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Mucor circinelloides CAULIU-FUNGUS-1 and application thereof

A technology of Mucor circinigii, cgmccno.21479, which is applied in the field of composition, bacterial agent, water-phase product of hydrothermal conversion, and Mucor circinifolia, which can solve the problems of restricting the green development of hydrothermal conversion technology

Active Publication Date: 2021-09-03
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current treatment of the water phase has become an important obstacle to the green development of hydrothermal conversion technology

Method used

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  • Mucor circinelloides CAULIU-FUNGUS-1 and application thereof
  • Mucor circinelloides CAULIU-FUNGUS-1 and application thereof
  • Mucor circinelloides CAULIU-FUNGUS-1 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0081] Example 1 Isolation, purification and preservation of Mucor circinosa CAULIU-FUNGUS-1

[0082] 1. Preparation of culture medium:

[0083] PDA medium: Wash and peel the potatoes first, then weigh 200g of potatoes and cut them into small pieces, boil them in water, and filter them with gauze. Heat, add 15-20g of agar according to the actual experiment needs, continue to heat and mix well, after the agar is dissolved, add 20g of glucose, stir evenly, after cooling slightly, make up water to 1000ml, and sterilize at 115°C for 20 minutes.

[0084] PDB medium: Wash and peel the potatoes first, then weigh 200g of potatoes and cut them into small pieces, add water to boil, filter with gauze, add 20g of glucose, stir evenly, after cooling down slightly, add water to 1000ml, and sterilize at 115°C 20 minutes.

[0085] 2. Screening, isolation, purification and preservation of strains

[0086] Sample Collection

[0087] Mucor volvulus CAULIU-FUNGUS-1 was obtained by screening, ...

Embodiment 2

[0093] Example 2 Degradation of microalgae by using Mucor circinatus CAULIU-FUNGUS-1 for hydrothermal transformation of water phase

[0094] Taking the water phase product of microalgae hydrothermal conversion as the processing object, the property parameters of the water phase product of microalgae hydrothermal conversion are as follows: pH8.8, chemical oxygen demand (COD) is 101.1g / L, total nitrogen (TN) content is 22.3 mg / L.

[0095] The method for degrading microalgae hydrothermal conversion water phase by using Mucor circinatus CAULIU-FUNGUS-1 comprises the following steps:

[0096] (1) Inoculate Mucor circiniferatum into PDA medium, culture at 28°C for 48 hours, and prepare fungus cake with a 6mm hole punch.

[0097] (2) Inoculate 5 fungus cakes from step (1) into 50 ml of PDB medium containing 20% ​​hydrothermal conversion water phase product, and shake culture at 28°C.

[0098] (3) On the 0th day, the 5th day, and the 10th day, respectively, take 2ml of the culture a...

Embodiment 3

[0111] Example 3 Degradation of straw by using Mucor circinosa CAULIU-FUNGUS-1 for hydrothermal conversion of water phase

[0112] Taking the water phase product of straw hydrothermal conversion as the processing object, the property parameters of the water phase product of straw hydrothermal conversion are as follows: pH3.8, chemical oxygen demand (COD) is 81.1g / L.

[0113] The method for degrading straw by using Mucor circinatus CAULIU-FUNGUS-1 for hydrothermal transformation of water phase comprises the following steps:

[0114] (1) Inoculate Mucor circiniferatum into PDA medium, culture at 28°C for 48 hours, and prepare fungus cake with a 6mm hole punch.

[0115] (2) Inoculate 5 fungus cakes from step (1) into 50 ml of PDB medium containing 20% ​​hydrothermal conversion water phase product, and shake culture at 28°C.

[0116] (3) On the 0th day, the 5th day, and the 10th day, respectively, take 2ml of the culture and centrifuge at 12000rpm for 10min, measure the COD of th...

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Abstract

The invention provides Mucor circinelloides which is preserved in China General Microbiological Culture Collection Center on April 9, 2021, with the preservation number of CGMCC No. 21479 and the classification name of Mucor circinelloides, wherein the preservation address is China General Microbiological Culture Collection Center of Institute of Microbiology, Chinese Academy of Sciences, 3#, No. 1 Yard, West Beichen Road, Chaoyang District, Beijing. The Mucor circinelloides can grow in a hydrothermal conversion aqueous phase product under proper conditions, and can convert multiple substances in hydrothermal conversion and reduce the toxicity of the hydrothermal conversion aqueous phase product.

Description

technical field [0001] The present invention relates to the field of biology, specifically, the present invention relates to Mucor circinosa CAULIU-FUNGUS-1 and its application, and more specifically, the present invention relates to Mucor circinosa, a composition, a fungal agent, and a hydrothermal conversion water phase product Methods. Background technique [0002] The use of hydrothermal conversion technology can convert low-energy-density biomass resources such as crop straw, kitchen waste, municipal sludge, and livestock and poultry manure into high-energy-density bio-crude oil and biochar. The hydrothermal conversion products include gas phase, oil phase, water phase and solid phase. After hydrothermal conversion, the elements in the biomass raw material will be redistributed in different products, and part of the carbon and nitrogen in the raw material will be transferred to the water phase product. Lead to higher COD (chemical oxygen demand) content in the aqueous ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C02F3/34C12R1/785C02F101/10C02F101/34C02F101/38
CPCC12N1/14C02F3/34C02F2101/345C02F2101/34C02F2101/38C02F2101/105
Inventor 刘志丹徐永洞王月瑶陈春琳袁昌斌
Owner CHINA AGRI UNIV
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