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Cladosporium cladosporioides and application thereof

A technology of Cladosporium cladosporium and strains, applied in the application, fungi, pesticides and other directions, can solve the problems of increasing pesticide residues, polluting the environment, endangering human health and life, etc. Rapid growth and good production traits

Inactive Publication Date: 2016-03-16
CITRUS RES INST OF CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The use of chemical pesticides increases the amount of pesticide residues in agricultural products, seriously pollutes the environment, and endangers human health and life.

Method used

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  • Cladosporium cladosporioides and application thereof
  • Cladosporium cladosporioides and application thereof
  • Cladosporium cladosporioides and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The isolation of embodiment 1 bacterial strain

[0027] Disinfect the body surface of the collected naturally infected Panonychus citrus with 70% alcohol, put it in a 25°C incubator for 3 to 5 days, and make fresh spores grow on the surface of the insect; under sterile conditions, Use an inoculation loop to pick a small amount of fresh spores and streak them on a PDA medium plate containing 100 μg / ml ampicillin; turn the streaked culture dish over and place it in a 25°C incubator for cultivation; pick out the grown spores after 3 to 4 days For typical colonies without miscellaneous bacteria, use an inoculation needle to pick up a small piece of culture medium with hyphae at the edge of the colony, transfer it to the center of the culture medium on the slant of the test tube, and culture at a constant temperature of 25°C for 7 days.

Embodiment 2

[0028] The identification of embodiment 2 bacterial strains

[0029] (1), morphological identification

[0030] The isolated CQBBW3 strain was inoculated on a PDA plate and cultured at 25°C to observe the morphological characteristics of the colony. The results showed that the colony of the CQBBW3 strain growing on the PDA plate was round and raised, grayish white, with a yellow outer ring; the hyphae had separation and branching ; Spores are oval, single cell, colorless. see figure 1 , 2 .

[0031] (2), ITS sequence amplification, sequence determination and molecular identification of CQBBW3 strain

[0032] Using ITS1 and ITS4 as primers (see Table 1), a sequence of about 600 bp was amplified from the genomic DNA of the strain, and the sequence obtained after the PCR product was sequenced was compared with the existing nucleic acid sequence in GenBank by Blast. The results showed that, The rDNA-ITS sequence sequencing result of the strain is shown in SEQ ID No. 1, and th...

Embodiment 3

[0035] Determination of the insecticidal activity of embodiment 3 CQBBW3 bacterial strains to citrus Panonychus

[0036] Scrape the CQBBW3 mycelium grown on the PDA plate for 7 days and dissolve it in 0.02% Tween sterile water, shake and filter to collect the spore suspension, and configure 6 spore concentration gradients: 1.7×10 3 pcs / mL, 1.7×10 4 pcs / mL, 1.7×10 5 pcs / mL, 1.7×10 6 pcs / mL, 1.7×10 7 pcs / mL, 1.7×10 8 Individuals / mL; 10mL of each concentration of spore suspension was evenly sprayed on 5 leaves, and 50 heads of Panonychus citrus were connected on each leaf. The control group was sprayed with 10mL sterile water containing 0.02% Tween-80, and the deaths were counted every day Rate. See Tables 2 and 3 for the results of the insecticidal activity of the CQBBW3 strain against Panonychus citrus. LC of Cladosporium cladodes against Panonychus citrus 50 4.66×10 8 spores / mL, LT 50 It is 6.2353 days.

[0037] Table 2 Regression equation of toxicity of CQBBW3 strai...

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Abstract

Provided is cladosporium cladosporioides CQBBW3. The preservation number is CGMCC No.11439. The homology between the rDNA-ITS sequence of the cladosporium cladosporioides and the corresponding sequence of the cladosporium cladosporioides reaches up to 95%. The cladosporium cladosporioides CQBBW3 which is directly separated and screened from naturally-infected citrus red mites is high in conidium-producing ability, the conidium-producing quantity reaches 4.3*10<8> conidia / mL on the fourteenth day, the production character is good, growth is fast, and the colony diameter is 6.6125 cm on the fourteenth day. Meanwhile, the citrus red mite killing activity is high, the citrus red mite killing effect is good, LC50 of female adult mites of citrus red mites is 4.66*10<8> conidia / mL, and LT50 of the female adult mites of the citrus red mites is 6.2353 days. An insecticidal fungus preparation produced through the cladosporium cladosporioides effectively controls the colony quantity of the citrus red mites.

Description

technical field [0001] The invention relates to a new microbial strain, in particular to an insecticidal Cladosporium strain and application thereof, belonging to the field of biological pesticides. technical background [0002] Since the invention of chemical pesticides, chemical pesticides have grown at an astonishing rate of 5% per year. Although in the past few decades, chemical pesticides have played an important role in agriculture as an increase in production and pest control, the extensive use of chemical pesticides has brought about serious "3R" problems (i.e. resistance to pesticides, residence of pesticide residues, No matter how rampant Resurgence is, it has also brought a series of ecological and environmental problems. The long-term and extensive use of chemical pesticides has led to an increase in pesticide-resistant pests, especially in the past 10 years, the resistance of cotton bollworms, aphids, diamondback moths, Spodoptera litura, and spider mites to py...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/04A01P7/02A01P7/04A01N59/20A01N43/54A01N37/50A01N47/38A01N43/713A01N37/42A01N53/08
Inventor 胡军华姚廷山王娟刘浩强李鸿筠冉春
Owner CITRUS RES INST OF CHINESE ACAD OF AGRI SCI
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