25results about How to "Good clinical value" patented technology

The invention belongs to the technical field of image processing, and particularly relates to a passive domain adaptive eye fundus image segmentation method based on adaptive mask and curvature regularization, which comprises the following steps: constructing a teacher-student self-training framework, and generating a pseudo tag by using a weak enhancement teacher model to guide student model training, an adaptive mask consistency strategy is introduced in the training process, adaptive mask processing is carried out on a target domain image, the mask proportion and size are adaptively adjusted according to the sample difficulty and the size of a pseudo-label area, a model is guided to keep prediction consistency under the shielding condition, and thus the pseudo-label reliability and the context modeling capability are improved; meanwhile, by introducing an average negative curvature regularization constraint, an irregular boundary in a prediction result is suppressed, the smoothness and structural continuity of a segmentation result are enhanced, and finally the precision and generalization ability of the model in a cross-device and cross-dataset fundus image segmentation task are improved.

The invention relates to a construction method and application of a spontaneous and persistent ventricular tachycardia animal model, in particular to a spontaneous and persistent ventricular tachycardia rat model based on MYL4 gene defect and myocardial infarction induction. The model is constructed by utilizing the synergistic effect of double pathological factors of MYL4 gene conserved gene defects and acquired myocardial infarction, the induction rate of the model is greater than or equal to 90%, the spontaneous duration time reaches 1-3 min, and the method is obviously superior to an existing construction method for inducing the spontaneous and continuous ventricular tachycardia model. The model can simulate the pathophysiological process of ventricular tachycardia after human MYL4 gene related cardiovascular diseases combined with myocardial infarction, provides general technical support for ventricular arrhythmia pathogenesis research, drug screening and medical instrument research and development, and has wide scientific research and clinical transformation value.

This invention relates to the field of biotechnology, and in particular to a primer-probe combination for detecting methylation sites in cancer and its applications. This invention is the first to discover that abnormal methylation at specific sites of the TRIB3 gene is significantly associated with the occurrence and development of colorectal cancer, and abnormal methylation at specific sites of the MAPT gene is significantly associated with the occurrence and development of gastric cancer. This correlation has not been publicly reported in the prior art, possessing novelty and significant clinical application value. The primer-probe combination provided by this invention is used to detect the methylation status of the TRIB3 gene or the MAPT gene in a sample. Specifically, when the primer-probe combination is used to detect the methylation level at specific sites of the TRIB3 gene, it can be used for the auxiliary diagnosis, early screening, or risk assessment of colorectal cancer; when the primer-probe combination is used to detect the methylation level at specific sites of the MAPT gene, it can be used for the auxiliary diagnosis, early screening, or risk assessment of gastric cancer.

This invention relates to a method for constructing a prognostic assessment model for acute myeloid leukemia (AML) with NPM1 mutations. The method includes the following steps: (1) collecting and detecting gene mutation data required for the prognostic assessment system of AML patients with NPM1 mutations, including the mutation status of FLT3-ITD, IDH1, and SRSF2 genes; (2) assigning values ​​according to the mutation status of FLT3-ITD, IDH1, and SRSF2; (3) substituting the values ​​assigned in step (2) into the formula: mNPM1 AML risk score = 1.077 × FLT3-ITD - 0.800 × IDH1 + 1.512 × SRSF2, and performing grouped and parallel prognostic assessments on the calculated values. This invention provides a prognostic assessment model for AML with NPM1 mutations, providing important guidance for the treatment of such patients and having good clinical application value.

Provided in the present invention is an antibody against human CD3 or an antigen-binding fragment thereof; also provided are a nucleic acid molecule encoding the antibody, an expression vector expressing the antibody and a host cell, as well as a method for producing the antibody. In addition, also provided in the present invention are a pharmaceutical composition comprising the antibody or the antigen-binding fragment thereof, a use thereof in the preparation of a drug, and a use of the drug as a drug for the prevention and / or treatment of various diseases, including tumors, organ transplantation and autoimmune diseases.

The application selects highly conservative and excellent interspecies specificity UL49A gene as a target from multiple strains of HSV-1 and related herpes simplex virus (HSV-2, EBV, CMVh and VZV) coding sequences through bioinformatics whole genome alignment, designs and selects specific primer probe combination 124F / 124R / 124P, and establishes a HSV-1 probe method fluorescence quantitative PCR detection system targeting UL49A gene. Based on the primer probe group, the HSV-1 detection kit can be prepared, which is suitable for clinical biological samples such as lesion secretions, aqueous humor and blood, especially can provide a rapid, accurate and high-sensitivity etiological diagnosis scheme for HSV-1 related keratitis, and has good clinical application value.

