Efficient oil-producing filamentous fungus mucor circinelloides Q-531 and application thereof

A technology of filamentous fungus and Mucor crimperides, applied in the field of agricultural and forestry waste reuse, can solve the problem of low cellulase activity, achieve the effects of increasing oil production, facilitating industrialization, and reducing production costs

Active Publication Date: 2017-05-10
NANJING FORESTRY UNIV
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Problems solved by technology

A. tubingensis TSIP9 directly biotransforms PPF and EFB into oil, and the oil yields are 31.1mg / gds and 37.5mg / gds, respectively. However, the cellulase activity of this strain is very low, less than 1.3U / gds
Two researchers, Cheirsilp and Kitcha, continued to study the oil production of A. tubingensis TSIP9 solid-state fermentation. Using the mixture of EFB and PK, the oil production reached 39.5mg / gds, but the cellulase activity was still very low, only 2.35U / gds

Method used

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  • Efficient oil-producing filamentous fungus mucor circinelloides Q-531 and application thereof
  • Efficient oil-producing filamentous fungus mucor circinelloides Q-531 and application thereof
  • Efficient oil-producing filamentous fungus mucor circinelloides Q-531 and application thereof

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Embodiment 1

[0023] The configuration of the solid-state fermentation medium: spread out the mulberry branches and place them in an open space, air-dry them for 5 days to remove their moisture, then cut them into 2-3cm strips with scissors, pulverize them with an ultrafine pulverizer, and pass through a 40-mesh sieve to obtain The powder is placed in an air-blast drying oven at 80°C, dried for 24 hours, and then stored in a desiccator for use; weigh 30g of mulberry branch powder into a 250mL Erlenmeyer flask, and add 90mL of MS solution to keep the humidity of the medium At 70-80%. The configuration of the MS solution is (NH 4 ) 2 SO 4 1.7g, KH 2 PO 4 2.0g, MgSO 4 ·7H 2 O 0.5g, CaCl 2 2H2O 0.2g, FeSO 4 ·7H 2 O 0.01g, ZnSO 4 7H2O 0.01g, MnSO 4 4H 2 O 0.001g, CuSO 4 ·5H 2 O 0.0005g and 0.1% Tween-80 (w / v), add 1000mL distilled water, pH6.0.

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Abstract

The invention discloses an efficient oil-producing filamentous fungus mucor circinelloides Q-531 and an application thereof. The efficient oil-producing filamentous fungus is classified and named as mucor circinelloides Q-531 and is preserved in China Center for Type Culture Collection; the preservation number is CCTCC M2016364; the preservation date is on July 4th, 2016; the preservation address is at Wuhan University of China. According to the invention, a solid state fermentation process is adopted for directly converting mulberry branches into microbial oil; the highest oil yield can reach up to 42.43+ / -4.01mg / gds; the highest cellulase enzyme activity can reach up to 1.39+ / -0.09 FPU / gds. Besides, the highest oil content in fungus cell is 28.8+ / -2.85%. According to the invention, the mucor circinelloides research is utilized to produce oil by fermenting forestry and agricultural residues (mulberry branches) under solid state, so that the waste resources is reasonably utilized, the oil yield is increased, the production cost is reduced and the industrialization is benefited.

Description

technical field [0001] The invention relates to the technical field of reuse of agricultural and forestry waste, in particular to a high-efficiency oil-producing filamentous fungus Mucor circinosa Q-531 and its application. Background technique [0002] With the development of human society and economy, energy crisis and environmental protection have become two major global issues at present. Facing the rapid consumption of fossil fuels, the non-renewable resources on the earth are becoming less and less, and more and more countries are urgently seeking new and renewable energy sources to meet their own needs. As the most abundant resource on earth, lignocellulosic biomass is considered to be a promising alternative raw material precisely because of its abundant, cheap and renewable characteristics. According to data, the annual plant growth in the world is as high as 1.6×10 11 tons dry weight. As a part of lignocellulosic biomass, agricultural and forestry wastes, such a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12P7/64C12R1/785
CPCC12P7/6463C12P7/6472C12N1/145C12R2001/785
Inventor 乔维川陶骏奇李梦婷谢震宇夏昊葛秀秀柏涵刘海
Owner NANJING FORESTRY UNIV
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