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Virus velogen strain for porcine reproductive and respiratory syndrome, attenuated vaccine strain thereof and application thereof

A technology for respiratory syndrome and attenuated vaccines, which is applied in the invention and also relates to the field of application of attenuated vaccine strains in the prevention or treatment of highly pathogenic porcine PRRS, achieving good safety effects

Active Publication Date: 2010-10-13
HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It can be seen that although there are many kinds of PRRS vaccines at present, none of them can produce enough protective effect on pigs

Method used

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  • Virus velogen strain for porcine reproductive and respiratory syndrome, attenuated vaccine strain thereof and application thereof
  • Virus velogen strain for porcine reproductive and respiratory syndrome, attenuated vaccine strain thereof and application thereof
  • Virus velogen strain for porcine reproductive and respiratory syndrome, attenuated vaccine strain thereof and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1 Isolation and identification of porcine reproductive and respiratory syndrome virus virulent strain HuN4

[0032] 1. Materials and methods

[0033] 1.1Marc-145 cells, monoclonal antibodies against PRRSV-N and GP5 protein are preserved by Harbin Veterinary Research Institute.

[0034] 1.2 Collection and processing of disease materials Anticoagulant blood, lungs, kidneys and lymph nodes of pigs with high fever in a pig farm in a southern city were collected.

[0035] 1.3 Marc-145 cell culture Take out 2-3 ampoules of frozen Marc-145 cells from liquid nitrogen, and thaw quickly in a 37°C water bath. Centrifuge each ampoule at 1000r / min for 5-10min, discard the supernatant, and use 2-3ml of DMEM containing 100IU / ml penicillin, 80μg / ml streptomycin, 6% calf serum and 0.5% hydrolyzed milk protein Resuspend the cells, then inhale into a culture flask containing 10ml of culture medium, shake well, and store at 37°C, 5% CO 2 After 72 hours, the culture was expanded ...

Embodiment 2

[0069] (3) The molecular characteristics of the PRRSV HuN4 strain are highly consistent with other newly isolated strains, but there are significant differences with the previous strains, especially the deletion of the Nsp2 gene and the mutation of the structural protein gene sequence. Which of these changes affect the virulence? Whether the change plays a decisive role remains to be further studied. To sum up, the PRRSV isolates isolated from the so-called "high fever syndrome" pig farms in 2006 had two deletions in the Nsp2 gene. Molecular epidemiological analysis found that the pig farms in different provinces The popular PRRSVs are highly homologous to each other, and are quite different from previous PRRSVs, especially the pathogenicity is greatly enhanced. Therefore, it can be preliminarily identified that this new PRRSV variant is the main cause of the epidemic of "high fever syndrome" in southern pigs. Etiology (maybe some pig farms are caused by other reasons). Embod...

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Abstract

The invention discloses a virulent strain HuN4 for porcine reproductive and respiratory syndrome, and an attenuated vaccine strain HuN4-F112 (the collection number of microorganism: CGMCC No.2484) attenuated because of virulent strain HuN4 passage, and also discloses the application of the attenuated vaccine strain in preventing or curing the highly pathogenic blue-ear disease, which belongs to the biomedical field. The attenuated vaccine strain HuN4-F112 can be prepared into single-vaccine or combined strain (live vaccine or inactivated vaccine), can effectively prevent or cure the highly pathogenic blue-ear disease, and also can be prepared into diagnostic reagent for the highly pathogenic blue-ear disease diagnosis. The attenuated vaccine strain HuN4-F112 of the invention has the advantages of good security and high protection efficiency.

Description

technical field [0001] The invention relates to a virulent strain of virus and an attenuated vaccine strain attenuated by it, in particular to a virulent strain of porcine reproductive and respiratory syndrome virus HuN4, and an attenuated vaccine strain HuN4 attenuated by the virulent strain HuN4 - F112, the present invention also relates to the application of the attenuated vaccine strain in the prevention or treatment of highly pathogenic pig PRRS, which belongs to the field of biomedicine. Background technique [0002] In the spring and summer of 2006, pig "high fever disease" characterized by fever, skin redness, high morbidity and high mortality occurred in some parts of southern my country, and then gradually spread to most provinces in the country. A large number of pathogens were isolated and identified, and animal experiments confirmed that the main pathogen of pig "high fever" is a mutant strain of highly pathogenic porcine blue-ear disease virus, which is also kn...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N7/00C12N7/08A61K39/12A61P31/14C12Q1/70
Inventor 童光志田志军周艳君安同庆彭金美
Owner HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
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