21 results about "Bones cartilage" patented technology

The invention discloses a biphasic hydrogel scaffold of a bionic bone cartilage matrix as well as a preparation method and application of the biphasic hydrogel scaffold, and belongs to the technical field of tissue engineering. The biphasic hydrogel scaffold of the bionic bone cartilage matrix provided by the invention comprises a hard bone matrix phase and a cartilage matrix phase. Wherein hard chitosan hydrogel with a porous structure is used as a hard bone matrix phase, and soft chitosan hydrogel is used as a cartilage matrix phase. The biphase hydrogel with the asymmetric matrix stiffness and the biochemical microenvironment can effectively release active substances in a controlled mode, the release period exceeds 21 days, the time requirement for osteochondral repair is met, and lasting support is provided for proliferation and differentiation of cells. Meanwhile, the biphase hydrogel provided by the invention has good biological safety, two matrix phases have different mechanical properties, the biphase hydrogel adapts to in-vivo complex physiological and mechanical environments, the fate of stem cells can be directionally regulated and controlled, synchronous regeneration and repair of osteochondral tissues are realized, and the biphase hydrogel has a wide application prospect.

The invention relates to the technical field of biomedical materials, in particular to multi-material 3D printing bionic gradient soft-hard integrated hydrogel as well as a preparation method and application thereof. Based on a copolymerization system of high-strength supramolecular N-acryloylglycinamide and vinyl phosphoric acid, an NAGA aqueous solution and an NAGA-VPA aqueous solution are printed into hydrogel of a gradient structure by adopting a DLP multi-material printing technology, then the hydrogel is sequentially soaked in a calcium-phosphorus mixed aqueous solution (Ca / P = 1.67), ammonia water, a calcium chloride aqueous solution and deionized water, and the hydrogel is prepared through a hydrothermal method. Therefore, in-situ gradient deposition and curing of hydroxyapatite (HAP) are realized. The multi-material 3D printing bionic gradient soft-hard integrated hydrogel prepared by the method has a continuous biochemical-mechanical gradient, a surface layer low-modulus area is matched with cartilage performance, a deep layer high-mineralization area is integrated with bone tissue, the HAP crystallinity of a transition area is in spatial dependence distribution, and the hydrogel is suitable for osteochondral defect repair and manufacturing of bionic joint prostheses.

The invention relates to the technical field of biomedical materials, and provides a preparation method of a fibroin nanofiber reinforced silk protein gel material, the gel material and application, fibroin nanofibrils are prepared through a mechanical grinding method and introduced into composite hydrogel, the Young modulus reaches 4.68 MPa, and the fibroin nanofiber reinforced silk protein gel material is suitable for osteochondral repair and drug delivery.

The present invention discloses an integrated bone and cartilage scaffold with a directional pore structure and a preparation method thereof, which belongs to the fields of tissue engineering, biomedicine and biomanufacturing technology. The method simulates the natural material and structure of bone cartilage, uses the principle of directional crystallization to prepare a bone and cartilage scaffold with a directional structure, and uses a low-temperature deposition manufacturing process based on rapid prototyping technology to prepare a horizontally oriented cartilage layer scaffold and a vertically oriented subchondral bone layer scaffold. Between the cartilage layer and the subchondral bone layer, methacrylated gelatin capable of UV cross-linking is combined with other materials with chemical grafting and bonding effects as a transition layer, thereby preparing a tissue engineering bone and cartilage integrated composite scaffold. The present invention is aimed at the treatment of articular bone and cartilage defects, and uses a bioactive material to manufacture a bone and cartilage composite scaffold with a directional pore structure. It can promote the coordinated repair of bone and cartilage tissue according to the characteristics of different functional areas of bone and cartilage, thereby improving the repair effect of bone and cartilage defects.

