Method for detecting high-pathogenicity blue ear disease

Abstract

The invention discloses a loop-mediated isothermal amplification quick detection technology for a highly pathogenic blue ear disease. By using a special primer and utilizing a special region of a loop-mediated isothermal amplification technology (RT-LAMP) platform amplification target sequence, a highly pathogenic blue ear virus nucleic acid is detected from molecular level with the assistance of a series of quality control, negative contrast and positive contrast. The method has the characteristics of simplicity, convenience, economization, quickness, sensitivity and specificity, and has broad application prospect.

Description

technical field [0001] The invention belongs to a detection technology, specifically a method for detecting whether pigs suffer from highly pathogenic PRRS. Background technique [0002] Highly pathogenic porcine blue-ear disease is an acute and highly lethal disease caused by a mutant strain of porcine reproductive and respiratory syndrome virus. The morbidity rate of piglets can reach 100%, the mortality rate can reach more than 50%, the abortion rate of sows can reach more than 30%, and the fattening pigs can also fall ill and die. The pathological features are that infarcts appear on the edge or surface of the spleen, and hemorrhagic infarction can be seen under the microscope; the kidneys are earthy yellow in color, with large bleeding spots from needle tips to small grains on the surface, and bleeding spots can be seen in the subcutaneous, tonsil, heart, bladder, liver and intestinal tract and bleeding spots. Renal interstitial inflammation, heart, liver and bladder ...

AI Technical Summary

Problems solved by technology

[0006] In the prior art, the ELISA method or RT-PCR method is mostly used to detect highly pathogenic PRRS in pigs, but these methods take a long time to detect, require high equipment conditions for detection, and relatively low detection sensitivity

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