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Aspergillus niger strain and application thereof

A technology of Aspergillus niger and tobacco black shank, which is applied in the fields of application, fungicides, fungi, etc., can solve the problems of reduced antagonism and poor growth ability of strains, and achieve the effect of preventing and treating tobacco black shank

Inactive Publication Date: 2012-10-17
ZHENGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The document of CN200910233578 discloses an organic fertilizer made of antagonistic bacteria, and the bacterial strain and the organic fertilizer are applied to the soil together, which is beneficial to the colonization of the bacterial strain, but after the fertilizer is used, as a foreign bacterium, the growth ability of the bacterial strain becomes poor, Antagonism will be reduced

Method used

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  • Aspergillus niger strain and application thereof
  • Aspergillus niger strain and application thereof
  • Aspergillus niger strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Example 1 Isolation and screening of antagonistic bacteria against tobacco black shank

[0052] 1) Isolation of pathogenic bacteria

[0053] Tobacco black shank was isolated from infected tobacco plants collected in Luoshan County of Xinyang and Wuyang County of Luohe. When the cotton-like mycelium grows around the cigarette block, a little mycelium is picked and purified, and inserted into the PDA slant medium for subsequent use. PDA medium: 200 g potato, 20 g sucrose, 15-20 g agar, 1000 ml distilled water, natural pH, pressure 1.05 kg / cm 2 , Sterilize at 121.3°C for 20 min.

[0054] The micrographs show that the hyphae of the fungus are colorless and without septa; cyst peduncles protrude from the stomata of the diseased tissue, and 1-3 roots are bundled; the sporangia are terminal or lateral, pear-shaped to oval, with papillae, The sporangia can release 5-30 zoospores; the zoospores are nearly round or reniform, colorless, with two lateral flagella.

[0055] 2) I...

Embodiment 2

[0064] Example 2 Morphological observation of Ty-3

[0065] The initial colony of Ty-3 bacteria is flat and white, with sparse hyphae, and the mature colony is black and colorless on the back. The hyphae are colorless and clearly separated, with branches, 3-4 μm in diameter. When mature, the hyphae grow conidiophores, the main trunk and the top of each side branch are stalked, the stalks are in the shape of spindle rods with two pointed ends, and the conidia are spherical or oval like Figure 8 , Figure 9 shown.

Embodiment 3

[0066] Example 3 Observation of Ty-3 Parasitic Phenomena

[0067] In the confrontation culture between Ty-3 bacteria and black shank pathogenic bacteria, it was found that Ty-3 bacteria grew vigorously, and could produce a large number of short fluffy aerial hyphae and black conidia clusters, and the junction of black shank mycelia and Ty-3 bacteria An obvious antagonistic band appeared, with a width of 12-15.4 mm. Ty-3 bacteria can cross the colony junction and directly occupy the growth space of the black shank colony, and can grow on the bacteria, produce spores, and gradually digest the bacteria. Figure 10 , Figure 11 , Figure 12 shown. The antagonism of the bacterium is manifested as the formation of a bacteriostatic zone or a bacteriostatic zone, which significantly inhibits the growth of pathogenic mycelia, and has obvious nutrient competition and secretion of anti-biological substances to produce antagonism.

[0068] Observing the hyphae at the junction of the c...

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Abstract

The invention discloses a strain of Aspergillus niger Ty-3 and application thereof. The strain has been deposited in the China Center for Type Culture Collection on July 8, 2011 with the accession number of CCTCC NO.: M 2011241. The mycelia of the strain can adhere to and wind around the mycelia of tobacco black shank and parasitize on the mycelia of tobacco black shank and grow; through long time parasitic growth, the mycelia of the strain can kill and decompose pathogens, thereby exerting a strong inhibitory effect on the growth of pathogens. The Aspergillus niger strain is applicable to preparation of bactericides used for controlling tobacco diseases.

Description

technical field [0001] The invention relates to a microbial strain and its application, in particular to an Aspergillus niger strain and its application in the prevention and treatment of tobacco black shank. [0002] Background technique [0003] Tobacco black shank is an important soil-borne disease of tobacco, which usually infects tobacco roots, causes diseases of crop roots and even whole plants, and causes significant economic losses. At present, agricultural measures such as disease-resistant varieties and crop rotation are mainly used in production to prevent and control tobacco soil-borne diseases. The Kunming Institute of Botany, Chinese Academy of Sciences has developed varieties resistant to black shank (patent number CN01108714.5), but due to the diversity of disease-resistant genes required for disease-resistant varieties, and the influence of the environment is relatively large, the control effect is not ideal; crop rotation is also One of the effective meth...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/04A01P3/00C12R1/685
Inventor 王岩李红丽郭夏丽李玉红
Owner ZHENGZHOU UNIV
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