The application discloses a kind of risk factors prediction model of occurrence of initial treatment bacterium positive pulmonary tuberculosis lung cavity, belong to medical data processing technical field, the clinical data of initial treatment bacterium positive pulmonary tuberculosis patient is collected in the application, using single factor analysis, Lasso regression analysis and multivariate Logistic regression analysis, male, diabetes, smoking, white blood cell count and platelet / hemoglobin ratio are screened as the independent risk factors of initial treatment bacterium positive pulmonary tuberculosis causing lung cavity, and based on this, nomogram prediction model is constructed.H-L goodness-of-fit test, receiver operating characteristic curve and decision curve analysis are verified, the model has higher prediction efficiency and clinical practical value.The application can help clinical advance to predict and intervene the cavity caused by high-risk population of pulmonary tuberculosis, provide new ideas for the diagnosis and treatment of bacterium positive pulmonary tuberculosis causing cavity.

The invention relates to the technical field of traditional Chinese medicine, and particularly discloses a traditional Chinese medicine composition for treating different syndrome types of stomach diseases and a preparation method thereof.The traditional Chinese medicine composition is prepared from, by weight, 15-25 parts of fried szechwan chinaberry fruit, 8-12 parts of radix bupleuri and 15-35 parts of fried fructus perillae. The traditional Chinese medicine composition is prepared from 40-60 parts of trogopterus dung, 15-35 parts of herba patriniae, 8-12 parts of radix scutellariae, 15-25 parts of radix codonopsis, 30-50 parts of fructus jujubae, 15-25 parts of radix paeoniae alba, 15-25 parts of pericarpium citri reticulatae, 2-6 parts of radix et rhizoma rhei, 8-12 parts of radix glycyrrhizae preparata and 10-35 parts of herba agrimoniae. The traditional Chinese medicine further comprises 15-35 parts of cuttlebone, 3-5 parts of calcined concha arcae, 40-60 parts of trogopterus dung, 8-12 parts of roasted rhizoma atractylodis macrocephalae, 15-25 parts of poria cocos, 4-8 parts of Chinese prickly ash, 8-12 parts of fructus amomi, 8-12 parts of fructus amomi rotundus and 5-10 parts of fructus evodiae. A complementary treatment system is formed for the four clinical common syndromes of spleen and stomach qi deficiency and water dampness, cold-dampness and qi stagnation and cold-heat intermingling, liver-stomach disharmony, cold-heat intermingling type reflux disease and phlegm-blood stasis type gastric polyp respectively and accurately.

This application relates to the field of intelligent healthcare, specifically to a method, device, and program product for predicting the risk of bladder cancer muscle invasion based on three-dimensional reconstruction. The method includes: S1, acquiring tumor imaging data from a cancer patient; S2, performing three-dimensional reconstruction using the tumor imaging data to obtain a three-dimensional tumor model; S3, identifying the absolute maximum distance between any two points on the three-dimensional tumor model in a three-dimensional coordinate system as the major axis; generating a major axis plane from the major axis, and calculating the maximum minor axis perpendicular to the major axis plane to obtain the minor axis; S4, calculating the ratio of the major axis to the minor axis to obtain the tumor's major-minor axis ratio; S5, predicting the risk of muscle invasion using the tumor's major-minor axis ratio to obtain the prediction result. This application predicts intermuscular invasion from multiple dimensions and has significant clinical value.

This invention provides the application of costunolide in the preparation of a drug for preventing posterior capsule opacification. Costunolide effectively reduces posterior capsule opacification after extracapsular lens extraction (ECLE) and exhibits significant anti-inflammatory and anti-fibrotic effects in both in vitro and in vivo models. Mechanistic studies have identified matrix metalloproteinase 8 (MMP8) as a key downstream effector of costunolide, confirming that costunolide inhibits MMP8 expression, thereby blocking the transformation of lens epithelial cells (LECs) into mesenchymal cells and subsequent fibrotic processes. This lays a solid foundation for developing targeted therapies for posterior cataract, a common surgical complication.

