402results about How to "Good clinical value" patented technology

The invention belongs to the field of pharmaceutical agents, relates to a polymer micelle lyophilized agent encapsulating an insoluble antitumor drug as well as a preparation method and an application thereof. The polymer micelle lyophilized agent is prepared by carrying out molecular self-assembly on a methoxy poly(ethylene glycol) 2000-polyester block copolymer to form micelles, and then encapsulating the insoluble antitumor drug in a hydrophobic core formed by the polyester. The lyophilized agent has high encapsulation rate, high drug loading and small particle size, can significantly improve the water solubility of the insoluble drug and result in passive targeting of more antitumor drugs to concentrate in the tumor tissues, thus improving an anti-tumor treatment effect and reducing the toxic and side effects of drugs, and can be used to prepare the drugs used for the treatment of lung cancer, intestinal cancer, mammary cancer, ovarian cancer, etc. The lyophilized agent can also be quickly dissolved and dispersed to form a transparent micellar solution after water for injection, normal saline solution and the like are added, and is used for the preparation of the drugs for treating primary intestinal cell carcinoma.

The invention relates to a photosensitive PRP (platelet-rich plasma) gel and a preparation method and application thereof. The preparation method comprises the following steps of mixing a biocompatible medium solution of a macromolecule modified by an o-nitrobenzyl photo response group and an extracted PRP according to a certain ratio, so as to form a gel precursor solution; then, radiating the gel precursor solution by light, and enabling the o-nitrobenzyl group in the macromolecule modified by the o-nitrobenzyl photo response group to generate photochemical reaction under the excitation action of a light source, so as to produce an aldehyde functional group; generating coupling reaction with amino distributed at the surface of the protein in the PRP to form an imine bond, so as to realize the preparation of the photosensitive PRP gel. Compared with the prior art, the photosensitive PRP gel prepared by the method has the advantages that the internal cell factors can be slowly released, and the tight and seamless bonding between the PRP gel and wounds is realized.

The invention provides a glomerulus-targeted protein nanoparticle pharmaceutical composition and an application thereof. The protein nanoparticle pharmaceutical composition is mainly prepared from a protein ingredient, a pharmacological active substance and a stabilizer, and the grain size of the protein nanoparticle pharmaceutical composition ranges from 10nm to 170nm. With the application of the pharmaceutical composition, passive targeted drug delivery of glomerular mesangial cells is achieved, and the aggregation concentration of a drug in a glomerulus is obviously improved or the aggregation duration of the drug in the glomerulus is prolonged, so that the curative effect of the drug is remarkably improved, and meanwhile, the toxic and side effects of the drug on a non-targeted part are greatly reduced.

The invention discloses a hepatocellular carcinoma automatic grading method based on an SE-DenseNet deep learning framework and an enhanced MR image. The method comprises the following steps of 1) collecting data; 2) preprocessing all hepatocellular carcinoma three-dimensional images with enhanced MR; 3) enhancing the training data; 4) based on the enhanced training data, training a hepatocellularcarcinoma grading prediction model, namely an SE-DenseNet network; and 5) carrying out hierarchical prediction on the test data by adopting the trained model, and evaluating the classification performance of the hepatocellular carcinoma hierarchical prediction model. According to the automatic pathological grading method for the hepatocellular carcinoma multi-modal enhanced MR image which is composed of the steps of image preprocessing, the image enhancement, the hepatocellular carcinoma multi-modal enhanced MR image classification, SE-DenseNet network training and SE-DenseNet network testing, the hepatocellular carcinoma automatic grading can be realized, and the problems of manpower consumption, time consumption and subjective difference existing in manual hepatocellular carcinoma grading can be solved.

A nanopartide and its avidin probe are disclosed. Said gene probe for detecting the mutation or non-mutation of DNA is prepared from nanoparticles or colloidal particles and avidin, streptavidin, or antibiotin through preparing colloidal gold and preparing the compound. Its advantges are high sensitivity, specificity and speed.

