Microbial agent for treating heavy metal polluted soil, preparation method and applications thereof
A microbial agent and heavy metal technology, applied in the field of soil remediation, can solve the problems of lack of soil remediation technology, limited application range, difficult growth of animals and plants, etc., and achieve the effect of controlling heavy metal pollution, diverse remediation mechanisms, and increasing the scope of application
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Embodiment 1
[0044] A microbial agent for treating soil pollution by heavy metals, consisting of the following raw materials by weight:
[0045] 80 parts of rice husk, 20 parts of pig manure, 50 parts of chicken manure, 30 parts of soybean meal, 6 parts of single cell protein, 2.5 parts of Mortierella alpina fermentation broth, 2.5 parts of Trichoderma asperellum fermentation broth Parts, 0.5 parts of Mucor circinelloides fermentation broth;
[0046] The total content of bacteria in the microbial agent is 5.3×10 8 cfu / g; the ratio of the number of viable cells of Mortierella alpina (Mortierella alpina), Trichoderma asperellum (Trichoderma asperellum) and Mucor circinelloides (Mucor circinelloides) is 1:1.8:2.5.
[0047] The method for preparing the above-mentioned microbial inoculum for treating heavy metal pollution of soil has the following steps:
[0048] (1) Mortierella alpina was cultivated by primary seed culture, secondary seed culture and expanded culture to obtain 0.4×10 8 cfu / ml Mortiere...
Embodiment 2
[0060] A microbial agent for treating soil pollution by heavy metals, consisting of the following raw materials by weight:
[0061] 50 parts of rice husk, 50 parts of pig manure, 20 parts of chicken manure, 60 parts of soybean meal, 4 parts of single cell protein, 3.5 parts of Mortierella alpina fermentation broth, and 1.5 parts of Trichoderma asperellum fermentation broth 1 part, 1.5 parts of Mucor circinelloides fermentation broth;
[0062] The total content of bacteria in the microbial agent is 8.3×10 8 cfu / g; the ratio of the number of viable cells of Mortierella alpina (Mortierella alpina), Trichoderma asperellum (Trichoderma asperellum) and Mucor circinelloides (Mucor circinelloides) is 1:1:3.6.
[0063] The preparation method is the same as in Example 1.
Embodiment 3
[0065] A microbial agent for treating soil pollution by heavy metals, consisting of the following raw materials by weight:
[0066] 65 parts of rice husk, 35 parts of pig manure, 35 parts of chicken manure, 45 parts of soybean meal, 5 parts of single-cell protein, 3 parts of Mortierella alpina fermentation broth, and Trichoderma asperellum fermentation broth 2 1 part, 1 part of Mucor circinelloides fermentation broth;
[0067] The total content of bacteria in the microbial agent is 6.8×10 8 cfu / g; the ratio of the number of viable cells of Mortierella alpina (Mortierella alpina), Trichoderma asperellum (Trichoderma asperellum) and Mucor circinelloides (Mucor circinelloides) is 1:1.5:3.
[0068] The preparation method is the same as in Example 1.
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