54 results about "Bones cartilage" patented technology

The present invention discloses process of preparing integral bionic tissue engineering bone-cartilage rack with functional interface. The process includes the first establishment of CAD model of the integral bone-cartilage rack by means of bionic principle; preparing collagen / chitosan and collagen / hydroxyapatite micro polymer powder; converting the CAD model into fast forming file input into a 3D printing fast forming machine, spreading the powder with one powder spreader, spraying pentanedial adhesive selectively on the spread powder for adhering and eliminating excessive powder to obtain the integral bionic tissue engineering bone-cartilage rack with functional interface. The present invention has excellent biocompatibility, controllable cartilage rack degrading speed and mechanical strength and other advantages.

Phototherapy apparatus, incorporating interconnected radiation sources, such as diode laser cluster radiation devices, and method for bone healing, bone growth stimulation, and bone cartilage regeneration are disclosed. The method consists of applying the said radiation cluster apparatus conformally around the desired area of the bone to be treated and providing irradiation at appropriate wavelengths and power densities for a selected period of time to the said area of the bone structure to be treated. The apparatus incorporates a sufficient number of the diode laser cluster devices, or other appropriate light sources, which are adapted to be placed inside an appropriate brace (e.g., ankle brace or knee brace), or are embedded inside a reconfigurable foam, or are embedded inside deformable gel material, or are embedded within the cast, which facilitate radially-positioned sources for irradiation of the area to be treated.

A cartilage therapeutic composition is developed for clinical transplantation into articulatio genu (knee joints) or ankle joints. It has clinical significance for symptomatic cartilage defects of the femoral condyle (medial, lateral, or trochlear) and bone cartilage defects of the talus (anklebone) in human or animal hosts, The cartilage therapeutic composition comprises a mixture of components of chondrocytes isolated and expanded or differentiated from a host such as a human or animal, and thrombin and a fibrinogen matrix containing fibrinogen. An application of the cartilage therapeutic composition is that a mixture of thrombin, chondrocyte components and a fibrinogen matrix is injected into a cartilage defect region followed by solidification therein. It provides rapid healing and effective regeneration of cartilage without surgical operation. It has the merits of safety and simplicity by allowing the use of an arthroscope for transplantation.

The invention discloses a tissue engineering bone cartilage composite bracket and an integrated photocuringable forming method thereof. The tissue engineering bone cartilage composite bracket serves as a ceramic bracket, wherein the ceramic bracket is served as a bone-repair part, and a porous structure is arranged on the surface of the ceramic bracketbone-repair part of the porous structure; and the ceramic bracket is fixed on a supporting plate of a photocuringable quick forming machine. A laser scanning path is driven through a computer aided design (CAD) model; and laser directly exposes and cures a hydrogel bracket and a pure hydrogel bracket which contain a ceramic component onto the ceramic bracket sequentially to form a three-layer composite bracket. Therefore, quick and fine preparation of a three-dimensional composite bracket of bone cartilage-like tissue histomorphometry is realized; uncontrollable structures and low efficiency because of handwork in the preparation process of a traditional composite bracket are avoided; the preparation efficiency is improved; and a calcified cartilage-like layer bracket and a hydrogel cartilage bracket with subtle structures can be bonded and compounded on the surface of the ceramic bracket by means of a rivet and a two-phase material.

The invention discloses a bone cartilage repair material and a preparation method of a scaffold for tissue engineering. The material comprises a subchondral bone layer, an interface layer and a cartilage layer arranged in sequence, wherein the interface layer is positioned between the subchondral bone layer and the cartilage layer; the material contains the following components in parts by weight:10-40 parts of oil-soluble high polymer materials, 10-50 parts of biological ceramic powder, 50-100 parts of oily solvents, 0.1-1 part of a water-soluble bio-active material, 2-30 parts of water, more than 0 and less than 1 part of an emulsifier, 10-30 parts of a hydrogel material, 0-10 parts of seed cells and 50-100 parts of a culture medium. The preparation method comprises the following steps:preparing printing ink according to the ratio of the materials, and transferring into 3D printing equipment for printing molding. The bone cartilage scaffold having a personalized appearance can be prepared, load and controlled release of different growth factors or peptides in different zones of the scaffold can be realized, and directional differentiation of bone marrow stem cells at differentparts to osteoblast and chondrocyte can be promoted.

The invention discloses a method for preparing functional gradient hydrogel for bone-cartilage repair and belongs to the technical field of biological materials. Two parts of double-bond biomacromolecule pre-polymerization liquid with the same mass fraction are prepared firstly, then the pre-polymerization liquid is prepared into upper-layer cartilage repair pre-polymerization liquid containing repair factor nanoparticles and lower-layer subchondral bone repair pre-polymerization liquid containing calcium phosphorus nanoparticles, and based on suspension principle and optical polymerization reaction, the functional gradient hydrogel is formed through sedimentation and diffusion of nanoparticles, wherein the function of the functional gradient hydrogel changes along with the change of compositions and structure. The prepared bionic hydrogel has high biodegradability and biocompatibility, loaded growth factors are high in efficiency and slow release performance, cartilage and subchondral bone repair can be induced, bidirectional regeneration of bone-cartilage is achieved, and repair of cartilago articularis injuries is achieved.