The present invention provides an integrated design and manufacturing technology for a bone cartilage biomimetic gradient scaffold. The invention includes a functionally graded integrated bone cartilage biomimetic gradient scaffold, a transition structure for achieving graded transition between hard bone and cartilage tissue, and a biomimetic cartilage formation and post-processing method. The integrated bone cartilage biomimetic gradient scaffold is a porous interconnected ceramic structure that can be manufactured through integrated additive manufacturing. The transition structure is a special structure for firmly connecting hard bone and cartilage. The biomimetic cartilage formation and post-processing method involves performing special treatment on the 3D-printed bone scaffold to form biomimetic cartilage, thereby improving the strength of the cartilage layer and preventing cartilage damage caused by joint friction. The bone cartilage biomimetic scaffold structure proposed by the present invention has effective functional grading to mimic natural bone cartilage tissue, which is beneficial for preventing stress shielding and can be manufactured in one piece using 3D additive manufacturing technology.

The present application discloses a bone cartilage repair scaffold and a preparation method thereof, wherein the bone cartilage repair scaffold includes a subchondral bone layer, a calcified cartilage layer and a cartilage layer connected in sequence, the calcified cartilage layer includes a calcified cartilage upper layer, a calcified cartilage middle layer, a calcified cartilage lower layer, and a porous gel embedded in the calcified cartilage upper layer and the calcified cartilage middle layer. In an embodiment of the present application, the above-mentioned bone cartilage repair scaffold and a preparation method thereof are adopted, and a layered structure is adopted to simulate human physiological cartilage. Through the layering and gradient porosity design of the calcified cartilage layer, not only the flexibility of the scaffold is improved and the performance of the scaffold is improved, but also it can ensure that the scaffold can quickly recruit the patient's autologous blood after being implanted into the bone cartilage defect site, thereby guiding bone marrow mesenchymal stem cells to differentiate into chondrocytes in the cartilage layer and promote the generation of cartilage tissue.

The application discloses an enzyme response self-assembly polypeptide modified methacrylating polymer and a preparation method and application thereof, and the enzyme response self-assembly polypeptide modified methacrylating polymer has a structure of formula 1. The application converts an endogenous tissue-specific marker alkaline phosphatase concentration gradient into a dynamic gradient evolution of the mechanical properties of a scaffold. By introducing an enzyme response self-assembly polypeptide into a methacrylating polymer structure, the scaffold is subjected to molecular level dynamic self-assembly under the catalysis of phosphatase, and a continuous mechanical gradient is formed from a cartilage region to a subchondral bone region. The process does not require external intervention and is completely regulated by a local microenvironment, thereby real-time adapting to the dynamic requirements of 'early proliferation-late differentiation' of bone cartilage repair. Compared with the prior art, the application not only breaks through the mismatch between a static gradient and a dynamic microenvironment, but also precisely regulates mechanical signals at a molecular scale, and significantly improves the spatial specificity and time synchronism of stem cell differentiation.

The invention relates to an in-situ lubrication 3D printing Janus bone cartilage repair scaffold and a preparation method thereof.The scaffold is prepared through 3D printing and comprises a subchondral bone layer and a cartilage layer, the subchondral bone layer is prepared from GelMA and NAGA loaded nHA, and bone healing is promoted; the cartilage layer is provided with zwitterionic monomers, and in-situ lubrication is achieved. In-situ lubrication can be realized through lubrication modification of the cartilage layer scaffold structure, and bone healing is promoted by loading hydroxyapatite on the subchondral bone layer scaffold.

The application relates to the technical field of femoral head necrosis treatment, in particular to a bionic artificial balloon type half hip joint, which comprises a sleeve, a femoral neck, a fixing screw and a femoral neck limiting nut, the sleeve is fixed on a femur through the fixing screw, the femoral neck is slidingly connected in the sleeve, a balloon is fixed on the outer periphery of the top section of the femoral neck, the inside of the femoral neck is hollow, and a side hole is arranged at the top end of the femoral neck and communicates with the balloon, and the femoral neck limiting nut is used for being fixed in the sleeve to position the femoral neck. The balloon type femoral head is compared with the metal and ceramic femoral heads, the balloon type femoral head is more matched with the shape of the acetabulum in terms of shape, the elastic modulus of the balloon type femoral head is equivalent to that of the bone cartilage of the acetabulum, the balloon type femoral head is beneficial to cartilage nutrition, and the wear of the bone cartilage of the acetabulum is small; the balloon is in a shriveled state when no filler is filled in the balloon, the balloon can be placed in the femur along the sleeve, the joint capsule and the medullary cavity do not need to be opened, the trauma is small, the risk of subsequent infection and dislocation is small, meanwhile, the prosthesis wear can be replaced through the sleeve, and the replacement is convenient and fast and can be completed under local anesthesia.

