Tissue engineered bone cartilage frame and preparation method thereof

Abstract

The invention belongs to the field of cartilage tissue repair and tissue engineering and provides a tissue engineered bone cartilage frame and a preparation method thereof. Particularly, marrow removal and degreasing are carried out on allogeneic or xenogeneic cancellous bones to obtain cancellous bone columns with experimentally or clinically required diameters, and then a tissue engineered bonecartilage frame with a completely decalcified upper layer and a superficially decalcified lower layer is obtained via layered decalcification. The upper layer of the frame is used as the framework part of the tissue engineered cartilage frame or an engineering cartilage frame, and the lower layer thereof can be used as a bone forming frame, an engineering bone bracket and the fixed pivot of the engineered cartilage, and can be used for constructing an integrative tissue engineered bone cartilage or directly transplanted for the cartilage or bone cartilage repair. Compared with the existing tissue engineered bone cartilage frame, the invention has the advantages of integration (no interface mechanical defect), and good compatibility of a pore structure and the tissue, has better compression resisting, tensile resisting and shear resisting properties, and is convenient for the minimally invasive surgery operation under an arthroscope.

Description

technical field [0001] The invention belongs to the field of cartilage tissue repair and tissue engineering. The invention provides a method for preparing tissue engineered osteochondral scaffold and the tissue engineered osteochondral scaffold prepared by the method. Specifically, the present invention uses cancellous bone as a raw material and utilizes layered decalcification technology to prepare tissue engineered osteochondral scaffolds, and the prepared tissue engineered osteochondral scaffolds can be used to construct osteochondral complexes. Background technique [0002] Injuries and lesions of articular cartilage are common diseases, and it is difficult to repair by itself. Tissue-engineered cartilage is the most promising repair method. However, the following problems need to be solved urgently in the research of tissue engineering of articular cartilage: (1) When repairing articular cartilage, it is often necessary to repair subchondral bone. Clinically, simple ...

AI Technical Summary

Problems solved by technology

(2) The fixation problem of tissue engineered articular cartilage products has not been effectively solved

(3) Existing tissue engineered cartilage has biomechanical defects

(4) Poor integration between tissue engineered cartilage and subchondral bone

[0005] The composite osteochondral scaffold using the natural gel-like material as the upper cartilage scaffold has the following problems: ①The upper layer material has poor mechanical properties; ②There are mechanical defects between the interfaces, which are easy to tear off and have poor shear resistance; ③Gel-like scaffolds After implanting in the body, it absorbs water and deforms, turning into a gel again, and the mechanical properties of the cartilage layer are further weakened; ④The operation is relatively complicated; ⑤The lower layer of hydroxyapatite degrades slowly, which affects the healing with the surrounding bone

[0010] There are many studies and patents on the application of decalcified bone matrix as a scaffold for cartilage tissue engineering, but they are all only used for cartilage tissue engineering, and the aforementioned fixation problems still exist when implanted in the body

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