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Mucor circinelloides DK1 strain and application thereof

A technology of Mucor cuspidatum and strain, applied in the field of Mucor cuspidatum DK1 strain, can solve the problems of high energy consumption of chemical degumming process, unrecyclable waste water, large equipment loss, etc., and achieves high degumming efficiency, short degumming cycle, good quality effect

Inactive Publication Date: 2015-06-24
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the scouring methods commonly used in the hemp industry are mainly chemical methods and enzymatic degumming methods. The chemical degumming process consumes a lot of energy and equipment loss, and the discharged wastewater cannot be recycled, causing serious pollution problems.

Method used

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  • Mucor circinelloides DK1 strain and application thereof
  • Mucor circinelloides DK1 strain and application thereof
  • Mucor circinelloides DK1 strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Screening method for retting or scouring strains of hemp fiber and bamboo fiber:

[0040] (1) Decomposed hemp and its soil were obtained from the retting base, and the decomposed hemp and its soil and the PDA medium were placed in the PDA medium at a ratio of 1 g: 20 ml, and enriched for 10 days. Then take 0.1ml of the PDA culture and apply it to the separation medium, and culture it statically at 30°C for 1-4 days to obtain a wild-type biologically treated high-quality strain.

[0041] (2) Inoculate the bacterial strain obtained in step (1) into PDA medium, and culture at 30° C. for 72 hours. Add freezing buffer and store at -80°C.

[0042] Described step (1) PDA medium formula is: potato 200g, glucose 20g, agar 15g, distilled water 1000mL, pH is natural.

[0043] The formula of step (2) per 100ml of freezing buffer: potassium dihydrogen phosphate 0.1g, dipotassium hydrogen phosphate 0.02g, sodium citrate 0.06g, magnesium sulfate heptahydrate 0.03g, glycerol 12ml, ad...

Embodiment 2

[0046] Utilize the method for preparing laccase by Mucor circinarius DK1 bacterial strain:

[0047](1) The Mucor circinatus DK1 strain is stored in PDA medium, and a freezing buffer is added;

[0048] (2) Into the sterilized and cooled 50ml PDA medium, insert the bacterium ring obtained in step (1), shake the flask at a rotating speed of 200rpm at 30°C for 24 hours; after cultivating the 50ml shake flask, inoculate the culture medium Add 5L of flax, jute or kenaf fermentation medium, and shake the flask for 24 hours under the conditions of a rotating speed of 200r / min and a temperature of 30°C to obtain a fermentation broth;

[0049] (3) Centrifuge the fermentation broth obtained in step (3) at 4° C. at 8,000 r / min, and take the supernatant, which is laccase. Then determined by spectrophotometry, the laccase activity is 10-30IU / ml, and the enzyme activity is relatively stable at pH 5.0-6.0 and 20-60°C.

[0050] Enzyme activity assay method, using guaiacol as laccase substrat...

Embodiment 3

[0052] The retting process of flax, jute or kenaf is as follows:

[0053] (1) Preserve the Mucor circinatus DK1 strain in PDA medium (per 1000ml of medium includes 200g of potato, 20g of glucose, 15g of agar, and 1000mL of distilled water), and add freezing buffer. The formula of every 100ml of freezing buffer of described step: potassium dihydrogen phosphate 0.1g, dipotassium phosphate 0.02g, sodium citrate 0.06g, magnesium sulfate heptahydrate 0.03g, glycerin 12ml, add water and be settled to 100ml, prepare have to.

[0054] (2) Insert a ring of bacteria into 1000ml of flax, jute or kenaf bran medium (per 1000ml medium includes 200g of flax, jute or kenaf bran, 50g of bran, 50ml of wort and 950ml of water), shake the flask Culture, the culture condition is 30°C, the pH is natural, and the culture time is 24 hours;

[0055] (3) Inoculate the culture medium into flax, jute or kenaf bran fermentation medium (every 1000ml culture medium includes flax, jute or kenaf bran 100g, ...

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Abstract

The invention relates to a mucor circinelloides DK1 strain and an application thereof. An ITS (internal transcribed spacer) sequence of the strain is shown by SEQ ID NO:1. The strain is applied to preparation of flax, jute, kenaf or bamboo fiber by retting, boiling-off of flax, jute, kenaf or bamboo yarns, and degumming of flax, jute, kenaf original hemp or bamboo fabrics. The strain provided by the invention is short in growth cycle, cannot be polluted easily, is low in treatment cost, good in fiber quality after treatment, mild in treatment environment, good in heat resistance and free from environmental pollution; and the strain can be directly applied to degumming of the flax, jute, kenaf and bamboo fibers, and is short in degumming cycle, high in fiber dispersion rate and high in degumming efficiency.

Description

technical field [0001] The invention belongs to the technical field of bio-textiles, and in particular relates to a strain of Mucor circiniferus DK1 and an application thereof. Background technique [0002] Mucor volvulus exists in the environment such as soil, manure, grass and air. It grows well in conditions of high temperature, high humidity and poor ventilation. Mucor volvulus has a wide range of uses and can produce pectinase, xylanase, laccase, rennet, etc. Commonly used Mucor are mainly Mucor ruckeri and Mucor racemosa. Saprophytic, widely distributed in plant residues, decaying organic matter, animal manure and soil. [0003] In the primary processing of hemp, it is necessary to pre-treat part of the gum (mainly pectin and hemicellulose) through a retting process. The retting process actually uses traditional microbial technology, which is a process of slowly removing non-cellulose gum attached to the fiber surface through the synergistic action of microorganism...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14D01C1/04D01C1/00C12R1/785
CPCC12N1/145C12R2001/785D01C1/00D01C1/04
Inventor 丁若垚郁崇文张兴群原月梦孔德枝常慧
Owner DONGHUA UNIV
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