Transfer factor against swine blue ear disease

A transfer factor and porcine blue-ear disease technology, which is applied in the field of transfer factor against porcine blue-ear disease, can solve the problems of weak drug competition, limited curative effect, and inability to prevent and reduce repeated freezing and thawing time. Accurate curative effect and long-lasting effect

Inactive Publication Date: 2009-08-05
TIANJIN RINGPU BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0013] Although there are some anti-PRRS clinical medicines on the market, the existing medicines are not competitive and have limite...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Embodiment 1: Preparation of anti-pig PRRS transfer factor

[0043] 1. Inoculate porcine PRRS virus VR2332 (Genbank accession No. 217415) or LV (Genbank accession No. 96262) in the allantoic fluid of 9-11-day-old chicken embryos and culture at 37°C for 48 hours. Take chicken embryo allantoic fluid, freeze and thaw repeatedly 5 times at -20°C, centrifuge at 5000rpm for 30min, discard the precipitate and collect the supernatant;

[0044] 2. Take the supernatant, and use the sucrose density gradient centrifugation to purify the supernatant to extract the virus. The sucrose concentration is 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, and centrifuged at 150000rpm for 4 hours , determine the position of the centrifuge tube where the porcine PRRS virus is located according to the molecular weight of the porcine PRRS virus, and suck it out for subsequent use;

[0045] 3. The pigs immunized with the obtained porcine PRRS virus antigen were selected as healthy immunized pigs, and th...

Embodiment 2

[0055] Example 2: Preparation of anti-PRRS transfer factor

[0056] 1. Inoculate porcine PRRS virus in the allantoic fluid of 9-11 day old chicken embryos and culture at 37°C for 72h. Take chicken embryo allantoic fluid, freeze and thaw repeatedly 5 times at -20°C, centrifuge at 5000rpm for 30min, discard the precipitate and collect the supernatant;

[0057] 2. Get the supernatant, and the supernatant is purified by sucrose density gradient centrifugation to extract the virus. The sucrose concentration is 15%, 30%, 45%, 60%, 75%, centrifuged at 180000rpm for 3 hours, and determined according to the molecular weight of porcine PRRS virus The position of the centrifuge tube where the porcine PRRS virus is located is sucked out for later use;

[0058] 3. The pigs immunized with the obtained porcine PRRS virus antigen were selected as healthy immunized pigs, and the above-mentioned extracted virus antigens were subcutaneously injected into the immunized pigs at multiple points, a...

Embodiment 3

[0068] Example 3: Detection of the anti-PRRS transfer factor prepared by the process of the present invention

[0069] The anti-pig PRRS transfer factor prepared by the process described in Examples 1 and 2 is detected as follows:

[0070] 1. Ultraviolet spectrophotometric measurement: the sample obtained above has an absorption peak at 252±2nm, and ABS 260 / ABS 280 >2.0.

[0071] 2. The standard solution is light yellow and the pH value is between 6.0 and 8.0.

[0072] 3. Bacteriological examination: there is no need for aerobic, anaerobic, saprophytic bacteria and fungi in the samples obtained above.

[0073] 4. Detection of 20% sulfosalicylic acid: the sample obtained above has no turbidity and precipitation, indicating that the protein reaction is negative, and it does not contain macromolecular protein.

[0074] 5. Determination of nucleic acid content: the nucleic acid content of the samples obtained above was determined by the orcinol method to be 615.08 μg / mL and 5...

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PUM

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Abstract

The invention discloses a natural and effective transfer factor for controlling the pig blue-ear disease. By using the transfer factor, the pig blue-ear disease can be prevented and controlled, and normal body cells can be protected from being harmed by pig blue-ear disease viruses, therefore, the incidence rate is reduced; and meanwhile, pigs infected by the pig blue-ear disease viruses are treated by using the transfer factor, the clinical symptom can be effectively improved, the cure rate is improved and the death rate is reduced. The preparation method for the transfer factor comprises the following steps: preparing viral antigen, extracting the viral antigen and the viral antigen immunized pigs, extracting the transfer factor of the pig blue-ear disease from the spleens of the immunized pigs, and the like.

Description

technical field [0001] The invention relates to the field of medicines and preparations thereof, and more specifically relates to a transfer factor against pig PRRS. Background technique [0002] Pig blue-ear disease is characterized by reproductive disorders (abortion, stillbirth, mummified fetus) of pregnant sows and respiratory diseases of pigs of various ages, especially piglets, and has now become one of the main diseases in large-scale pig farms. [0003] The disease was once known as "Mystery Pig Disease", "New Pig Disease", "Porcine Epidemic Abortion and Respiratory Syndrome", "Porcine Reproductive and Respiratory Syndrome", "PRRS", "Pig PRRS", etc. , my country lists it as a second-class infectious disease. [0004] Porcine reproductive and respiratory syndrome virus is a single-stranded positive-sense RNA virus, belonging to the order Mandoviridae, the family Arteriviridae, and the genus Arterivirus. It does not agglutinate mammalian or avian red blood cells, has ...

Claims

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Application Information

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IPC IPC(8): C07K1/34C07K1/14
Inventor 管于平苏建东李旭东
Owner TIANJIN RINGPU BIO TECH
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