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Application of mucor circinelloides in gossypol degradation

A technology of Mucor circinol and gossypol, which is applied in the application of Mucor circinol to degrade gossypol, microbial strains and its application field, can solve the problems that gossypol has not been reported in relevant literature, and achieve wide application value and cultivation Simple conditions, fast breeding effect

Inactive Publication Date: 2019-04-16
THE INST OF MICROBIOLOGY XINJIANG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no relevant literature report on the degradation of gossypol by Mucor circinarius

Method used

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  • Application of mucor circinelloides in gossypol degradation
  • Application of mucor circinelloides in gossypol degradation
  • Application of mucor circinelloides in gossypol degradation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1: Isolation, screening and identification of Mucor circiniferus CSGW1

[0023] (1) Separation and purification:

[0024] From the cotton straw sample, take 5g sample and add it to 50ml sterile water, mix it, place it on a shaker, shake it at 25°C and a speed of 100rpm / min for 30 minutes, and filter the mixture with a layer of sterile gauze under sterile conditions , discard the filter residue, the filtrate was centrifuged at room temperature at 4000rmp / min for 1 minute, discarded the supernatant, suspended the precipitate with 5ml of sterile water, continued to carry out gradient dilution with sterile water, and then carried out gradient dilution from 10 -6 、10 -7 、10 -8 200ul dilutions were taken from each of the three dilutions, spread on the separation sample plate, and placed in an incubator at 30°C for 5 days. Pick a single colony from the plate, inoculate it on a new separation plate for purification, and repeat 3-4 times to purify Mucor circinelloides...

Embodiment 2

[0038] Embodiment two: Mucor circinelloides degrades gossypol test

[0039] 1. Preparation of seed solution

[0040] (1) Strain cultivation: Select Mucor circinatus CSGW1 strain and inoculate it in liquid PDA medium, culture it in a shaker at 30°C and 120rpm for 3-5 days to the stable stage, and the concentration of the bacteria will contain 15g of dry bacteria per liter body left and right;

[0041] (2) Preparation of liquid seeds: Take 5ml of the cultured bacterial solution, centrifuge at 8000rpm for 5 minutes at room temperature, discard the supernatant, suspend the precipitate with 5ml of sterile water, centrifuge again, and repeat the centrifugation for 3 times, then use 500ul of sterile water to dissolve The sediment is suspended, and the bacterial solution is the liquid seed.

[0042] 2. Gossypol degradation test: Put 8ml of liquid separation medium in a 2×18cm test tube, insert 500ul of the liquid seeds prepared in step 2 under sterile conditions, place it in a shake...

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Abstract

The invention provides application of mucor circinelloides in gossypol degradation. Aiming at the technical status that in the prior art, there are no records about the application of the mucor circinelloides in the gossypol degradation, and the current status that cottonseed meal is used as the second largest plant protein resource of China, and in order to provide high-degradation-rate and stable-fermentation-property strains for the biological detoxification technology of cottonseed meal, the application of the mucor circinelloides in the gossypol degradation is provided. The mucor circinelloides has the advantages of being high in gossypol degradation rate, simple in culture condition and high in reproduction speed, the gossypol degradation rate is 67.5%, the cottonseed meal is detoxified by using the degradation ability of the strains to gossypol, and the application has a wide application value in the technical field of microbial strain application.

Description

technical field [0001] The invention relates to the technical field of microbial strains and applications thereof, in particular to the technical field of the application of a Mucor circinolus that degrades gossypol. Background technique [0002] Gossypol (Gossypol), commonly known as gossypol, accounts for 0.7%-4.8% of ordinary cottonseed. It is a polyphenol dinaphthalene derivative produced by the pigment glands of Malvaceae plants, and exists in the pigment glands of roots, stems, leaves and seeds of cotton In the body, especially in the globular gland of the seed cotton kernel, the content is more. Pure gossypol is yellow crystal, soluble in low boiling petroleum ether and water, melting point 181-185°C, molecular weight 518.55. There are two forms of gossypol in cottonseed, namely bound gossypol and free gossypol. Conjugated gossypol is not absorbed in the digestive system of the body, and can be quickly excreted with feces, with low toxicity. The active groups in th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L5/20C12N1/14C12R1/785
CPCA23L5/28C12N1/145C12R2001/785
Inventor 王志方秦新政杨新平陈竞王小武代金平冯蕾古丽努尔·艾合买提
Owner THE INST OF MICROBIOLOGY XINJIANG ACADEMY OF AGRI SCI
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