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Mucor circinelloides, method for preparing viscosity-reduction enzyme and application of viscosity-reduction enzyme

A technology of Mucor circiniferi and viscosity-reducing enzyme, which is applied in Mucor circinia to prepare viscosity-reducing enzyme and the field of common Fusarium, which can solve the problems that are not conducive to the large-scale production of fuel ethanol, reduce the production of alcohol, Increase the amount of fermentation water, etc., to achieve the effects of short action time, good viscosity reduction effect, and high activity

Active Publication Date: 2014-03-05
CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These two methods all have their own technical defects: the former not only increases the water consumption of fermentation, but also reduces the production of alcohol, which cannot solve practical problems; the latter uses commercial enzymes as a fast pretreatment method, which increases the production cost, Not conducive to large-scale production of fuel ethanol

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] This example describes the screening method of Mucor circiniferus CBS131818.

[0034] Dry the rice straw at 60°C to constant weight, crush it, and pass through a 35-mesh sieve; take 10 g of the screened powder and put it in a 250 ml Erlenmeyer flask, add 100 ml of dilute sulfuric acid with a concentration of 0.5% (v / v), and heat up to 121 Keep at ℃ for 60 minutes to get the treatment solution; if the treatment solution is left at room temperature for 1 to 2 months, mold will grow on the liquid surface.

[0035] The screening process is as follows: according to the classic coating and dilution plate method, the above treatment solution is passed through 10 -1 、10 -2 、10 -3 、10 -4 、10 -5 、10 -6 、10 -7 After diluting the concentration, take 100μL10 -5 , 10 -6 , 10 -7 Spread the bacterial suspension at a certain concentration on the screening medium, culture at 30°C for 3 days, pick a single colony that grows larger on the plate and put it on the PDA slant medium, ...

Embodiment 2

[0040] This example describes the screening method of Mucor circiniferus CBS131818, which is the same as Example 1 and will not be repeated, except that the concentration of dilute sulfuric acid is 4% (v / v).

Embodiment 3

[0042] This example describes the screening method of Mucor circiniferus CBS131818, which is the same as Example 1 and will not be repeated, except that the concentration of dilute sulfuric acid is 2% (v / v).

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PUM

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Abstract

The invention discloses mucor circinelloides, a method for preparing viscosity-reduction enzyme and application of viscosity-reduction enzyme, and belongs to the field of microbe and microbial fermentation. Aiming at the disadvantages of reducing sweet potato fermentation liquor viscosity in the prior art, the invention provides a strain capable of producing viscosity-reduction enzyme. Mucor circinelloides CBS131818 is preserved in Royal Netherlands Academy of Arts and Sciences Centraalbureauvoor Schimmelcultures, and the preservation date is Jan. 27, 2012. Mucor circinelloides CBS131818 is acquired from acid-processed paddy rice straw treating fluid and is applicable to preparation of viscosity-reduction enzyme. The produced viscosity-reduction enzyme is high in activity, is further applicable to viscosity reducing technology of sweet potato fuel ethanol fermentation liquors, is capable of effectively reducing the viscosity of the sweet potato stem-tuber fermentation liquor with small use amount and short action time, and helps to simplify the sweet potato fuel ethanol fermentation technology and reduce production cost.

Description

technical field [0001] The present invention relates to a kind of Mucor volvulus, a method for preparing a viscosity-reducing enzyme by using the strain and its application, in particular to a common Fusarium which can be applied to the fermentation of sweet potato tubers for reducing viscosity and its application, belonging to microorganisms and microbial fermentation field. Background technique [0002] Biomass fuel ethanol refers to the production of absolute ethanol by fermenting biomass materials such as plants, and then adding a denaturant (generally unleaded gasoline or unleaded hydrocarbons) with a volume ratio of about 5% to make it a water content of less than 0.8%. Edible denatured absolute ethanol. Fuel ethanol is a good gasoline oxygenator and octane number blending component, which can effectively reduce the carbon monoxide content in automobile exhaust, so as to truly realize energy saving and emission reduction. Biomass fuel ethanol, as a renewable energy s...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12N9/42C12N9/24C12P7/06C12R1/785
CPCY02E50/17Y02E50/10
Inventor 赵海靳艳玲方扬黄玉红
Owner CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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