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Porcine interferon gene family and application thereof in blue-ear porcine disease resistant medicament and vaccine adjuvant

An interferon and gene technology, applied in the field of genetic engineering, can solve the problems of low protection effect of immunized pigs, poor immunogenicity of inactivated vaccines, etc., and achieve the effects of facilitating gene insertion and screening, enhancing immune protection effect, and convenient preparation.

Inactive Publication Date: 2013-07-31
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

So far, various inactivated vaccines have been studied and prepared at home and abroad, but existing reports show that the immunogenicity of inactivated vaccines is poor, and the protective effect on immunized pigs is very low

Method used

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  • Porcine interferon gene family and application thereof in blue-ear porcine disease resistant medicament and vaccine adjuvant
  • Porcine interferon gene family and application thereof in blue-ear porcine disease resistant medicament and vaccine adjuvant
  • Porcine interferon gene family and application thereof in blue-ear porcine disease resistant medicament and vaccine adjuvant

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Embodiment Construction

[0032] Hereinafter, the present invention will be further specifically described by taking the pig PRRS drug and vaccine adjuvant involved in the present invention to be used alone and in combination with pig PRRS inactivated vaccine against highly pathogenic PRRS Jiangxi A1 strain of pigs as an example.

[0033] The construction process of porcine novel interferon plasmid in drug and adjuvant. Obtain a domestic pig liver sample and extract its genome, and obtain a complete gene of a new type of interferon in domestic pigs including a signal peptide of 22 amino acids and a total length of 171 amino acids by PCR method, and its nucleotide sequence is SEQ ID NO.2, The encoded amino acid sequence is SEQ ID NO.1. The above genes were inserted into the prokaryotic cloning vector pMD-19T (product of TaKaRa Company), and transformed into Escherichia coli DH5-α, positive clones were selected, and the correct genes were confirmed to be obtained after sequencing. The above gene was inser...

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Abstract

Provided are porcine proteins, which are interferons except for interferon-alpha. The proteins can prevent or treat porcine reproductive and respiratory syndrome in vivo.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, and specifically relates to a new porcine interferon gene family and its application as anti-pig PRRS drug and vaccine adjuvant. Background technique [0002] There are several types of porcine type I interferon, and each type has a group of multi-gene families, which are cytokine families with antiviral effects. Under normal physiological conditions, they are not expressed or have a small amount of constitutive expression in animals. When the body is infected by a virus, they not only have antiviral effects, but also can up-regulate the body's immune response to clear the virus. Studies have shown that type I interferon can also activate B cells and help B cells to quickly produce antibodies against pathogens; promote the expression of MHC-I antigens in most cells, induce the proliferation of NK cells and T cells and increase their cytotoxic activity; Helper CD8 + The growth of me...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/20C12N15/63C07K14/555A61K48/00A61K39/39A61P31/14A61P31/16A61P31/20
CPCA61K38/00C07K14/555A61P31/14A61P31/16A61P31/20
Inventor 郑兆鑫赵欣程功刘明秋严维耀
Owner FUDAN UNIV
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