Wuyiencin spore powder and production technology thereof

A Wuyi fungus spore and production process technology, applied in the field of biological pesticides, can solve the problems of difficult breakthrough development of biochemical metabolite technology, disease prevention and control, and increase the concentration of use, so as to achieve high content of effective antibacterial substances and spore reproduction Ability to improve and avoid interference effects

Inactive Publication Date: 2013-06-05
潍坊万胜生物农药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the promotion and use of biopesticides, some weaknesses have also been found: one is the high production cost, which brings about high cost of use; In the process of preventing and treating crop diseases, it will also promote the resistance of diseases and insect pests, and the use concentration must be increased, which has affected the actual promotion; the fourth is that the main market of water-based biochemical pesticides is spraying on greenhouse vegetables in protected areas, irrigating roots, adding The higher the temperature, the lower the effect, and the prevention and control of diseases will also be affected.

Method used

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  • Wuyiencin spore powder and production technology thereof
  • Wuyiencin spore powder and production technology thereof
  • Wuyiencin spore powder and production technology thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A production process of Wuyiencin spore powder, comprising the following steps:

[0027] 1. Put the activated slanted seeds of Wuyi bacteria into the liquid fermentation medium at a mass percentage of 8%, keep the temperature at 28°C, culture on a shaking table, and ferment for 3 days.

[0028] Among them, the composition of the liquid fermentation medium used is: 180 grams of potatoes, 16 grams of glucose, 1.5 grams of potassium dihydrogen phosphate, 1 gram of magnesium sulfate, 0.5 grams of yeast extract, 0.5 grams of peptone, 0.5 grams of sodium chloride, 1000 milliliters of water, pH 6.5.

[0029] 2. Mix the mycelium of Wuyi bacteria cultivated in the liquid fermentation medium with the solid fermentation medium at a weight ratio of 1:3, stir evenly (the standard is that it can disperse after being held in a ball), at 28°C, Solid-phase fermentation is carried out under the condition of 60% humidity. Under the condition of solid-state fermentation, the thickness of ...

Embodiment 2

[0042]A production process of Wuyiencin spore powder, comprising the following steps:

[0043] 1. Put the activated slanted seeds of Wuyi bacteria into the liquid fermentation medium at a mass percentage of 5%, keep the temperature at 28°C, culture on a shaking table, and ferment for 2 days.

[0044] Among them, the composition of the liquid fermentation medium used is: 200 grams of potatoes, 15 grams of glucose, 2 grams of potassium dihydrogen phosphate, 0.5 grams of magnesium sulfate, 0.5 grams of yeast extract, 0.5 grams of peptone, 0.5 grams of sodium chloride, 1000 milliliters of water, pH 6.5.

[0045] 2. Mix the mycelium of Wuyi bacteria cultivated in the liquid fermentation medium with the solid fermentation medium at a weight ratio of 1:3, stir evenly (the standard is that it can disperse after being held in a ball), and put it at 30°C, The solid-phase fermentation is carried out under the condition of 70% humidity. Under the condition of solid-state fermentation, th...

Embodiment 3

[0057] A production process of Wuyiencin spore powder, comprising the following steps:

[0058] 1. Put the activated slanted seeds of Wuyi bacteria into the liquid fermentation medium at a mass percentage of 7%, keep the temperature at 28°C, culture on a shaking table, and ferment for 3 days.

[0059] Among them, the composition of the liquid fermentation medium used is: 200 grams of potatoes, 15 grams of glucose, 1 gram of potassium dihydrogen phosphate, 1 gram of magnesium sulfate, 0.5 grams of yeast extract, 0.5 grams of peptone, 0.5 grams of sodium chloride, 1000 milliliters of water, pH 7.5.

[0060] 2. Mix the mycelium of Wuyi bacteria cultured in the liquid fermentation medium with the solid fermentation medium in a weight ratio of 1:4, and stir evenly (the standard is that it can be dispersed after being held in a ball), and the 30℃ , The solid-phase fermentation is carried out under the condition of 80% humidity. Under the condition of solid-state fermentation, the t...

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Abstract

The invention discloses wuyiencin spore powder and a production technology thereof. The production technology comprises liquid culture, solid culture, drying, crushing and separation so as to obtain the wuyiencin spore powder, wherein spore number of the wuyiencin spore powder is greater than or equal to 50.0*10<8>, the water content is less than or equal to 8%, the pH value is 4.0-7.0, and the resolution ratio of the effective components is less than or equal to 5%. In the invention, through the expansion culture of liquid phase before solid phase, the spore powder content of the obtained wuyiencin spore powder is greater than 50*10<8> / g, thus the stress resistance of the spores is strong, thereby being favorable for storage and transportation; the wuyiencin spore powder can be used in various modes by directly spraying powder or spraying after being prepared into a liquid preparation, thereby being convenient to use; and the wuyiencin spore powder is particularly suitable for preventing diseases of woods and fruit trees, and has an obvious prevention effect.

Description

technical field [0001] The invention relates to wuyiencin, specifically a wuyiencin spore powder and a production process thereof. The wuyiencin spore is used as a biological pesticide to prevent and control plant diseases and insect pests, and belongs to the field of biological pesticides. Background technique [0002] Most of the traditional fungicides are chemical pesticides, and some biological pesticides use submerged fermentation to make the metabolites produced by beneficial microorganisms have bactericidal and antibacterial effects (also known as biochemical products). Traditional chemical pesticides and fungicides, whose active ingredients are difficult to decompose in nature, have a long residual period, and are harmful to organisms. Although they are not advocated, they are still the main products in plant protection technology. [0003] Most of the biochemical pesticides have low toxicity and high environmental compatibility, and are currently advocated technolog...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N3/00A01N63/00A01P3/00C12R1/465
Inventor 孙波李鹏雷音李树青
Owner 潍坊万胜生物农药有限公司
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