56 results about "PORCINE REPRODUCTIVE RESPIRATORY SYNDROME VIRUS" patented technology

The invention discloses a virulent strain HuN4 for porcine reproductive and respiratory syndrome, and an attenuated vaccine strain HuN4-F112 (the collection number of microorganism: CGMCC No.2484) attenuated because of virulent strain HuN4 passage, and also discloses the application of the attenuated vaccine strain in preventing or curing the highly pathogenic blue-ear disease, which belongs to the biomedical field. The attenuated vaccine strain HuN4-F112 can be prepared into single-vaccine or combined strain (live vaccine or inactivated vaccine), can effectively prevent or cure the highly pathogenic blue-ear disease, and also can be prepared into diagnostic reagent for the highly pathogenic blue-ear disease diagnosis. The attenuated vaccine strain HuN4-F112 of the invention has the advantages of good security and high protection efficiency.

The application relates to a domesticated porcine reproductive and respiratory syndrome virus low virulent strain (JXA1-R strain), immunogenicity substance containing the viral strain, and a porcine reproductive and respiratory syndrome virus low virulent live vaccine developed through making use of the strain. The live vaccine is used to prevent the porcine reproductive and respiratory syndrome,in particular the highly pathogenic blue-ear porcine disease.

The invention provides compositions and methods for the detection and quantification of PRRSV antibodies and antibody fragments using polypeptides.

Provided are porcine proteins, which are interferons except for interferon-alpha. The proteins can prevent or treat porcine reproductive and respiratory syndrome in vivo.

The invention discloses strong and weak strains of NADC30-like porcine PRRS and applications thereof. The virulent NADC30-like porcine PRRS virus strain of the present invention is named as NADC30-like porcine PRRS virus SD strain, and its microorganism preservation number is: CGMCC NO.19691. By attenuating the SD strain, the present invention has also obtained a NADC30-like attenuated strain of porcine PRRS virus, named after the NADC30-like porcine PRRS virus SD-R strain, and its microorganism preservation number is: CGMCC NO.19692 . Experiments have proved that the SD strain of the present invention has the characteristics of strong virulence and stable pathogenicity to piglets, and can be used for testing, preventing or treating porcine reproductive and respiratory syndrome of class NADC30; the attenuated vaccine strain SD-R strain of the present invention has safety High and good protective effect, it can be used to prevent NADC30-like porcine reproductive and respiratory syndrome. Therefore, the SD strain of the present invention and its attenuated vaccine SD-R strain can be used to prepare a single vaccine or combined vaccine (live or inactivated vaccine) for preventing and treating NADC30 porcine reproductive and respiratory syndrome, and can also be prepared as a diagnostic NADC30 vaccine. Diagnostic reagents for porcine reproductive and respiratory syndrome.

The invention discloses application of chlorine dioxide to prevention and control of a porcine reproductive and respiratory syndrome, and in particular relates to application of high-purity chlorine dioxide liquid to preparation of a medicine for resisting a PRRSV (Porcine Reproductive and Respiratory Syndrome Virus) and / or preventing and controlling the porcine reproductive and respiratory syndrome. According to the application disclosed by the invention, experiments of each level and each phase are performed, thus proving that the chlorine dioxide still has a remarkable anti-virus effect on the porcine reproductive and respiratory syndrome virus, especially a high-pathogenicity strain HP-PRRSV for the first time, and a foundation is laid for researching a novel medicine for resisting the porcine reproductive and respiratory syndrome. The used chlorine dioxide is the high-purity chlorine dioxide liquid, is safe and non-toxic, has no mutagenic, teratogenic or carcinogenic effects and can be orally taken; the chlorine dioxide has no irritation and toxin to organisms and does not cause medicine residues, so that the chlorine dioxide is hopefully used as a novel bioactive molecule for preventing and controlling the porcine reproductive and respiratory syndrome; a prerequisite for researching and developing an anti-virus medicine for the porcine reproductive and respiratory syndrome virus by utilizing the chlorine dioxide is provided, and the chlorine dioxide has a very good application prospect in the aspects of prevention and control of the porcine reproductive and respiratory syndrome.

The invention discloses application of an HPSE gene in screening of pigs with resistance to the reproductive and respiratory syndrome, and provides a group of HPSE gene expressive quantity detection primers. The primers comprise an upstream primer HPSE-F and a downstream primer HPSE-R, and respectively have sequences as shown in the SEQ ID NO.1-2. Researches indicate that the HPSE gene expressive quantity in a big body is closely related to PRRSV infection, the HPSE gene coded heparanase is used for regulating PRRSV release and infection efficiency by regulating the expressive quantity of heparan sulfate on a cytomembrane; when the HPSE expressive quantity is low, a pig has resistance to PRRSV, and is easily affected otherwise. HPSE gene can be used as a novel standard for determining the susceptibility or resistance to PRRSV, and lays a solid foundation for screening and culture of PRRSV-resistance pigs, and has an important meaning for prevention and control of porcine reproductive and respiratory syndrome.