Novel vascularized tissue-engineered bone and construction method thereof

A tissue-engineered bone and vascularization technology, used in bone implants, medical science, prostheses, etc., can solve the problem that tissue-engineered bone has an unsatisfactory osteogenesis promotion effect, limited blood supply in the central part of the stent, and vascular pedicle length requirements. It can achieve the effect of increasing the blood supply and nutrition area, safe and effective vascularization, and clinical feasibility.

Inactive Publication Date: 2014-05-07
PLASTIC SURGERY HOSPITAL CHINESE ACAD OF MEDICAL SCI
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  • Summary
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Problems solved by technology

The vascular bundle presetting technology is based on the principle of axial blood supply in the marrow cavity of bone tissue. The distally ligated vascular bundle passes through the hole or groove in the central part of the tissue engineered bone. The vascular ingrowth and nutrition are from the center to the periphery. Lei Cai inoculated HA scaffolds with BMSCs from Beagle dogs, and repaired fibula bone defects in Beagle dogs with the help of vascularization with pre-prepared vascular bundles. Wang inoculated BMSCs with TCP scaffolds and pre-prepared saphenous arteriovenous bundles in rabbits to construct vascularized tissue engineering. bone and repair rabbit femoral bone defect, but because the above traditional vascular bundle prefabrication techniques all use the arteriovenous bundle to be placed in a separate hole or groove of a large-scale tissue engineering bone scaffold for prevascularization, the vascularized area is limited, The speed of vascularization is slow, and the promotion effect on tissue engineering bone osteogenesis is not ideal; the arter

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  • Novel vascularized tissue-engineered bone and construction method thereof
  • Novel vascularized tissue-engineered bone and construction method thereof

Examples

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Effect test

Embodiment 1

[0021] Example 1: Method for vascularization of pre-placed vascular loops in tissue engineered bone

[0022] See figure 1 , take a commercial β-TCP stent, prepare it into a cylindrical shape with a bottom diameter of 12 mm and a height of 10 mm, carefully punch out two axial hollow channels with a diameter of 3 mm inside the stent, and round the exit holes at both ends. After disinfection treatment, BMSCs were inoculated and cultured in vitro for 2 weeks. The saphenous artery and vein were separated about 4cm, and the saphenous artery and vein were ligated at the distal end. The BMSCs / β-TCP complex was replaced with serum-free medium 12 hours before implantation to reduce the impact of residual fetal bovine protein as an antigen on the recipient after implantation. The BMSCs / β-TCP cell scaffold complex was placed on the original saphenous arteriovenous vascular bed, and two sutures were reserved to pass through the tunnel of the cell scaffold complex to facilitate postoperat...

Embodiment 2

[0024] Example 2: Method for vascularization of pre-placed vascular bundles in tissue engineered bone

[0025] See figure 2, take a commercial β-TCP scaffold, prepare it into a cylindrical shape with a bottom diameter of 12mm and a height of 10mm, and carefully punch out two axial hollow channels with a diameter of 3mm inside the scaffold. After aseptic treatment, BMSCs are inoculated and cultured in vitro to induce 2 weeks. The saphenous artery and vein were separated about 4cm, and the saphenous artery and vein were ligated at the distal end. The BMSCs / β-TCP complex was replaced with serum-free medium 12 hours before implantation to reduce the impact of residual fetal bovine protein as an antigen on the recipient after implantation. The BMSCs / β-TCP cell scaffold complex was placed on the original saphenous arteriovenous vascular bed, and two sutures were reserved to pass through the tunnel of the cell scaffold complex to facilitate postoperative fixation. Using the uncut...

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Abstract

The invention relates to a novel vascularized tissue-engineered bone which comprises a cylinder support material, two internal axially-preset circular tunnels and arteriovenous blood vessel peduncles. The cylinder support material is a medical absorbable material, the two internal axially-preset circular tunnels are parallel to each other, an outlet circular chamfer is arranged at an outlet, and human or animal osteogenic induction stem cells are evenly distributed inside the circular tunnels. The arteriovenous blood vessel peduncles comprise arteries, veins and arteriovenous grafts, the arteries, the veins and the arteriovenous grafts are arranged on receptor blood vessel peduncles, the arteriovenous blood vessel peduncles are arranged in the support material and are arranged in parallel in a separated mode, and distal blood vessels are anastomosed or ligatured. According to the novel vascularized tissue-engineered bone, the tissue-engineered bone can be vascularized fast, fully, safely and effectively after transplantation, the clinical effects are effectively improved, good nutrition supply and the good metabolism environment are provided for transplanted cells, the osteogenic activity of the tissue-engineered bone after transplantation is promoted, and the wide clinical application of the grafts of the tissue-engineered bone is promoted.

Description

technical field [0001] The invention relates to the field of tissue engineering, in particular to a novel vascularized tissue engineering bone and a construction method thereof. Background technique [0002] The concept of tissue engineering (Tissue Engineering) was first proposed by Langer and Vacanti. According to the principles of cell biology and engineering, tissue cells with specific biological activities are combined with biomaterials to construct tissues and organs in vitro or in vivo. The science of maintaining, repairing, regenerating or improving the function of damaged tissues and organs. In the field of bone tissue engineering, the treatment of large bone defects with tissue engineered bone gradually shows attractive prospects, but it also faces some new problems. [0003] The vast majority of tissue cells in the living body are supplied with oxygen and nutrients by blood, limited by the diffusion of oxygen in the tissue, the oxygen supply range of capillaries ...

Claims

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Application Information

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IPC IPC(8): A61F2/28
CPCA61F2/28A61F2002/30001
Inventor 曹谊林肖苒王黔吴骁伟
Owner PLASTIC SURGERY HOSPITAL CHINESE ACAD OF MEDICAL SCI
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