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Beauveria bassiana, use thereof in preventing and treating tea tree leaf-eating beetles and fungus agent

A technology of Beauveria bassiana and a fungicide, which is applied in the application, insecticide, biocide and other directions, can solve problems such as destroying the ecological environment and endangering human health, and achieves fast growth speed, strong sporulation ability, and pathogenicity. strong effect

Pending Publication Date: 2021-04-09
TEA RES INST OF FUJIAN ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The agricultural 3R problems caused by the long-term and large-scale use of chemical pesticides seriously damage the ecological environment and endanger human health

Method used

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  • Beauveria bassiana, use thereof in preventing and treating tea tree leaf-eating beetles and fungus agent
  • Beauveria bassiana, use thereof in preventing and treating tea tree leaf-eating beetles and fungus agent
  • Beauveria bassiana, use thereof in preventing and treating tea tree leaf-eating beetles and fungus agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Beauveria bassiana provided by the invention, Latin formal name used at school: Beauveria bassiana (Bals.) Vuill, named after FJBb1338, has the following characteristics: the bacterial strain is collected from the tea garden of the Tea Research Institute of Fujian Academy of Agricultural Sciences, Shekou Town, Fuan City, Fujian Province It was isolated from the dead body of the weevil weevil, grown on Sabouraud medium (SDAY) at 25±1°C for 5 days, and the diameter of the colony was 17.83 mm, and reached 51.5 mm in 15 days. The colony is white to milky white, initially felt-like, and later forms a milk powder-like spore layer, which is thick and uniform. Branched hyphae, regular, colorless and smooth, sometimes growing in clusters; sporogenous cells (bottle stems) densely clustered on short and enlarged stalk cells, bottle-shaped, zigzag-shaped upper part of sporogenous cells, and tapering at the top long. Conidia are spherical or subspherical, unicellular, transparent, ...

Embodiment 2

[0031]Example 2 Isolation and identification of Beauveria bassiana FJBb1338 of the present invention

[0032] 1. Isolation of Beauveria bassiana FJBb1338

[0033] The strains to be tested were collected from the experimental tea garden of the Tea Research Institute of Fujian Academy of Agricultural Sciences, Shekou Town, Fu'an City, Fujian Province, and the corpses of the adult weevils, which died of natural diseases, were collected from the experimental tea garden. The surface of the corpses was disinfected with 5% sodium hypochlorite solution, and then The insect corpses were cut into small pieces with a scalpel, and inoculated into Dogutine oat selective medium to separate and purify, and the pure culture of the obtained strain was named Beauveria bassiana FJBb1338.

[0034] 2. Identification of Beauveria bassiana FJBb1338

[0035] (1) Morphological identification

[0036] On the SDAY medium, the colony of Beauveria bassiana FJBb1338 was white felt, and a white spore laye...

Embodiment 3

[0042] Example 3 Strain growth rate, spore production and spore germination rate of Beauveria bassiana FJBb1338 of the present invention

[0043] 0.2mL spore suspension (1.0×10 6 Spores / mL) were evenly spread on the SDAY plate and cultivated for 5 days, then punched out fresh bacterial mass from the above plate with a hole puncher with a diameter of 6mm, inoculated on the SDAY plate, and cultured at 25°C under light conditions of 12L / 12D , repeated 3 times. The colony diameters of the 5th, 10th and 15th day of culture were measured by the straightedge cross method. The measurement results showed that the colony diameter of Beauveria bassiana strain FJBb1338 cultured on the SDAY plate for 15 days could reach 51.5 mm (Table 1). Use a hole puncher with a diameter of 6mm to randomly pick a bacterial block from the center of the colony cultivated on the SDAY plate for 14 days to the edge 1 / 2, put it into a triangular flask (50mL) containing 20mL germination solution, and use a ma...

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Abstract

The invention belongs to the technical field of biological prevention and control of agriculture and forestry pests, and particularly relates to beauveria bassiana, use thereof in preventing and treating tea tree leaf-eating beetles and a fungus agent. The beauveria bassiana is named as FJBb1338 and has the preservation number of CGMCC No.20253. The invention provides the use of the beauveria bassiana in preventing and treating the tea tree leaf-eating beetles. The invention further provides thefungus agent for preventing and treating the tea tree leaf-eating beetles. Beneficial effects are as follows: the beauveria bassiana FJBb1338 has a high growth speed and a strong sporulation capacity and the fungus agent of the beauveria bassiana can be remarkably reduce the number of the leaf-eating beetles in a tea garden. Meanwhile, the strain can be stored in the tea garden for a long time, has high secondary infection capacity and is suitable for biological prevention and treatment of the leaf-eating beetles in the tea garden.

Description

technical field [0001] The invention belongs to the technical field of biological control of agricultural and forestry pests, and in particular relates to a Beauveria bassiana and its application and bacterial agent in preventing and treating tea tree leaf-eating beetles. Background technique [0002] Tea tree leaf-eating beetles are a class of Coleoptera pests that feed on tea tree buds and leaves (such as tea weevil, tea big gray weevil, tea aphid thick-legged weevil and tea horn breast beetle, etc.). Distributed in major tea-producing regions in my country, and often breaks out in local tea gardens. This type of pest occurs one generation a year, mainly through adults biting the leaves of tea trees, causing nicks or holes, which seriously affect the yield and quality of tea. Among them, the tea weevil and the tea weevil not only damage tea, but also damage camellia, camellia, citrus, pear, peach and so on. [0003] For a long time, in addition to agricultural control and...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/30A01P7/04C12R1/645
CPCA01N63/30
Inventor 王定锋李良德李兆群吴光远
Owner TEA RES INST OF FUJIAN ACADEMY OF AGRI SCI
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