Microbial agent for treating heavy metal polluted soil, preparation method and applications thereof

A microbial agent and heavy metal technology, applied in the field of soil remediation, can solve the problems of lack of soil remediation technology, limited application range, difficult growth of animals and plants, etc., and achieve the effect of controlling heavy metal pollution, diverse remediation mechanisms, and increasing the scope of application

Active Publication Date: 2017-02-22
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The dumps, tailings ponds and surrounding polluted sites formed by mining activities often have complex pollutant components, containing heavy metals and various organic pollutants. These heavy metals have high pollution concentrations and serious pollution levels, making it difficult for animals and plants to grow in them , seriously affecting the ecological environment
Heavy metals in the soil can pollute the surrounding land, waters and atmosphere through surface water runoff and atmospheric dust, and its impact will far exceed the area and space of the waste dump. The impact of pollution will cost a lot of manpower, material resources and financial resources It takes a long time to recover, and it is difficult to return to the original level
[0003] Modern technology to deal with heavy metal pollution soil remediation technology is relatively scarce, mainly focusing on the enrichment of specific heavy metals by hyperaccumulative plants. This method can only deal with relatively single heavy metal pollution, and plants have high requirements on the soil environment, which limits its application. scope

Method used

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  • Microbial agent for treating heavy metal polluted soil, preparation method and applications thereof
  • Microbial agent for treating heavy metal polluted soil, preparation method and applications thereof
  • Microbial agent for treating heavy metal polluted soil, preparation method and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] A microbial agent for treating soil pollution by heavy metals, consisting of the following raw materials by weight:

[0045] 80 parts of rice husk, 20 parts of pig manure, 50 parts of chicken manure, 30 parts of soybean meal, 6 parts of single cell protein, 2.5 parts of Mortierella alpina fermentation broth, 2.5 parts of Trichoderma asperellum fermentation broth Parts, 0.5 parts of Mucor circinelloides fermentation broth;

[0046] The total content of bacteria in the microbial agent is 5.3×10 8 cfu / g; the ratio of the number of viable cells of Mortierella alpina (Mortierella alpina), Trichoderma asperellum (Trichoderma asperellum) and Mucor circinelloides (Mucor circinelloides) is 1:1.8:2.5.

[0047] The method for preparing the above-mentioned microbial inoculum for treating heavy metal pollution of soil has the following steps:

[0048] (1) Mortierella alpina was cultivated by primary seed culture, secondary seed culture and expanded culture to obtain 0.4×10 8 cfu / ml Mortiere...

Embodiment 2

[0060] A microbial agent for treating soil pollution by heavy metals, consisting of the following raw materials by weight:

[0061] 50 parts of rice husk, 50 parts of pig manure, 20 parts of chicken manure, 60 parts of soybean meal, 4 parts of single cell protein, 3.5 parts of Mortierella alpina fermentation broth, and 1.5 parts of Trichoderma asperellum fermentation broth 1 part, 1.5 parts of Mucor circinelloides fermentation broth;

[0062] The total content of bacteria in the microbial agent is 8.3×10 8 cfu / g; the ratio of the number of viable cells of Mortierella alpina (Mortierella alpina), Trichoderma asperellum (Trichoderma asperellum) and Mucor circinelloides (Mucor circinelloides) is 1:1:3.6.

[0063] The preparation method is the same as in Example 1.

Embodiment 3

[0065] A microbial agent for treating soil pollution by heavy metals, consisting of the following raw materials by weight:

[0066] 65 parts of rice husk, 35 parts of pig manure, 35 parts of chicken manure, 45 parts of soybean meal, 5 parts of single-cell protein, 3 parts of Mortierella alpina fermentation broth, and Trichoderma asperellum fermentation broth 2 1 part, 1 part of Mucor circinelloides fermentation broth;

[0067] The total content of bacteria in the microbial agent is 6.8×10 8 cfu / g; the ratio of the number of viable cells of Mortierella alpina (Mortierella alpina), Trichoderma asperellum (Trichoderma asperellum) and Mucor circinelloides (Mucor circinelloides) is 1:1.5:3.

[0068] The preparation method is the same as in Example 1.

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Abstract

The present invention relates to a microbial agent for treating heavy metal polluted soil, a preparation method and applications thereof. The microbial agent comprises the following raw materials: rice husk, pig manure, chicken manure, soybean powder, single cell protein, a Mortierella alpina fermentation broth, a Trichoderma asperellum fermentation broth, and a Mucor circinelloides fermentation broth. According to the present invention, the microbial agent prepared by mixing Mortierella alpina, Trichoderma asperellum and Mucor circinelloides according to a certain ratio is firstly used, can be used in the complex and changing environment, and can provide effects in the poor soil environment, wherein the functional microbes provide high tolerance to heavy metals, and can maintain activity and perform efficient repair in the heavily heavy metal polluted soil.

Description

Technical field [0001] The invention relates to a microbial agent for treating soil heavy metal pollution, and a preparation method and application thereof, belonging to the technical field of soil treatment. Background technique [0002] The problem of ecological destruction has become a key issue restricting human development. Dumping sites, tailings ponds and surrounding polluted sites formed by mining activities often have complex pollutants, containing heavy metals and a variety of organic pollutants. These heavy metals have high pollution concentrations and heavy pollution levels, making it difficult for plants and animals to grow in them. , Which seriously affects the ecological environment. Heavy metals in the soil can pollute the surrounding land, waters and atmosphere through surface water runoff and atmospheric dust, and their impact will far exceed the area and space of the waste dump, and the pollution impact will cost a lot of manpower, material and financial resou...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B09C1/10C12N1/14C12R1/885C12R1/785C12R1/645
CPCB09C1/105C12N1/14
Inventor 张旭杨欢欢崔兆杰孙立群张萌萌魏绍山
Owner SHANDONG UNIV
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