The invention discloses an osteochondral integrated sponge scaffold as well as a preparation method and application thereof, and belongs to the field of osteochondral repair materials. The preparation method comprises the following steps: (1) mixing a collagen dispersion liquid and a polyvinyl alcohol solution, then adding a phosphate buffer solution and beta-tricalcium phosphate, and then obtaining lower-layer hydrogel through a pouring method; (2) mixing the collagen dispersion liquid with a polyvinyl alcohol solution, then adding a phosphate buffer solution, and then obtaining upper-layer hydrogel through a pouring method; and (3) pouring the upper-layer hydrogel on the lower-layer hydrogel, constructing a sponge scaffold through a multi-time freeze-thawing method and a freeze-drying method, and finally soaking the sponge scaffold in thrombin to obtain the osteochondral integrated sponge scaffold. The scaffold can adsorb platelet-rich plasma, has certain adhesion capacity, can realize in-situ fixation and biological integration at a defect part, can well recover physical and biological dysfunctions of bone joints and osteochondral bones, and provides a potential application idea for clinical treatment of osteochondral defects.

The application relates to the field of biological medicines, in particular to a cartilage tissue repair hydrogel and a preparation method thereof. The preparation components of the cartilage tissue repair hydrogel are doped with 10-15 g of mulberry silk, 0.1-0.5 g of glycidyl methacrylate, 0-1 g of lithium magnesium silicate, 0-1 g of tannic acid and 0.1 g of a photo initiator per 100 ml of the hydrogel. The preparation method of the cartilage tissue repair hydrogel is as follows: degumming and dissolving treatment are conducted on the mulberry silk to obtain a silk fibroin solution, glycidyl methacrylate is added into the silk fibroin solution and stirring is conducted to obtain a methacrylated silk fibroin solution, lithium magnesium silicate solution and tannic acid solution are added into the methacrylated silk fibroin solution to obtain a silk fibroin mixed solution, and then the photo initiator is added into the silk fibroin mixed solution to prepare the cartilage tissue repair hydrogel. The tissue repair hydrogel has a hydrophilic three-dimensional network structure, can provide mechanical support and biological induction activity for a bone cartilage defect site, and is suitable for bone injury repair.

The application discloses a kind of osteochondral double-layer composite scaffold and its preparation method, wherein, osteochondral double-layer composite scaffold includes the three-dimensional porous ceramic scaffold of 3D printing integrated molding and the cartilage layer, hard bone layer being arranged on three-dimensional porous ceramic scaffold, in the application embodiment, using the above-mentioned one osteochondral double-layer composite scaffold and its preparation method, using the hard support structure of porous ceramic, respectively adding carrier in porous upper support layer, porous lower support layer and loading / bioactive substance filling, can realize bone cartilage integrated repair, can also solve the problem that flexible cartilage repair support is excessively compressed in the process of surgical implantation flexible cartilage repair support and leads to growth factor from carrier leakage, realize the slow release of bioactive substance after double-layer support is implanted, promote the differentiation of autologous bone marrow mesenchymal stem cells, and the through-hole structure in support interior, it is beneficial to the attachment of composite hydrogel and mesoporous bioactive glass, also beneficial to the adhesion and crawling of cell in support interior after implantation in vivo, promote new bone tissue.

