56 results about "PORCINE REPRODUCTIVE RESPIRATORY SYNDROME VIRUS" patented technology

The invention discloses a virulent strain HuN4 for porcine reproductive and respiratory syndrome, and an attenuated vaccine strain HuN4-F112 (the collection number of microorganism: CGMCC No.2484) attenuated because of virulent strain HuN4 passage, and also discloses the application of the attenuated vaccine strain in preventing or curing the highly pathogenic blue-ear disease, which belongs to the biomedical field. The attenuated vaccine strain HuN4-F112 can be prepared into single-vaccine or combined strain (live vaccine or inactivated vaccine), can effectively prevent or cure the highly pathogenic blue-ear disease, and also can be prepared into diagnostic reagent for the highly pathogenic blue-ear disease diagnosis. The attenuated vaccine strain HuN4-F112 of the invention has the advantages of good security and high protection efficiency.

The invention discloses a medicament for treating highly pathogenic porcine respiratory and reproductive syndrome and a method for preparing the same. The medicament is mainly prepared from rhubarb, dyers woad leaf, dandelion, southernwood, forsythia, gardenia, groundsel, plaster stone, scutellaria and astragalus. The preparation method comprises the following steps: according to a ratio, all materials are weighed up, the rhubarb is ground into powder, and soaked in boiling water for 30 minutes to form a leach solution, the 9 materials of dyers woad leaf, dandelion, southernwood, forsythia, gardenia, groundsel, plaster stone, scutellaria and astragalus are mixed for decoction and pentad temperature, the obtained liquid is mixed with the leach solution obtained after the rhubarb powder is soaked to prepare the medicament. The invention has the advantages of reasonable and unique formula, simple preparation process, low cost, little toxic and side effect and good control efficiency.

The invention relates to preparation and application of a pig blue ear disease (porcine reproductive and respiratory syndrome, PRRSV) protein engineering vaccine. The vaccine uses body fluid and cellular immune epitopes of the main structural proteins GP5 and minor structural proteins GP3 and GP4 of PRRSV as the vaccine frame structure, the proteins are connected by a flexible linker, then put into series connection with the Thelper epitope, cloned into a pRSETB vector, transformed into Escherichia coli, and subjected to fermentation, purification, and emulsification to obtain the recombinant PRRS protein the engineering vaccine with ideal immunogenicity. The invention also relates to the usage method of the vaccine. The animal experiment shows that the PRRS protein engineering vaccine has virus attack protecting force equal to that of an attenuated vaccine and higher than that of an inactivated vaccine, can produce rapid and effective immune response, and prevent porcine reproductive and respiratory syndrome virus infection.

The present invention discloses a feed additive and an injection of a yolk antibody resisting the blue-ear disease in pig and a preparation method thereof. The feed additive of the yolk antibody resisting the blue-ear disease in pig is extracted and prepared by the collected yolk antibody of the immunized laying hens which are immunized by a bacterin for the blue-ear disease in pig. The injection of the yolk antibody resisting the blue-ear disease in pig is extracted and prepared by the collected yolk antibody of the immunized laying hens which are immunized by a bacterin for the blue-ear disease in pig. The injection of the yolk antibody resisting the blue-ear disease in pig prevents the blue-ear disease in pig by improving the antibody level of a pig organism to the blue-ear disease in pig. The present invention has beneficial effects that the feed additive of the yolk antibody resisting the blue-ear disease in pig is convenient for feeding and is very suitable for pig. The injection of the yolk antibody resisting the blue-ear disease in pig is used after the pig catches the disease, which has faster medicinal effect. The feed additive and a injection of a yolk antibody resisting the blue-ear disease in pig and the preparation method thereof has obvious curative effect, low cost, fast acting, no toxic and side effect, no drug-resistant strain which can be produced, simple preparation, wide raw material source, convenient use and easy popularization and can not produce drug-resistant strain.