The invention provides a hepatic carcinoma targeted photo-thermal therapeutic agent as well as a preparation method and an application thereof and belongs to the field of biomedical engineering. The hepatic carcinoma targeted photo-thermal therapeutic agent is prepared with the method comprising the following steps: (1) preparing biotinylation nanometer microbubbles; (2) preparing a Cy7 fluorescently-labeled biotinylation anti-GPC3 (glypican3) antibody; (3) preparing biotinylation RGO (reduced graphene oxide); (4) coupling the products prepared in the step (1), step (2) and step (3) through a biotin-avidin system to obtain the hepatic carcinoma targeted photo-thermal therapeutic agent. The hepatic carcinoma targeted photo-thermal therapeutic agent has ultrasonic and fluorescence imaging functions, mediates RGO targeted delivery with an ultrasound-targeted microbubble destruction technology, monitors a photo-thermal therapeutic process in real time, can be used for preparing drugs for hepatic carcinoma therapy and has excellent clinical application value.

The invention discloses a biodegradable magnetic nano gold shell microsphere as well as a preparation method and application thereof. The nano gold shell microsphere has a core-shell structure, wherein the inner core is a gelatin magnetic ferroferric oxide microsphere, and the core layer is a gold nano shell; and the diameter of the nano gold shell microsphere is 55-1000 nanometers, the diameter of the inner core is 50-800 nanometers, and the thickness of the shell layer is 5-200 nanometers. The microsphere prepared by the method disclosed by the invention is good in magnetism, narrow in particle size distribution and controllable in outer shell thickness and particle size. Because gelatin is a good in vivo biodegradable material, along with the degradation of the magnetic inner core, the outside nano gold shell layer is disintegrated into gold nano particles and is discharged out of a body at last. The preparation method is simple and convenient in process, simple to operate, low in cost and easy for large-scale production. Meanwhile, the biodegradable magnetic nano gold shell microsphere has functions of magnetic resonance targeted imaging and photo-thermal treatment, and more importantly, the biodegradability of the microsphere greatly reduces the toxic or side effects of the gold shell on human bodies, so that the biodegradable magnetic nano gold shell microsphere has a very good clinical application value.

The invention relates to a preparation method of a tissue model with a cavity structure and the issue model. The method comprises the following steps: as for a target tissue with a cavity structure, acquiring a first three-dimensional geometric model having the same contour shape and size with a tissue; shrinking the first three-dimensional geometric model in the radial direction according to the wall thickness of the tissue, so as to obtain a second three-dimensional geometric model; manufacturing a bracket according to the second three-dimensional geometric model; coating a material used for manufacturing the tissue model on the surface of the bracket according to the wall thickness of the tissue; removing the bracket after the material is solidified, and obtaining the tissue model with the cavity structure. The high-precision tissue model, of which the size is close to that of the real tissue, is obtained for the tissue with the cavity structure through size design; as the tissue model is obtained through coating, the material of the tissue model is not limited by a consumable item of a traditional 3D printer, and the purpose of preparing the tissue model by choosing any proper material is fulfilled.

The present invention provides a method for predicting the risk of occurrence of cancer. It also predicts the presence of BRCA mutations which in turn predicts the risk of developing breast cancer in women. Further, it assesses a cancer patient's level of sensitivity to chemotherapy.

A SARS-Cov gene vaccine based on epitope is configured from the carrier which is a plasmid able to be used for human body and the target antigen which is several B cell epitopes in the extrinsic B protein antigen of human SARS coronavirus through codon optimizing and genetic engineering. Its preparing process is also disclosed.

The invention relates to a functionalized dendrimer-based SPECT-CT bimodal imaging contrast agent and a preparation method thereof. The functionalized dendrimer-based SPECT-CT bimodal imaging contrast agent is obtained by pegylation modified fifth polyamide-amine dendrimer as a polymer carrier material; connecting diethylenetriaminepentaacetic acid on the dendrimer surface through covalent grafting; coating gold nanoparticles through an in-situ synthesis method; carrying out acetylation or hydroxylation on residual surface amino groups; and marking <99m>Tc. The functionalized dendrimer-based SPECT-CT bimodal imaging contrast agent has a very good SPECT/CT imaging effect, realizes SPECT/CT imaging of different in-vivo tissue sites of a mouse, lays good foundation for development of multi-functional tissue specific contrast agent, and has wide application prospects.

