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Nomuraea rileyi strain, and application thereof

A technology of Nomura bacteria and strains, applied in application, fungi, biocides, etc., can solve the problem of lack of high insecticidal activity, and achieve the effects of good production traits, strong sporulation ability and strong insecticidal activity

Active Publication Date: 2012-06-20
CHONGQING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there have been no relevant reports on the use of entomogeneous fungi to simultaneously control insect pests such as rice leaf roller and rice stem borer. Borer entomopathogenic fungal strains

Method used

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  • Nomuraea rileyi strain, and application thereof
  • Nomuraea rileyi strain, and application thereof
  • Nomuraea rileyi strain, and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Example 1 Isolation of bacterial strains

[0017] Disinfect the surface of the collected naturally-infected rice leaf roller worms with 70% alcohol, put them in a 25°C incubator for 3-5 days, and make fresh spores grow on the surface of the insects; under sterile conditions, Use an inoculation loop to pick a small amount of fresh spores and streak them on a Sabouraud malt agar (SMAY) medium plate containing 100 μg / ml ampicillin; turn the streaked culture dish over and place it in a 25°C incubator for cultivation; 3— After 6 days, select the typical colony without miscellaneous bacteria, pick up a small piece of culture medium with hyphae at the edge of the colony, transfer it to the center of the culture medium on the slant of the test tube, and cultivate it at a constant temperature of 25°C for 15 days.

Embodiment 2

[0018] Embodiment 2 strain identification

[0019] (1) Morphological identification

[0020] The isolated CQNr126 strain was inoculated on Sabouraud Malt Agar (SMAY) plates, and cultured at 25°C to observe the morphological characteristics of the colonies, the sporulation structure, and the shape and size of the spores. The results showed that the colonies grew slowly and densely on the malt juice agar. The colonies were light green at the beginning of the culture, then dark green and colorless on the back. Aerial hyphae smooth with septa, 2-3 μm wide. Conidiophores are upright, separated, produced from substrate hyphae, 150-180 μm long, 2-2.5 μm wide, 3-4 wheel-shaped branch clusters, 2-3 phialides are closely attached to the branches, smooth , transparent to green. Bottle stem 4.7-6.5 × 2.5-3μm, mostly short columnar ( figure 1 a). The conidia are broadly oval, smooth, grass green in piles, and the size is 3.5-4.5 × 2-3 μm ( figure 1 b). Judging from the above c...

Embodiment 3

[0024] Example 3 Indoor insecticidal activity of CQNr126 against rice leaf roller and rice stem borer

[0025] Collect mature spores of CQNr126 cultured on Sabouraud Malt Agar (SMAY) plates, disperse them with 0.05% (W / V) Tween-80, and make a final concentration of 1×10 8 spores / ml suspension. The larvae of rice leaf roller and rice stem borer were spot-dropped, and each test worm was dripped with 2 μl of medicine with a micro-spotter, and 30 larvae were inoculated. After inoculation, they were reared under the conditions of room temperature 25°C and humidity 50-70%, set 5 repeated, with water as the blank control. Record the number of dead insects, calculate the survival rate, and obtain the fungal LT by statistical analysis of DPS2000 software 50 (when lethal).

[0026] Table 1 Insecticidal activity of CQ Nr126 strain against rice leaf roller and rice stem borer larvae indoors

[0027]

[0028] 5-7 days after inoculation, the larvae of rice leaf roller and Chilo sup...

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PUM

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Abstract

The invention relates to a Nomuraea rileyi strain CQNr126, with a preserving number of CGMCC No.4610. The strain provided by the present invention has a strong sporulation capability. On a 3 / 4 SMAY medium plate, the amount of spores reaches 2.2*10<8> spore / cm<3>. The strains have a good productive performance, grow fast, and have a strong insecticidal activity and a good insecticidal effect against rice leaf rollers and chilo suppressalis. Under the effects of the strains, the median lethal time (LT50) of rice leaf rollers and chilo suppressalis are respectively 6.03 days and 6.42 days. With a fungal preparation prepared from the strains, rice leaf roller population and chilo suppressalis population can be effectively controlled.

Description

Technical field [0001] The invention involves a pesticide fungus, which specifically involves a Laishino strain and its application, which belongs to the field of biological pesticide. Background technique [0002] Insect fungus is the most important insecticidal microbial group. Under natural conditions, about 70%of insect diseases are caused by fungi. They are the only pathogenic microorganisms with touch characteristics and epidemic.It is used in the microorganisms of pest control, but no resistance to pests is found, which has great potential for solving the problem of drug resistance of pests.At the same time, insecticidal fungi has strong vertical and horizontal diffusion capabilities, which helps the continuous control of pests and the occurrence of pest epidemic diseases, which is particularly beneficial to the control of the migrant pest of rice longitudinal rolls. [0003] Rice longitudinal rolls and erythrodes are one of the main pests of rice. They are widely distribu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01N63/04A01P7/04C12R1/645
Inventor 夏玉先彭国雄曾德玉
Owner CHONGQING UNIV
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