The invention discloses a traditional Chinese drug composition for pig and a preparation method and application thereof. The composition comprises drug substances of 6-18 parts of indigofera tinctoria by weight, 4-12 parts of Chinese amphibious knotweed rhizome by weight and 5-15 parts of Chinese mosla herb by weight. The preparation method of the composition comprises: distilling Chinese mosla herb, and collecting the distillate; adding indigofera tinctoria and Chinese amphibious knotweed rhizome in the residue and residual liquid after distillation and decocting for 2-3 times, mixing the decoctive solution and concentrating the solution to viscous liquid, adjusting an alcohol concentration of ethanol to be 60 percent to 70 percent, precipitating, filtrating and concentrating the liquor, adding ethanol and adjusting the alcohol concentration to be 75 percent to 80 percent, sequentially performing precipitation, filtration and concentration of filter liquor, and adding distilled water and refrigerating for precipitation, filtration, and concentration; and blending the distillate of Chinese mosla herb with the collected concentrated solution of indigofera tinctoria and Chinese amphibious knotweed rhizome. The Chinese drug composition can be used for preparation of traditional Chinese veterinary drug to treat blue ear pig disease, porcine circovirus infection, pseudo rabies, piglet pullorum disease of piglet, paratyphoid of piglet, pig erysipelas and edema disease of piglet. Moreover, the Chinese drug composition has the advantages of few side effect and safe and convenient use.

The invention provides a swine influenza virus-porcine reproductive and respiratory syndrome virus mixed virus-like particle. The swine influenza virus-porcine reproductive and respiratory syndrome virus mixed virus-like particle contains a swine influenza virus stromatin M1, a swine influenza virus surface antigen hemagglutinin HA and an envelope fusion protein, wherein the envelope fusion protein contains an extramembranous domain of a main epitope of a porcine reproductive and respiratory syndrome virus protein GP5, and a transmembrane domain and an intramembranous domain of an influenza virus hemagglutinin HA; the extramembranous domain of the main epitope of the porcine reproductive and respiratory syndrome virus protein GP5 replaces an extramembranous domain of an end 5' of the swine influenza virus surface antigen hemagglutinin HA, and the length of the extramembranous domain of the main epitope of the porcine reproductive and respiratory syndrome virus protein GP5 is approximately equal to that of the replaced extramembranous domain; and the swine influenza virus surface antigen hemagglutinin HA and the porcine reproductive and respiratory syndrome virus protein GP5 are expressed on the surface of the swine influenza virus-porcine reproductive and respiratory syndrome virus mixed virus-like particle simultaneously. The invention also provides a bivalent vaccine for preventing swine influenza and blue ear disease. The bivalent vaccine contains the swine influenza virus-porcine reproductive and respiratory syndrome virus mixed virus-like particles and adjuvants. The invention also provides a preparation method of the swine influenza virus-porcine reproductive and respiratory syndrome virus mixed virus-like particle.

The invention relates to a gene recombined swine cholera salmonella choleraesuis vaccine for a blue-ear disease and application thereof, belongs to the technical field of animal bacteria genetic engineering, and particularly relates to construction of recombined swine cholera salmonella choloraesuis without resistance maker and used for expressing main immunogenicity membrane protein of porcine reproductive and respiratory syndrome virus(PRRSV) and preparation and application of a live vaccine. Recombined swine cholera salmonella choleraesuis C501-GP5m expressing the swine PRRSV GP5m protein is gained and preserved in the China model culture preservation centre, and the preservation number is CCTCC NO: M2012275. The invention further discloses a method for preparing swine cholera salmonella choleraesuis and the porcine reproductive and respiratory syndrome virus vaccine from the recombination strain and application thereof. The prepared recombined live vaccine can stimulate swine to develop the immunity to resist swine cholera salmonella choloraesuis and PRRSV main immunogenicity membrane protein GP5m, and can effectively prevent the infection of paratyphus suum and PRRSV.