The present invention relates to a Chinese medicine tupistra chinensis extract, its preparation method and application. Said extract is obtained by extracting root, stem and leaf of tupistra chinensis, and is a brownish yellow powder. The characteristics of its chemical identification are as follows: Liebermann-Burchard reaction and Molish reaction are positive; the foam test is positive reaction, the foam height of alkaline liquor tube is higher than that of acid liquor tube; Rosen-Heimer reaction at 60deg.C can produce violet red color reaction, it is a steroid saponin compound. Said extract has the action of resisting tumor, resisting inflammation, inhibiting platelet agglutination and resisting thrombosis.

The invention relates to a diagnosis primer and kit for type 2 diabetes, and an application of a non-coding RNA molecular marker. The diagnosis primer comprises a miR-802 forward primer as shown in SEQ ID NO:3 and a miR-802 reverse primer as shown in SEQ ID NO:4; the diagnosis kit for type 2 diabetes comprises the diagnosis primer; the non-coding RNA molecular marker is has-miR-802, or has-miR-802and has-lncRNA-ROIT; and the above diagnosis primer and non-coding RNA molecular marker can diagnose diabetes. The present invention can diagnose type 2 diabetes by utilizing the diagnosis primer/thediagnosis kit and by detecting the non-coding RNA molecular marker. The detection method is simple to operate, high in sensitivity and high in specificity, has small trauma to a subject, can be usedfor diagnosing type 2 diabetes in early stage and different stages, and has obvious clinical application value.

The invention provides lung adenocarcinoma exosome specific miRNAs, and a target gene and applications thereof. High flux sequencing technology is combined with systematic bioinformatics analysis methods, the specific high-content exosome miRNAs related with lung adenocarcinoma is obtained through screening; the RNA sequences of the lung adenocarcinoma exosome specific miRNAs are represented by SEQ ID No.1-3. It is found that the common target gene of the miRNAs is FOXN3. The target gene is capable of inhibiting fibroblast migration capability, reduction of the expression amount of the targetgene is capable of promoting entering of fibroblast into tumor tissues, and promoting tumor growth. The lung adenocarcinoma exosome specific miRNAs and the target gene thereof can be taken as lung adenocarcinoma diagnosis markers and can be used for preparation of diagnostic kits. An inhibitor of the miRNAs or an expression promoter of the target gene can be used for preparing medicines used for treating lung adenocarcinoma, or medication guidance in treatment of lung adenocarcinoma; and the clinical application value is relatively high, and the application prospect is promising.

The invention relates to a preparation method of purified and amplified human mesenchymal stem cells. The method comprises the following steps: (1) collecting mesenchymal stem cells by adopting an antibody-based negative sorting method; and (2) amplifying the mesenchymal stem cells by adopting a serum-free culture medium. The preparation method is superior to a conventional separation method, and the serum-free culture medium provided by the method is remarkably better than a conventional serum culture medium in the fields of safety and amplifying capability and has excellent application prospect.

The invention discloses a zirconium dioxide composite nanometer material with microwave sensitization, chemotherapy dry release and CT imaging functions and a preparation method and application thereof. The zirconium dioxide composite nanometer material comprises a hollow zirconium dioxide nanometer material and trametes polysaccharide. The hollow part of the zirconium dioxide nanometer material of the zirconium dioxide composite nanometer material is filled with the trametes polysaccharide. The grain size of the zirconium dioxide composite nanometer material is 50-500 nm; the thickness of a shell is 10-100 nm. The prepared zirconium dioxide composite nanometer material is good in dispersity; the size and thickness of the shell can be adjusted as needed. The hollow part of the zirconium dioxide is filled with the traditional Chinese medicine trametes polysaccharide with the chemotherapy effect for the first time; the zirconium dioxide composite nanometer material can be applied to the technical fields of microwave sensitization, chemotherapy dry release and CT imaging at the same time, achieves the target of treating the tumor through the diagnosis and treatment integration and targeted hyperthermia chemotherapy and has good clinical application prospects.

The invention relates to a medicament for treating hyperlipidemia, which is a Chinese medicinal preparation. The medicament is a Chinese medicinal compound preparation prepared from cassia seed, turmeric, oriental waterplantain rhizome, notoginseng root and the like which serve as raw materials. A preparation method for the medicament is simple, and is suitable for large-scale industrial production, and a medicament which has a small side effect and an obvious curative effect, and is used for treating the hyperlipidemia and fatty liver is provided clinically.