alginate in the soil cadmium passivator can be mutually matched and cooperated at a specific mixture ratio, so that the effects of reducing the content of the cadmium in an available state in soil and the content of the cadmium in a living body grown in the soil are realized; the soil cadmium passivator also has the advantages that a PH value in the soil is improved, the functions of the soil are improved, and secondary pollution is avoided.

The invention relates to a stabilizer for heavy metal compound contaminated soil and a preparation method of the stabilizer for heavy metal compound contaminated soil. The stabilizer comprises sulfide, shell powder and red mud, wherein the molar ratio of the sulfide to effective-state gross of heavy metals lead, zinc and cadmium is 1.5 to 6; the molar ratio of the shell powder to the effective-state gross of heavy metals lead, zinc and cadmium is 1-4; and the red mud is added in a molar ratio of the content of iron in the red mud to the effective-state content of arsenic in soil of 2-8. The preparation method of the stabilizer comprises the following steps: drying the red mud by airing, grinding and sieving the dried red mud; then, cleaning the shell powder, grinding and sieving after drying by air; and preparing the sulfide into an aqueous solution, thereby obtaining the stabilizer. Based on the effective-state content of the heavy metals in the soil, the content of each component is reasonably prepared, so that the stabilizer can be applicable to compound contaminated soil with high leaching concentration, can be used for effectively reducing the leaching concentration of heavy metals in the soil and overcoming the defect of poor stabilization effects of the existing medicament, and has a certain application value.

The invention discloses a cadmium-arsenic composite polluted soil remediation agent, which comprises the following raw materials in parts by weight: 0-10 parts of hematite; 20-30 parts of ferrous sulfate; 10 parts of manganese dioxide; 15-20 parts of fly ash; 10-15 parts of quicklime; 10-15 parts of sepiolite; 10-15 parts of calcium dihydrogen phosphate; and 0-10 parts of wollastonite. The remediation agent enables cadmium and arsenic heavy metals to form stable chemical forms through oxidation reduction, adsorption, ion exchange, precipitation or coprecipitation and the like to reduce the migration and diffusion capability of the cadmium and arsenic heavy metals in the environment, so that the ecological environment risk of the cadmium and arsenic heavy metals is reduced; and the remediation agent has the advantages of high remediation efficiency for cadmium-arsenic composite polluted soil, short remediation period, low remediation cost, easily available raw material components and simple application method, so that the agent is one of the few remediation agents in the industry that simultaneously act on yin and yang heavy metal ions.

The invention discloses application of a reagent for inhibiting circ_CLASP2 (Cytoplasmic Linker Associated Protein 2) in preparation of a preparation for treating nasopharyngeal darcinoma and the preparation. A research proves that the circ_CLASP2 is inhibited in nasopharyngeal darcinoma cells, and the proliferation, invasion and metastasis of the nasopharyngeal darcinoma cells can be inhibited. Therefore, a circ_CLASP2 inhibitor is used for treating the nasopharyngeal darcinoma and has profound clinical significance and an important popularization and application prospect.

The invention provides a soil conditioner and an application method thereof. The soil conditioner is prepared from the following raw materials in parts by weight: 0 to 50 parts of calcium fertilizer,0 to 30 parts of magnesium fertilizer, 0 to 40 parts of potassium fertilizer, 5 to 50 parts of silicon fertilizer, 5 to 10 parts of humic acid and 5 to 10 parts of attapulgite. Compared with the priorart, production raw materials of the soil conditioner disclosed by the invention are prepared from natural minerals, have lost cost and are convenient to take. The produced soil conditioner are prepared from the calcium fertilizer, the magnesium fertilizer, the potassium fertilizer, the silicon fertilizer and the like and can effectively activate and supplement nutrients of calcium, phosphorus, potassium and the like which are fixed by acidification for soil; in use, the produced soil conditioner can achieve a balanced fertilizing effect. The soil conditioner has the advantages of simple production mode, easiness, practicability and wide popularization prospect.