The application discloses a hemorrhoid operation system, which comprises a safe blocking component, an intracavitary radial feed drainage knife group, and an intracavitary negative pressure forced stasis discharge component. The safe blocking component is used for sealing with the intestinal wall to block the downward flow of intestinal excrement. The intracavitary radial feed drainage knife group is used for puncturing a lesion. The intracavitary negative pressure forced stasis discharge component is used for negative pressure action on the punctured lesion to discharge stasis. Thus, the downward flow of intestinal excrement can be blocked to avoid infection of a surgical wound. The cyst at the hemorrhoid lesion is punctured to dredge the stasis discharge channel, and the intracavitary negative pressure forced stasis discharge component is used to forcibly discharge the stasis in the cyst. Compared with traditional hemorrhoid medical instrument operation, the hemorrhoid operation system does not need to cut off the epidermis of the internal hemorrhoid lesion and the large intestinal muscle, has no contraindicated population, and will not cause serious complications such as rectal stenosis and death, and has strong safety and significant clinical application value.

The utility model discloses a kind of embedding boxes for congenital megacolon specimen, it is related to the field of tissue embedding technology.It includes box body with filter hole, circular ring, tight knob column, helical segmentation strip and box cover.Circular ring is fixed along inside of side plate, helical segmentation strip is coiled in spiral, one end is connected with the tight knob column of center, the other end is fixed in circular ring, can adjust interval with knob column rotation to hold or release specimen.Tight knob column contains shaft sleeve, shaft cap and split type knob spoon, through hexagonal structure cooperation and the bistable clamping of arc spring and clamping groove, the clutch and stable rotation of knob spoon and shaft cap are realized, control helical strip tightness.Box body, circular ring, helical strip etc.are provided with round hole to ensure reagent penetration, box cover buckle sealing, label area is provided in front.The design realizes specimen integration fixation, maintains morphological continuity, simplifies material taking, embedding process, improves pathological diagnosis efficiency and accuracy.

The application relates to the cross field of artificial intelligence and environmental health, and discloses a membrane nephropathy prognosis risk stratification method based on exposure spectrum and clinical indicators. First, demographic information, clinical detection indicators and various environmental pollutant exposure data of patients are collected to construct a multi-level exposure spectrum feature, including single pollutant concentration, same type comprehensive exposure factor and pollutant synergistic interaction term. Then, the exposure spectrum and the clinical indicators are fused, various machine learning algorithms are used to construct candidate prediction models, the optimal prognosis risk stratification model is screened through cross validation and multiple performance indicators, and the SHAP value is used to quantify the contribution degree of each feature. Further, a double machine learning method is used to control confounding variables and estimate the causal effect of key pollutants on prognosis. On this basis, individual risk stratification and main risk driving factor identification of membrane nephropathy patients are realized, and scientific basis and guidance are provided for clinical decision making.

This invention discloses a positioning pad for maintaining body position after total hip arthroplasty. The positioning pad includes a first support pad and a second support pad, which are fixedly connected at a 90° angle or rotatably connected via a rotating mechanism. The end of the second support pad away from the first support pad has a lifting adjustment mechanism configured to adjust the angle between the first support pad and the horizontal direction by adjusting the height of the second support pad. The first support pad has a pair of anti-rotation pillows extending along a 30° hip abduction direction. The second support pad, away from the first support pad, has a T-shaped shoe extending along a 30° hip abduction direction. This positioning pad, by providing appropriate support and comfort, ensures that the patient maintains this specific position during recovery, which not only helps reduce sciatic nerve tension and displacement, lowering the incidence of postoperative complications, but also helps promote the patient's rehabilitation process.

The invention discloses a schistosoma japonicum katsurada source exosome miRNA fluorescent quantitative PCR (polymerase chain reaction) detection kit and a use method thereof. The kit comprises a stem-loop method reverse transcription primer used for reverse transcription of the sja-miR-61, a qPCR (quantitative polymerase chain reaction) upstream primer used for amplification of the sja-miR-61, a qPCR downstream primer used for amplification of the sja-miR-61, a stem-loop method reverse transcription primer used for reverse transcription of the sja-miR-7-5p, a qPCR upstream primer used for amplification of the sja-miR-7-5p and a qPCR downstream primer used for amplification of the sja-miR-7-5p. The upstream primer is used for amplifying a reference gene U6, and the downstream primer is used for amplifying the reference gene U6. The fluorescent quantitative PCR method is used for detecting schistosoma japonicum source exosome miRNA, the detection sensitivity is high, the specificity is good, the operation is simple, the trauma is small, and the method is suitable for popularization and application.

