Applications of radix salviae miltiorrhizae in prevention or preparation of drugs for treating porcine reproductive respiratory syndrome virus infection

A technology for respiratory syndrome and virus infection, applied in the field of medicine, can solve the problems of difficulty in taking effect of synthetic drugs, difficulty in PRRSV, and few antiviral western medicines, and achieves the effects of scientific and reasonable preparation method, inhibition of infection and strong operability

Inactive Publication Date: 2019-11-12
武汉百药联科科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This directly leads to the difficulty of using vaccines to control PRRSV
[0006] Because the virus is strictly intracellular parasitic, it relies on many functions of the host cell to replicate and proliferate, which makes it difficult for synthetic drugs (western medicines) to play a role
In order to ensure the effectiveness of humans using antiviral drugs such as Virin (Molinguanidine), Ribavirin, Amantadine, and Rimantadine to prevent and control influenza, the U.S. FDA and the Ministry of Agriculture of the People's Republic of China have all banned such drugs. The production, operation and use of antiviral veterinary drugs, so there are few antiviral western medicines that can be used clinically and the effect is not good

Method used

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  • Applications of radix salviae miltiorrhizae in prevention or preparation of drugs for treating porcine reproductive respiratory syndrome virus infection
  • Applications of radix salviae miltiorrhizae in prevention or preparation of drugs for treating porcine reproductive respiratory syndrome virus infection
  • Applications of radix salviae miltiorrhizae in prevention or preparation of drugs for treating porcine reproductive respiratory syndrome virus infection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The preparation of embodiment 1 salvia miltiorrhiza extract

[0028] 1. Experimental materials:

[0029] Absolute ethanol was purchased from Sinopharm Chemical Reagent Co., Ltd.;

[0030] 2. Experimental steps:

[0031] 1) Weigh 20g of Salvia miltiorrhiza, crush it into dry powder; add 10 times the volume of absolute ethanol to soak for 12h;

[0032] 2) Heating 200ml of Salvia Miltiorrhiza and the soaking solution to reflux for 2 hours, and recovering the filtrate;

[0033] 3) Add 200ml of absolute ethanol to the salvia miltiorrhiza residue, heat and reflux for a second time, and recover the filtrate;

[0034] 4) The filtrates heated to reflux for two times were mixed and rotated to evaporate until the powder of Salvia miltiorrhiza extract was obtained.

Embodiment 2

[0035] Example 2 The toxicity test of Salvia miltiorrhiza extract to Marc-145 cells

[0036] 1. Experimental materials:

[0037] 1) Marc-145 cells (monkey embryo kidney epithelial cells) are susceptible cells to PRRSV. Therefore, in this experiment, the toxicity of Salvia miltiorrhiza extract on Marc-145 cells was tested first, and different concentrations of Salvia miltiorrhiza In Marc-145 cells, to determine the maximum concentration of the salvia miltiorrhiza extract when the cell survival rate reaches more than 90%, to provide reference data for subsequent inhibition experiments.

[0038] 2) PRRSV strain: CH-1a strain;

[0039] 3) Fetal bovine serum was purchased from Zhejiang Tianhang Biotechnology Co., Ltd., and DMEM was purchased from Hyclone Company.

[0040] 4) Broad-spectrum antiviral drug—ribavirin was purchased from Selleck Company.

[0041] 2. The experimental steps are as follows:

[0042] 1) Inoculation of Marc-145 cells: use DMEM medium containing 10% fetal...

Embodiment 3

[0050] Example 3 The inhibition rate detection of Salvia miltiorrhiza extract to PRRSV

[0051] 1. Experimental materials:

[0052] 1) Marc-145 cells (monkey embryo kidney epithelial cells) are susceptible cells to PRRSV.

[0053] 2) PRRSV strain: CH-1a strain

[0054] 3) Fetal bovine serum was purchased from Zhejiang Tianhang Biotechnology Co., Ltd., and DMEM was purchased from Hyclone Company.

[0055] 4) Ribavirin, a broad-spectrum antiviral drug, was purchased from Selleck Company.

[0056] 2. The experimental steps are as follows:

[0057] 1) Inoculation of Marc-145 cells: use DMEM medium containing 10% fetal bovine serum to configure the cells into a cell suspension, inoculate 10,000 cells per well into a 96-well cell culture plate, and inoculate a volume of 100 ul per well;

[0058] 2) Culture of Marc-145 cells: place the inoculated Marc-145 cells at 37°C, 5% CO 2 Cultivate in the incubator for 24h;

[0059] 3) Add Danshen extract or ribavirin and carry out virus ...

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Abstract

The invention provides a new purpose of radix salviae miltiorrhizae in the technical field of medicines, and specifically relates to applications of radix salviae miltiorrhizae in the prevention or preparation of drugs for treating porcine reproductive respiratory syndrome virus infection. A radix salviae miltiorrhizae extract product can effectively inhibit the infection of a porcine reproductiverespiratory syndrome virus on a Marc-145 cell within a non-toxic dose range, namely, the inhibition rate of the radix salviae miltiorrhizae extract product on the porcine reproductive respiratory syndrome virus can reach more than 90% when the concentration of the radix salviae miltiorrhizae extract product is 0.004-0.032 mg / mL after being dissolved in dimethyl sulfoxide, and under this concentration, the survival rate of the Marc-145 cell is more than 90%. Thus, the radix salviae miltiorrhizae can be further developed into drugs for treating and preventing the porcine reproductive respiratory syndrome virus.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to the application of salvia miltiorrhiza in the preparation of medicine for treating porcine reproductive and respiratory syndrome virus infection. Background technique [0002] Porcine reproductive and respiratory syndrome, also known as "pig blue ear disease" and "porcine mystery disease", is a highly contagious infectious disease of pigs caused by porcine reproductive and respiratory syndrome virus (Porcine reproductive respiratory syndrome virus, PRRSV) . Since it was discovered in 1990, the disease has rapidly become one of the most serious viral diseases in the pig industry, seriously restricting the development of the global pig industry. [0003] Under natural conditions, pigs of different breeds and ages can be infected with PRRSV, but pregnant sows and piglets less than 1 month old are most susceptible to infection, and fattening pigs will also develop the disease after...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/537A61P31/14
CPCA61K36/537A61K2236/33A61K2236/39A61P31/14
Inventor 张红雨吕博敏张青叶黄清
Owner 武汉百药联科科技有限公司
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