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Yolk antibody feed additive and injection for resisting porcine reproductive and respiratory syndrome and preparation thereof

A technology for feed additives and pig PRRS, which is applied in antiviral agents, antibodies, animal feed, etc., can solve the problems that the preparation method of yolk antibody for pig PRRS has not been reported, and achieve obvious prevention and treatment of pig PRRS, with effective results Fast, Visible Results

Inactive Publication Date: 2008-09-10
TIANJIN SHENGJI GRP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] At present, there is no report about the preparation method of pig yolk antibody against PRRS

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Preparation method of egg yolk antibody preparation against pig blue ear disease

[0023] The commercially available PRRS vaccine was used to pass basic immunization and booster immunization, and the non-immune laying hens were immunized with the PRRS vaccine in the first round, and the immune dose was 2 n-1 The gradient increases, and the interval between each round is 10 days. 10 days after the end of the third round of immunization, the immunized eggs are collected after the titer of egg yolk antibody is measured, and the egg yolk is aseptically obtained, diluted with 0.01M sterile phosphate buffer solution, 0.5% hypromellose and phthalates were treated, the filtrate was spray-dried, and the powder was packaged and preserved.

Embodiment 2

[0025] Preparation method of egg yolk antibody injection against pig blue ear disease

[0026] Immunize the non-immune laying hens with the porcine PRRS vaccine, and measure the HI titer of the immune eggs with the hemagglutination inhibition test after the basic immunization and booster immunization. After reaching the standard, harvest the yolk and dilute it with sterile distilled water, and acidify it with lactic acid solution. Treat and remove impurity proteins, pump the filtrate for salting out, adjust the concentration of IgY after desalting, and then package it to prepare anti-PRRS egg yolk antibody injection. The injection contains egg yolk antibody against porcine blue-ear disease with effective titer ≥ 1:64. The injection can be prepared for treating pig blue-ear disease. The yield of IgY is over 95%, and one immunized egg can generally obtain 80-110 mg of IgY.

Embodiment 3

[0028] The preparation method of the feed additive of the yolk antibody against pig blue ear disease

[0029] The preparation method comprises preparing the immunogen of porcine PRRS virus; immunizing the immunogen to healthy laying hens, and the injection dose of the immunogen is 0.5-3.0ml / chicken; collecting the eggs laid by the hen after 8-10 days of immunization, Keep chicken eggs containing the yolk antibody titer of not less than 1:64 against PRRS for later use; dilute with 0.01M sterile phosphate buffer solution, 0.5% hydroxypropylmethylcellulose, phthalic acid For salt treatment, the above-mentioned liquid is taken for pasteurization, spray-dried in a conventional spray dryer to obtain a feed additive. The feed additive contains egg yolk antibody against porcine blue-ear disease with an effective ratio of ≥1:64. The feed additive can be prepared for preventing and treating pig blue-ear disease.

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Abstract

The present invention discloses a feed additive and an injection of a yolk antibody resisting the blue-ear disease in pig and a preparation method thereof. The feed additive of the yolk antibody resisting the blue-ear disease in pig is extracted and prepared by the collected yolk antibody of the immunized laying hens which are immunized by a bacterin for the blue-ear disease in pig. The injection of the yolk antibody resisting the blue-ear disease in pig is extracted and prepared by the collected yolk antibody of the immunized laying hens which are immunized by a bacterin for the blue-ear disease in pig. The injection of the yolk antibody resisting the blue-ear disease in pig prevents the blue-ear disease in pig by improving the antibody level of a pig organism to the blue-ear disease in pig. The present invention has beneficial effects that the feed additive of the yolk antibody resisting the blue-ear disease in pig is convenient for feeding and is very suitable for pig. The injection of the yolk antibody resisting the blue-ear disease in pig is used after the pig catches the disease, which has faster medicinal effect. The feed additive and a injection of a yolk antibody resisting the blue-ear disease in pig and the preparation method thereof has obvious curative effect, low cost, fast acting, no toxic and side effect, no drug-resistant strain which can be produced, simple preparation, wide raw material source, convenient use and easy popularization and can not produce drug-resistant strain.

Description

technical field [0001] The invention belongs to the field of feed additives and medicines, and in particular relates to a feed additive and injection against pig yolk antibody against PRRS and a preparation method thereof. Background technique [0002] At present, porcine viral diseases are still the main infectious diseases that seriously restrict the healthy development of the pig industry. Viral infectious diseases occur in hundreds of millions of pigs in different seasons every year in the country. Once the pig is infected, it will have a sudden onset, severe clinical symptoms, easy to spread, and high mortality and mortality, which is extremely harmful to the breeding industry. [0003] PRRS is caused by the infection of pig herds by PRRS virus. Pig PRRS is relatively common and very serious. However, judging from the current incidence characteristics of PRRS, the incidence has changed from reproductive disorders in sows in the past to respiratory syndrome in nursery ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/42A61K9/08A23K1/00C07K16/10A61P31/14A23K20/147
Inventor 古长庆
Owner TIANJIN SHENGJI GRP CO LTD
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