The application provides a composite preservative for fecal microorganisms and metabolites thereof and a same-tube multi-omics joint detection integrated technology. The composite preservative contains main components of cetyltrimethylammonium bromide (CTAB), ammonium chloride (NH4Cl) and phosphate buffered saline (PBS), can keep the stability of microorganisms and metabolites thereof in a fecal sample during storage and transportation at room temperature, and guarantees the extraction efficiency of deoxyribonucleic acid (DNA) of the microorganisms in the fecal sample. The application also eliminates the influence of the preservative components on metabolite detection by adding a treatment reagent, significantly improves the response and ionization efficiency of the fecal sample metabolites in mass spectrometry, and thus improves the sensitivity and accuracy of the detection results. The application not only solves the timeliness problem of fecal sampling, but also provides a technical basis and idea for the feasibility study of the same-tube multi-omics of the fecal sample, and has a broad research and clinical application prospect.

The invention relates to the technical field of traditional Chinese medicines, and particularly discloses a traditional Chinese medicine for treating intractable insomnia and a preparation method thereof. The traditional Chinese medicine is prepared from the following raw materials in parts by weight: 18 to 22 parts of radix salviae miltiorrhizae, 12 to 18 parts of radix curcumae, 12 to 18 parts of rhizoma chuanxiong, 8 to 12 parts of radix angelicae sinensis, 6 to 12 parts of radix polygoni multiflori preparata, 14 to 16 parts of semen persicae, 10 to 14 parts of radix angelicae, 15 to 21 parts of cortex albiziae, 10 to 14 parts of poria cocos, 13 to 17 parts of pericarpium citri reticulatae, 8 to 10 parts of ramulus cinnamomi, 8 to 14 parts of caulis bambusae in taeniam, 3 to 5 parts of cortex cinnamomi and 20 to 25 parts of semen boitae. Aiming at the core pathogenesis of blood stasis and qi depression, malnutrition, internal resistance of phlegm and turbidity and insufficiency of yang qi, salvia miltiorrhiza and platycladi seeds are used as monarch drugs, radix curcumae and ligusticum wallichii are used as ministerial drugs, and angelica sinensis, radix polygoni multiflori preparata, peach kernels, radix angelicae, cortex albiziae, poria cocos, pericarpium citri reticulatae and caulis bambusae in taeniam are used as adjuvant drugs; cassia twig and cinnamon are used as conductant drugs, so that the traditional Chinese medicine composition has an exact treatment effect on intractable insomnia, is high in safety and has an important clinical application value.

The invention relates to the technical field of antibodies, in particular to an abnormal prothrombin (PIVKA-II) antibody or a conjugate thereof and application thereof. The antibody or the conjugate thereof comprises a heavy chain variable region VH and a light chain variable region VL, the amino acid sequences of HCDR1, HCDR2 and HCDR3 of the heavy chain variable region VH are respectively shown as SEQ ID No: 1-3, and the amino acid sequences of LCDR1, LCDR2 and LCDR3 of the light chain variable region VL are respectively shown as SEQ ID No: 4-5; the antibody conjugate is formed by coupling the antibody with a marker or a solid-phase carrier. The antibody disclosed by the invention has high binding activity and affinity to PIVKA-II, and the specificity, sensitivity, reliability and accuracy detection of trace PIVKA-II in a sample can be realized in a double-antibody sandwich manner.

The application discloses a novel morphological staining solution and a human microecological detection method based on artificial intelligence, and relates to the technical field of medical detection. The novel morphological staining solution comprises a composite staining solution A and a composite staining solution B, the composite staining solution A comprises the following components: 0.05-1.5 wt% thiazine dye, 0.05-1.5 wt% composite stabilizer, 0.051-1.05 wt% permeability regulator and 0.001-0.05 wt% multifunctional blueing agent; and the composite staining solution B comprises the following components: 0.01-0.5 wt% eosin, 0.001-1 wt% methylene blue and 0.001-0.05 wt% permeability regulator. The novel morphological staining solution is composed of the composite staining solution A and the composite staining solution B, can present different markers under visible light and fluorescent dark field at the same time, and can be quantitatively measured by a computer; in addition, rare earth is used to enhance blueing, Eu 3+ is added in the blueing solution to coordinate with the DNA phosphate groups of cell nuclei, so that nuclear staining produces red fluorescence under 360 nm ultraviolet excitation, and the nuclear-cytoplasm contrast under a low-power lens can be improved, thereby providing specific staining effects for subsequent computer recognition.