The invention relates to an osteochondral syringe type adsorption pen. The osteochondral syringe type adsorption pen comprises an adsorption sleeve with an inner cavity, an adsorption assembly connected with one end of the adsorption sleeve and an operating rod. The adsorption assembly is provided with adsorption holes communicated with the inner cavity, and the front end face of the adsorption assembly is an arc-shaped adsorption face. Two opposite ends of the operating rod are respectively marked as a first end extending into the inner cavity and a second end located outside the inner cavity and provided with a pressing part, and the first end is provided with a piston head matched with the inner wall of the inner cavity; an elastic device is arranged between the operating rod and the adsorption sleeve, and the operating rod overcomes the elastic force of the elastic device when bearing external force and drives the piston head to move towards one side of the adsorption assembly. And when the external force is removed, the piston head is driven to move towards one side far away from the adsorption assembly under the action of the restoring force of the elastic device. Non-contact operation in the whole process of graft obtaining and transplantation is achieved, edge damage is avoided, and structural integrity is guaranteed. Meanwhile, the obtaining and transplanting integrated function is achieved, surgical instrument replacement is reduced, and the pollution risk is reduced.

The invention relates to the field of tissue engineering biological materials, in particular to a preparation method and application of keratin-based composite osteochondral, and according to the preparation method, a cartilage layer 1, a calcified cartilage layer 2 and a subchondral bone layer 3 are combined into the composite osteochondral. The cartilage layer 1 is keratin-based nanofiber aerogel prepared through an electrostatic spinning method and a foaming technology, the calcified cartilage layer 2 is a keratin nanofiber membrane which is prepared through the electrostatic spinning method and is rich in calcium and phosphorus ions, and the subchondral bone layer 3 is a modified keratin-based hydrogel material prepared through a 3D printing technology. The three layers of structures are combined in sequence to form the keratin-based composite osteochondral bone. The prepared keratin-based scaffold has good biocompatibility, is beneficial to adhesion and proliferation of bone cells, and has the function of promoting repair and regeneration of bone cartilage.

The invention relates to the field of biomedical materials, and particularly discloses a novel collagen-calcium phosphorus-based flexible ceramic bone cartilage restoration and a preparation method thereof. The novel collagen-calcium phosphorus-based flexible ceramic bone cartilage restoration comprises a calcium phosphorus-based flexible ceramic bone phase layer which takes beta-tricalcium phosphate ceramic microspheres and monocalcium phosphate powder as solid phase components and takes distilled water or pure water, sodium hydroxide and disodium dihydrogen pyrophosphate as liquid phase components; at least one anchoring port is arranged on the top surface of the bone phase layer; and the collagen porous cartilage layer is formed through 3D printing by using printing ink prepared from a collagen solution and a photocuring system. According to the novel collagen-calcium phosphorus-based flexible ceramic bone cartilage restoration and the preparation method thereof, compared with the prior art, substantial improvement is achieved in the aspects of structural design, interface bonding, mechanical property, clinical operability, restoration effect and the like, and integrated innovation of flexible ceramic-collagen-anchoring structure-3D printing is embodied.

The invention discloses an enzyme response self-assembly polypeptide modified methacrylated polymer as well as a preparation method and application thereof. The enzyme response self-assembly polypeptide modified methacrylated polymer has a structure as shown in a formula 1. The concentration gradient of the endogenous tissue specific marker alkaline phosphatase is converted into dynamic gradient evolution of the mechanical property of the stent. Enzyme-responsive self-assembled polypeptide is introduced into a methacrylated macromolecular structure, so that the scaffold is subjected to molecular-level dynamic self-assembly under the catalysis of phosphatase, and a continuous mechanical gradient is formed from a cartilage region to a subchondral bone region. The process does not need external intervention and is completely regulated and controlled by a local microenvironment, so that the dynamic requirements of early proliferation-late differentiation of osteochondral repair are met in real time. Compared with the prior art, the method has the advantages that the problem of mismatch between static gradient and dynamic microenvironment is solved, mechanical signals are accurately regulated and controlled through molecular scale, and space specificity and time synchronism of stem cell differentiation are remarkably improved.

The osteochondral graft centrifugal device comprises a centrifugal tube and a separation assembly located in the centrifugal tube, the separation assembly comprises a cylindrical first support and a cylindrical second support, the outer diameter of the first support is matched with the inner diameter of the centrifugal tube, and the second support is far away from the open end of the centrifugal tube relative to the first support and is connected with the first support; the outer diameter of the second support is smaller than the inner diameter of the first support, an annular gap is formed between the second support and the inner wall of the centrifugal tube, a step face is formed in the connecting position of the second support and the first support, and a first through notch is formed in the step face. According to the osteochondral graft centrifugal device, the separating assembly is arranged in the centrifugal tube to bear the osteochondral graft to be treated, the articular cartilage and bone tissue below the articular cartilage can be protected, and meanwhile the purpose of separating bone marrow in the osteochondral graft to be treated is achieved.