The invention discloses a loop-mediated isothermal amplification quick detection technology for a highly pathogenic blue ear disease. By using a special primer and utilizing a special region of a loop-mediated isothermal amplification technology (RT-LAMP) platform amplification target sequence, a highly pathogenic blue ear virus nucleic acid is detected from molecular level with the assistance of a series of quality control, negative contrast and positive contrast. The method has the characteristics of simplicity, convenience, economization, quickness, sensitivity and specificity, and has broad application prospect.

The invention discloses the application of MARCO in screening blue ear disease resistant pigs. A study on that invention shows that the expression amount of MARCO in the pig body is closely related tothe infection of PRRSV, the expression of MARCO is inhibited after PRRSV is infected with PAMs, the interference of MARCO promotes PRRSV replication, and the overexpression of MARCO inhibits virus replication; when the expression of MARCO is abnormally high in vivo, the pigs are resistant to PRRSV, otherwise the pigs are susceptible to PRRSV. Therefore, MARCO can be used as a new criterion to judge the susceptibility or resistance of PRRSV. This study laid a solid foundation for identifying susceptibility to PRRSV and screening and breeding of PRRSV-resistant pigs, has good clinical application value for screening and breeding of PRRSV-resistant pigs, has important significance for prevention and control of blue-ear disease of pigs, and is also conducive to breeding excellent strain of pigs.

The invention discloses a traditional Chinese medicine composition for pigs and a preparation method and application of the traditional Chinese medicine composition for pigs. The composition is prepared from, by weight 6-18 parts of Fructus Broussonetiae, 15-45 parts of Schizomussaenda Dehiscens, 9-27 parts of Radix Mahoniae and 8-24 parts of Rhapis Excelsa. The preparation method includes: mixing the four traditional Chinese medicines, adding water, soaking, decocting for 2-3 times, combining decoctions, concentrating to a thick state, using ethyl alcohol to adjust the alcohol concentrate to 60%-70%, precipitating, filtering, adding ethyl alcohol to concentrated medicine liquid to adjust the alcohol concentration to 75%-80%, precipitating, filtering, concentrating filtrate, adding distilled water, refrigerating, precipitating, filtering, and concentrating to obtain a required dosage form. The traditional Chinese medicine composition is used for preparation of Chinese veterinary drugs for treating blue ear disease, porcine circovirus disease, pseudorabies, necrotic enteritis, swine enzootic pneumonia and haemophilus parasuis and is free of evident toxic and side effects and safe and convenient to use.

The invention relates to a preparation method and an application for a pig blue ear disease (porcine reproductive and respiratory syndrome virus, PRRSV) recombinant ubiquitin vaccine. The preparation method comprises the following steps: by taking a body fluid which contains PRRSV major structural proteins GP5, GP6 and GP7 and a cellular immune epitope as a vaccine framework structure, connecting the vaccine framework structure through a flexible linker and then connecting the vaccine framework structure with ubiquitin in series, cloning into a pRSETA vector and then transforming escherichia coli, performing processes such as fermenting, purifying and emulsifying, thereby obtaining the recombinant blue ear disease protein engineering vaccine with ideal immunogenicity. The invention further relates to a using method of the vaccine. According to the animal experiments, the toxic substance counteracting protection of the blue ear disease protein engineering vaccine is equivalent to that of an attenuated vaccine, and higher than that of an inactivated vaccine, so that T lymphocyte multiplication immune reaction on cellular level can be generated by stimulating, antibody immune reaction with virus neutralizing activity is produced on body fluid level, and the pig blue ear disease can be prevented.

The invention discloses a natural and effective transfer factor for controlling the pig blue-ear disease. By using the transfer factor, the pig blue-ear disease can be prevented and controlled, and normal body cells can be protected from being harmed by pig blue-ear disease viruses, therefore, the incidence rate is reduced; and meanwhile, pigs infected by the pig blue-ear disease viruses are treated by using the transfer factor, the clinical symptom can be effectively improved, the cure rate is improved and the death rate is reduced. The preparation method for the transfer factor comprises the following steps: preparing viral antigen, extracting the viral antigen and the viral antigen immunized pigs, extracting the transfer factor of the pig blue-ear disease from the spleens of the immunized pigs, and the like.