The application discloses a preoperative three-dimensional reconstruction assisted surgery planning system, and belongs to the technical field of medical image processing and surgery planning. The system comprises an image data acquisition module, a three-dimensional reconstruction module, a structure identification module, a topological relation construction module, a risk assessment module, a path generation module, a path optimization module and a result output module. The image data acquisition module is used for acquiring medical image data of a region to be operated of a patient. The three-dimensional reconstruction module is used for constructing a three-dimensional anatomical model. The structure identification module is used for identifying target organ structures and key anatomical structures. The topological relation construction module is used for establishing spatial relations between anatomical structures. The risk assessment module is used for identifying risk regions in the operation region and generating a risk distribution model. The path generation module is used for generating candidate operation paths. The path optimization module is used for determining an optimal operation path. The result output module is used for outputting the three-dimensional anatomical model and operation planning results.

The application discloses an incomplete multi-omics cancer subtype identification method, system, device and medium, and belongs to the technical field of bioinformatics and artificial intelligence. The method comprises the following steps: incomplete multi-omics data acquisition and preprocessing; constructing an entry-level observation mask matrix and a view availability mask matrix; constructing a feature module in the omics based on a granulocyte division; extracting a module-level skeleton representation; recovering an entry-level missing value based on the skeleton structure; constructing a multi-expert skeleton recovery integrated result; constructing a central feature matrix of the feature module; constructing a cross-omics consensus structure space; performing a mask-aware skeleton consensus alignment; performing adaptive view weighting based on structure reliability; performing sample-level mask-aware fusion; and outputting a cancer subtype clustering result. The application provides reliable technical support for cancer typing research, patient stratification analysis, prognosis evaluation and precision medicine auxiliary decision-making.

The application provides a gadolinium-based composite material with microwave response heat production and double-mode imaging effect, and preparation and application thereof. The gadolinium-based composite material prepared by the application is applied to the fields of microwave hyperthermia and double-mode imaging of tumors, and has good clinical application value. The gadolinium-based composite material synthesized by the application has low cytotoxicity and good cell internalization efficiency, can significantly increase the killing effect of microwaves on tumor sites, and has higher cell internalization efficiency and better microwave heating performance through adjustment of the morphology of the gadolinium-based composite material. In addition, Gd 3+ in the nanomaterial has a nuclear magnetic resonance enhancement effect, and the loaded fluorescent dye has a fluorescence imaging effect, so that the material has double-mode imaging function and can be used as a microwave diagnosis and treatment agent to realize tumor diagnosis and treatment integration.

The application discloses application of a protein degradation targeting compound to preparation of an antitumor drug. The compound has excellent antitumor cell proliferation activity, and test example data show that multiple compounds have significant concentration-dependent proliferation inhibition effect on colorectal cancer cells. Moreover, the compound has the potential to achieve sustained drug efficacy at a low dose. The compound has good antiproliferation activity on colorectal cancer cells, and therefore has definite potential for preparation of an anticancer drug.

This invention relates to the field of medical and nursing device technology, and discloses a hammock-type assisted turning bed and a turning method, including a bed frame body. The bed frame body includes multiple functional segments arranged sequentially along its length. Each functional segment is equipped with a bed surface driving mechanism and a support adjustment mechanism. The bed surface driving mechanism includes a roller winding device and a bed surface. Two roller winding devices are symmetrically arranged on both sides of the bed frame body of the functional segment. The two ends of the bed surface are wound around the rollers of the corresponding roller winding devices. The support adjustment mechanism is located below the bed surface and includes multiple electric cylinders arranged along the width direction of the bed frame body. The hammock-type assisted turning bed and turning method provided by this invention solve the problems of high risk of shear friction injury, high risk of pressure sores, poor comfort, and insufficient safety and adaptability in the practical application of existing technologies.

This invention relates to the field of clinical virus detection, and more particularly to a protein dot blot detection kit, detection method, and application for varicella-zoster virus (VZV). The kit includes: a solid-phase carrier membrane for binding and immobilizing proteins in the sample; a blocking agent for blocking non-specific binding sites on the solid-phase carrier membrane; a first antibody that specifically recognizes the VZV antigen; an enzyme-labeled second antibody that binds to the first antibody; and a chromogenic solution for generating a detection signal. This kit is based on protein dot blot technology, detecting proteins with high specificity and a low false-positive rate. The experimental cycle is short, taking only 2-3 hours from sample pretreatment to signal detection, much faster than virus culture (requiring 4-7 days) and PCR detection (although fast, prone to false positives or false negatives), providing rapid evidence for clinical treatment, and is particularly suitable for the diagnosis of acute herpes zoster.