The invention provides a soil conditioner and a preparation method thereof. The soil conditioner comprises, by weight, 12 to 20 parts of plant ash, 5 to 10 parts of phosphorous slag, 5 to 9 parts of quicklime, 2 to 5 parts of zeolite, 2 to 5 parts of attapulgite, 1 to 3 parts of Yilong seaweed ooze, 2 to 5 parts of Chinese magnoliavine residues, 2 to 5 parts of large-flowered skullcap root residues, 20 to 30 parts of straw, 8 to 13 parts of hickory nut shells, 0.02 to 0.04 part of rotenone, 10 to 15 parts of mangosteen shells, 3 to 5 parts of an EM microbial agent, 7 to 20 parts of urea phosphate, 8 to 14 parts of urea, 10 to 15 parts of biochemical potassium fulvate and 9 to 12 parts of gamma-polyglutamic acid. The invention has the beneficial effects that the soil conditioner has high porosity and has specific adsorption effect on heavy metal ions; the soil conditioner can adjust the pH value of soil, is rich in trace elements and can elemental N, elemental P and elemental K at the same time; and the soil conditioner allows soil to maintain high porosity and to be long in fertility maintenance time and good in water retention performance.

The invention relates to an acid soil regulator with a plant nutrient function and a preparation method thereof. The acid soil regulator is prepared by uniformly mixing basic materials and auxiliary materials, wherein the basic materials are prepared by mixing and fermenting brewer's grains, peat soil, traditional Chinese medicine dregs, soybean meal powder, straw powder, edible fungi dregs, pineapple paste, apple paste, silkworm excrement, proteolytic enzyme, nitrogen-immobilizing bacteria, rhizobium, potassium-decomposing bacteria, cellulose decomposing bacteria, antibiotic producing bacteria and water; the auxiliary materials are prepared by uniformly mixing earthworm manure, charcoal residues, urea, plant ash, lime powder, urea iron complex, and chelated zinc. The preparation method comprises the following steps of A, preparation of the basic materials; B, preparation of the auxiliary materials; C, preparation of the soil regulator. The acid soil regulator has the advantages that multiple rich and absorbable nutrients are provided for the plant growth, the problem of soil caking is effectively solved, the air ventilating property of the soil is improved, the acidity of the soilis reduced, and the fertilizer-maintaining and water-maintaining ability of the soil is enhanced. The preparation method has the advantages that the preparation method is simple, the implementing iseasy, the investment cost is low, and the preparation method is suitable for large-area popularizing.

The invention belongs to the technical field relevant to soil remediation and particularly relates to a microwave catalytic remediation method for organic matter-heavy metal compound contaminated soil. The microwave catalytic remediation method for the organic matter-heavy metal compound contaminated soil comprises the steps that magnesium salt, ferric salt and aqueous alkali are added into deionized water and then mixed and stirred; turbid liquid is irradiated in a microwave oven; suction filtration is conducted on the turbid liquid after a reaction so that sediment can be separated out, and the sediment is washed and dried; a microwave catalyst is obtained through cooling and grinding after calcination; the microwave catalyst and the contaminated soil are mixed; the mixture is placed in a microwave reactor, and oxygen or air is led into the microwave reactor for a microwave reaction; and gas generated during the reaction is collected at the same time, and thus required remediation of the contaminated soil is completed. By the adoption of the microwave catalytic remediation method for the organic matter-heavy metal compound contaminated soil, the contaminated soil can be remediated in the way that environment protection is facilitated, quality control is convenient and the efficiency is high; and the microwave catalyst is used for adsorbing heavy metal ions in the contaminated soil and degrading organic matter in the contaminated soil, and meanwhile secondary pollution is avoided by collecting waste gas.

The invention relates to a compound repair agent for heavy metal polluted soil which is characterized by being prepared from the following raw materials in percent by weight: 40-55% of charcoal, 15-35% of bone powder and 15-35% of coal ash. By taking agricultural or industrial solid wastes which are wide in source and easy to obtain as a raw material, the compound repair agent is simple in process, sufficient in raw material, low in cost and obvious in application effect, reduces the heavy metal mobility and bioavailability of soil, and can solve the problem of sustainable utilization of heavymetal pollution in farmlands and fields of industrial and mineral industry. The application method is simple, the using process is labor-saving, the physical and chemical properties of soil can be also improved effectively, the fertilizer retaining and water retaining ability of soil is improved, and the compound repair agent is easy to popularize and apply.