The application provides a kind of osteochondral tissue repair scaffold and its preparation method.The osteochondral tissue repair scaffold is made by self-made photo-curing 3D printing equipment integration, including bionic double-layer structure: cartilage layer and subchondral bone layer, cartilage layer has "lotus root-like" and "radiation-like" hole, subchondral bone layer has "lotus root-like" hole, and longitudinal interstices between cartilage layer and subchondral bone layer are mutually through, aperture is accurately controlled, and also can add calcium phosphate nanocluster material with 1 nanometer particle size in subchondral bone layer.The osteochondral tissue repair scaffold prepared by the application aims at the different tissue characteristics and regeneration requirements of cartilage and subchondral bone, designs double-layer scaffold with different physical and biological characteristics, simulates the anisotropy of natural osteochondral structure, promotes cell migration and tissue growth, and achieves the effect of promoting synchronous regeneration of cartilage and subchondral bone, so it has great application potential.

The application provides a kind of osteochondral repair material and its preparation method and application, belong to the technical field of biomedical materials.The application is prepared hyaluronic acid-collagen with collagen as organic matrix, through the crosslinking reaction mediated by crosslinking agent;Hydroxyapatite is deposited in situ in the self-assembly process of collagen fiber through in vitro biomimetic mineralization technology, and mineralized collagen with different mineralization degrees is prepared;Iterative layered freeze-drying technology is used to construct biomimetic cartilage layer, calcification layer and subchondral layer in turn, and transforming growth factor-beta 3 and platelet-derived growth factor-BB are also loaded by physical adsorption to synergistically promote the repair and regeneration of osteochondral defects.The preparation process of the application is mild and controllable, and the prepared osteochondral repair material has good biocompatibility and intelligent active factor delivery function, and has important application prospect in the field of osteochondral tissue engineering and clinical defect repair.

The invention belongs to the technical field of biomedical materials, and particularly relates to a highly-bionic gradient artificial cartilage scaffold material, a preparation method and application, the highly-bionic gradient artificial cartilage scaffold material comprises a hyaline cartilage layer, a calcified cartilage layer and a subchondral bone layer, the calcified cartilage layer is composed of mineralized I-type collagen fibers containing 30% of nano-hydroxyapatite, and the subchondral bone layer is composed of mineralized I-type collagen fibers containing 60% of nano-hydroxyapatite; the prepared scaffold highly simulates natural osteochondral tissues, has similar composition, nano structure and mechanical properties with the natural osteochondral tissues, and presents an obvious gradient; the hydrogel has excellent biocompatibility and biological activity, and cell proliferation and gradient differentiation of bone marrow mesenchymal stem cells can be remarkably promoted; in a rabbit osteochondral defect model, osteochondral tissue regeneration is remarkably promoted, and the compound can be applied to clinical treatment of osteoarthritis.

The application relates to the field of biomedical materials, and particularly discloses a collagen-calcium-phosphorus-based flexible ceramic bone cartilage repair body and a preparation method thereof. The collagen-calcium-phosphorus-based flexible ceramic bone cartilage repair body comprises a calcium-phosphorus-based flexible ceramic bone phase layer, wherein beta-tricalcium phosphate ceramic microspheres and calcium phosphate powder are used as solid phase components, and distilled water or pure water, sodium hydroxide and disodium hydrogen pyrophosphate are used as liquid phase components; at least one anchoring harbor is arranged on the top surface of the bone phase layer; and a collagen porous cartilage layer is prepared by using a collagen solution and a photocuring system to prepare printing ink and then performing 3D printing. The collagen-calcium-phosphorus-based flexible ceramic bone cartilage repair body and the preparation method thereof are substantially improved in structural design, interface combination, mechanical properties, clinical operability and repair effect, and embody the integrated innovation of "flexible ceramic-collagen-anchoring structure-3D printing".