6 results about "Bones cartilage" patented technology

The invention relates to the technical field of biomedical materials, in particular to multi-material 3D printing bionic gradient soft-hard integrated hydrogel as well as a preparation method and application thereof. Based on a copolymerization system of high-strength supramolecular N-acryloylglycinamide and vinyl phosphoric acid, an NAGA aqueous solution and an NAGA-VPA aqueous solution are printed into hydrogel of a gradient structure by adopting a DLP multi-material printing technology, then the hydrogel is sequentially soaked in a calcium-phosphorus mixed aqueous solution (Ca / P = 1.67), ammonia water, a calcium chloride aqueous solution and deionized water, and the hydrogel is prepared through a hydrothermal method. Therefore, in-situ gradient deposition and curing of hydroxyapatite (HAP) are realized. The multi-material 3D printing bionic gradient soft-hard integrated hydrogel prepared by the method has a continuous biochemical-mechanical gradient, a surface layer low-modulus area is matched with cartilage performance, a deep layer high-mineralization area is integrated with bone tissue, the HAP crystallinity of a transition area is in spatial dependence distribution, and the hydrogel is suitable for osteochondral defect repair and manufacturing of bionic joint prostheses.

The invention relates to the technical field of biomedical materials, and provides a preparation method of a fibroin nanofiber reinforced silk protein gel material, the gel material and application, fibroin nanofibrils are prepared through a mechanical grinding method and introduced into composite hydrogel, the Young modulus reaches 4.68 MPa, and the fibroin nanofiber reinforced silk protein gel material is suitable for osteochondral repair and drug delivery.

The application discloses an enzyme response self-assembly polypeptide modified methacrylating polymer and a preparation method and application thereof, and the enzyme response self-assembly polypeptide modified methacrylating polymer has a structure of formula 1. The application converts an endogenous tissue-specific marker alkaline phosphatase concentration gradient into a dynamic gradient evolution of the mechanical properties of a scaffold. By introducing an enzyme response self-assembly polypeptide into a methacrylating polymer structure, the scaffold is subjected to molecular level dynamic self-assembly under the catalysis of phosphatase, and a continuous mechanical gradient is formed from a cartilage region to a subchondral bone region. The process does not require external intervention and is completely regulated by a local microenvironment, thereby real-time adapting to the dynamic requirements of 'early proliferation-late differentiation' of bone cartilage repair. Compared with the prior art, the application not only breaks through the mismatch between a static gradient and a dynamic microenvironment, but also precisely regulates mechanical signals at a molecular scale, and significantly improves the spatial specificity and time synchronism of stem cell differentiation.

The application provides a kind of osteochondral repair material and its preparation method and application, belong to the technical field of biomedical materials.The application is prepared hyaluronic acid-collagen with collagen as organic matrix, through the crosslinking reaction mediated by crosslinking agent;Hydroxyapatite is deposited in situ in the self-assembly process of collagen fiber through in vitro biomimetic mineralization technology, and mineralized collagen with different mineralization degrees is prepared;Iterative layered freeze-drying technology is used to construct biomimetic cartilage layer, calcification layer and subchondral layer in turn, and transforming growth factor-beta 3 and platelet-derived growth factor-BB are also loaded by physical adsorption to synergistically promote the repair and regeneration of osteochondral defects.The preparation process of the application is mild and controllable, and the prepared osteochondral repair material has good biocompatibility and intelligent active factor delivery function, and has important application prospect in the field of osteochondral tissue engineering and clinical defect repair.

The invention belongs to the technical field of biomedical materials, and particularly relates to a highly-bionic gradient artificial cartilage scaffold material, a preparation method and application, the highly-bionic gradient artificial cartilage scaffold material comprises a hyaline cartilage layer, a calcified cartilage layer and a subchondral bone layer, the calcified cartilage layer is composed of mineralized I-type collagen fibers containing 30% of nano-hydroxyapatite, and the subchondral bone layer is composed of mineralized I-type collagen fibers containing 60% of nano-hydroxyapatite; the prepared scaffold highly simulates natural osteochondral tissues, has similar composition, nano structure and mechanical properties with the natural osteochondral tissues, and presents an obvious gradient; the hydrogel has excellent biocompatibility and biological activity, and cell proliferation and gradient differentiation of bone marrow mesenchymal stem cells can be remarkably promoted; in a rabbit osteochondral defect model, osteochondral tissue regeneration is remarkably promoted, and the compound can be applied to clinical treatment of osteoarthritis.

The application relates to the field of biomedical materials, and particularly discloses a collagen-calcium-phosphorus-based flexible ceramic bone cartilage repair body and a preparation method thereof. The collagen-calcium-phosphorus-based flexible ceramic bone cartilage repair body comprises a calcium-phosphorus-based flexible ceramic bone phase layer, wherein beta-tricalcium phosphate ceramic microspheres and calcium phosphate powder are used as solid phase components, and distilled water or pure water, sodium hydroxide and disodium hydrogen pyrophosphate are used as liquid phase components; at least one anchoring harbor is arranged on the top surface of the bone phase layer; and a collagen porous cartilage layer is prepared by using a collagen solution and a photocuring system to prepare printing ink and then performing 3D printing. The collagen-calcium-phosphorus-based flexible ceramic bone cartilage repair body and the preparation method thereof are substantially improved in structural design, interface combination, mechanical properties, clinical operability and repair effect, and embody the integrated innovation of "flexible ceramic-collagen-anchoring structure-3D printing".