The invention provides a stable solidification method of arsenic-containing waste residues. According to the method provided by the invention, the arsenic-containing waste residues and a soil solidifying agent are combined, so that the arsenic-containing waste residues are solidified in a high-strength solidified body, and the leaching toxicity of the arsenic-containing waste residues is reduced;the leaching rate of solidified arsenic can reach related requirements of Identification Standards for Hazardous Wastes-Identification for Extraction Toxicity (GB5085.3-2007), so that the arsenic-containing waste residues are changed into common solid wastes, stable solidification treatment of the arsenic-containing waste residues is realized, and furthermore, the aim of safety stacking is realized; the method is simple to operate, economical and environmentally friendly; the environment pollution problem caused by the arsenic-containing waste residues in the prior art is solved.

The invention discloses a soil remediation adsorption net and a remediation method thereof. The soil remediation adsorption net comprises a net plate. The net plate is formed by pressing an adsorption material and is in a wave shape. The thickness of the net plate is 2-5 mm. Strip-shaped filter holes are evenly formed and distributed in the surface of the net plate. An inclination angle of 10-15 degrees is formed between each strip-shaped filter hole and a corresponding wavy folded edge of the net plate. The adsorption material is prepared from ion exchange fibers and attapulgite powder, wherein the weight ratio of the ion exchange fibers to the attapulgite powder is 2-4:1-1.5. The remediation method comprises the step that the adsorption net is embedded into the soil with the depth being 15-25 cm for 15-30 days. The adsorption net is prepared from the attapulgite powder and the exchange fibers and applied to government and remediation of soil pollution. The remediation method is simple and fast and suitable for industrial production. Harmful substances in the soil can be removed permanently through adsorption and exchange, the purpose of remedying soil pollution is achieved, the leaching concentration of the harmful substances in the soil is lowered, and the mobility and poisoning degree of heavy metal in the soil are reduced.

The invention discloses a method for stabilizing phosphorus and heavy metal in sludge of a sewage treatment factory, and relates to the preparation of an activated red mud stabilizer and the method for stabilizing the phosphorus and the heavy metal in the sludge of the sewage treatment factory. The method has the main technical characteristics that: the activated red mud stabilizer is prepared by roasting cheap bauxite residue red mud serving as a raw material at a certain temperature, and is stirred and mixed uniformly with the sludge of the sewage treatment factory, and the phosphorus and the heavy metal in the excess sludge can be effectively stabilized after the mixture is cultured for a certain period of time. The product performance is that: the specific surface area of the prepared red mud stabilizer is 16.48 to 25.78m<2>/g, and the efficiency of stabilizing the phosphorus and the heavy metal is over 70 percent. The method is simple and practicable, high in treatment efficiency and low in treatment cost.

The invention provides a composite stabilizer for contaminated soil. The composite stabilizer comprises the following components in parts by weight: 20 to 30 parts of iron powder, 2 to 7 parts of aluminum powder, 45 to 55 parts of calcium oxide and 15 to 25 parts of sepiolite. The technical scheme has the effects that the composite stabilizer consists of the iron powder, the aluminum powder, the calcium oxide and the sepiolite, and the iron powder and the aluminum powder can be used for reducing arsenic and cadmium in an environment and reducing toxicity, and can also form a complex and a compound with the arsenic and the cadmium to reduce the migration capability; the sepiolite has a large specific surface area, can be used for adsorbing a large number of substances, is an excellent loading carrier, and has a catalytic effect; the calcium oxide can be used for regulating a reaction condition to be alkaline, and heavy metals can be precipitated more easily under the alkaline condition; a unique proportion is adopted, so that optimal stabilization effects of the composite stabilizer on the arsenic and the cadmium in the contaminated soil can be achieved under the condition of lowest cost. The invention also provides a treatment method for the contaminated soil. The method comprises simple steps, and is high in practicability, and parameters are easy to control.