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MiRNA (micro Ribonucleic Acid) marker and kit related to postmenopausal osteoporosis

A technology of osteoporosis and markers, applied in the field of miRNA markers and kits, can solve the problems of slow osteoporosis and difficult early diagnosis of clinical patients

Active Publication Date: 2017-12-01
WUHAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the occurrence of osteoporosis is very slow, and the change is not obvious in a short period of time. It requires long-term observation and continuous detection. Once bone loss is found, the decrease in bone density is difficult to reverse, which brings great benefits to the early diagnosis of clinical patients. come with great difficulty

Method used

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  • MiRNA (micro Ribonucleic Acid) marker and kit related to postmenopausal osteoporosis
  • MiRNA (micro Ribonucleic Acid) marker and kit related to postmenopausal osteoporosis
  • MiRNA (micro Ribonucleic Acid) marker and kit related to postmenopausal osteoporosis

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Embodiment 1

[0048] Experimental process: Collect blood samples from 15 postmenopausal osteoporosis patients and 15 healthy samples, collect serum after centrifugation, and use a kit to extract total RNA (including miRNA) in serum, perform RT-PCR after reversal, and perform RT-PCR according to the CT value Perform statistical analysis.

[0049] The expression levels of miR-338-3p and miR-3065-5p in the blood of postmenopausal osteoporosis patients were detected, as shown in Figure 2A As shown in and 2B, the results show that the expression levels of miR-338-3p and miR-3065-5p are significantly higher than that of the control healthy group samples, wherein each black dot represents each sample, and the higher the position of the point, the higher the content. many. at the same time as Figure 2C As shown, the results of ROC analysis showed that miR-338-3p and miR-3065-5p had high diagnostic value.

[0050] Establish a mouse model of osteoporosis with bilateral ovariectomy:

[0051] The...

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Abstract

The invention provides a miRNA (micro Ribonucleic Acid) marker and a kit related to postmenopausal osteoporosis and particularly relates to application of a miR-338 cluster as a postmenopausal osteoporosis marker. According to the invention, high expression of the miR-338 cluster such as miR-338-3p and / or miR-3065-5p in postmenopausal osteoporosis patients is found; the miR-338 cluster can be used as a marker for the postmenopausal osteoporosis and is used for preparing an auxiliary diagnosis kit for the postmenopausal osteoporosis; the auxiliary diagnosis kit can be used for specifically detecting the expression quantity of the miR-338 cluster in tissues and is early and effectively used for early auxiliary diagnosis of clinical postmenopausal osteoporosis without noninvasive. Besides, an inhibitor of the miR-338 cluster has the efficacy of promoting osteogenesis, so that the postmenopausal osteoporosis is effectively treated or prevented.

Description

technical field [0001] The present invention relates to the field of genetic engineering, and more specifically relates to miRNA markers and kits related to postmenopausal osteoporosis. Background technique [0002] microRNAs (miRNAs) represent a specific small molecule of non-coding RNA that regulates gene expression post-transcriptionally. A single miRNA can interact with transcription factors by repressing the expression of a series of related functional genes. So far, many studies have proved that blocking miRNA can block the process of osteoporosis. Multiple independent animal model experiments have also confirmed that many miRNAs are altered in the expression of osteoporosis and are also involved in the pathological process of osteoporosis. Since miRNA was first discovered in 1993, more and more scientific researchers have paid more and more attention to miRNA. The role of miRNA in the occurrence and development of various diseases has also been extensively and deep...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68A61K45/00A61P19/10
CPCA61K45/00C12Q1/6883C12Q2600/178C12Q2600/158C12N15/11C12N15/63
Inventor 刘欢林楚娇陈智
Owner WUHAN UNIV
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