The invention provides an integrated bone-cartilage repair scaffold. The integrated bone-cartilage repair scaffold is of an integrated structure composed of a subchondral bone repair layer, a middlelayer and a cartilage repair layer, wherein the subchondral bone repair layer is fashioned porous calcium phosphate biological ceramic, the middle layer is sulfydryl-hyaluronic acid hydrogel, the cartilage repair layer is composed of I-type collagen hydrogel and chondrocytes or mesenchymal stem cells, and the middle layer is located between the subchondral bone repair layer and the cartilage repair layer and in a porous structure of the fashioned porous calcium phosphate biological ceramic to separate the subchondral bone repair layer from the cartilage repair layer. The invention further provides a preparation method of the integrated bone-cartilage repair scaffold. The repair scaffold can prevent blood vessels from invading a cartilage layer, so that the tightness and the stability of connection between a bone layer and the cartilage layer are improved, biological bonding of the bone layer and the cartilage layer is achieved, and thus a good repair effect is achieved.

The present invention relates to a complex support body for regenerating bone-cartilage, a method for manufacturing thereof, and a composition for treating bone and cartilage related diseases comprising the same as an active ingredient, and more particularly, to a complex support body for regenerating bone-cartilage, which comprises a bone regeneration layer consisting of a biodegradable polymer and a biocompatible ceramic, and a cartilage regeneration layer, which is coupled to the bone regeneration layer and in which cells that can be differentiated into cartilages cells are fixed; a method for manufacturing thereof; and a composition for treating bone and cartilage related diseases comprising the same as an active ingredient. The complex support body of the present invention for regenerating bone-cartilage is manufactured as a three-dimensional support body, which is similar to a living tissue, according to a bioplotting method, and exerts the effect of regeneration into a bone tissue and a cartilage tissue, respectively, depending on materials encountered in the environment where the complex support body for regenerating bone-cartilage is used.

The invention belongs to the field of cartilage tissue repair and tissue engineering and provides a cartilage complex of a tissue engineering bone and a method for preparing the same. The preparation method comprises the following steps of: performing marrow removal, degreasing and layered calcium-removal of cancellous allograft bones or xenogeneic cancellous bones to obtain the cartilage framework of tissue engineering bones, wherein the upper layer of the cartilage framework is completely decalcified and only the surface of the lower layer is decalcified; and using the framework as a primary framework, adding gel-type matters, such as fibrinogens or alginic acid, serving as a secondary framework and a distribution carrier to obtain the cartilage complex of the tissue engineering bone. Compared with the conventional tissue engineering bone cartilage, the cartilage complex of the invention has the advantages of complete natural biomaterials, complete integration, no application of the layered stacking and interface combination technology, high pressure resistant, tensile resistant and shearing resistant properties, uniform and accurate loading of cells, effective enforcement of the differentiation of seeded cells into cartilage cells, easy operation, convenient minimally invasive operation under an arthroscope, and the like.

The invention discloses a nanofiber bone cartilage repairing stent for tissue engineering and a preparation method thereof. The nanofiber bone cartilage repairing stent for tissue engineering is characterized by comprising a porous cartilage layer, a porous cartilage lower bone layer and a connecting layer between the porous cartilage layer and the porous cartilage lower bone layer, wherein the porous cartilage layer includes a PLLA-based composite degradable polymer material and is of a porous nanofiber structure which is 20-400mu m in pore diameter; the connecting layer includes a PLLA-based composite degradable polymer material and is of a porous nanofiber structure which is less than 5mu m in pore diameter; and the porous cartilage lower bone layer comprises a PLLA-based composite degradable polymer material containing 5-70% (w / w) of nano-hydroxyapatite and is of a porous nanofiber structure which is 50-500mu m in pore diameter. Microstructure of the bone cartilage repairing stent prepared by the invention is represented as bionic ECM nanofiber; the repairing stent can promote adhesion, propagation and differentiation of related bone cartilage cells on the stent, and can also promote transportation of nutrient substances in the stent and discharge of metabolic waste.

The invention discloses a collagen basal bone cartilage three-layer compound and a preparation method of the compound. The compound is prepared with the following method: sequentially injecting collagen-phosphate mixed solution, 5-20mg / mL of collagen water solution and 35-45mg / mL of collagen water solution into a mold; forming a dense collagen - multiple orifice collagen - collagen water soluble phosphoric acid compound; and after the compound is immerged by absolute ethyl alcohol, and absolute ethyl alcohol of calcium salt, crosslinking the compound by using the MES solution of EDC-NHS to obtain the collagen basal bone cartilage three-layer compound. The preparation method disclosed by the invention improves the interface bonding property of a three-layer bracket, and prevents the unfavorable crack consequence between layers occurred during the cartilage renovation process.

A tissue-engineered bone-cartilage assembly for repairing the damaged bone and cartilage by inserting it in the damaged position is prepared through preparing bone and cartilage by tissue engineering, adhering them together, and making a wedge on bone part.

The invention discloses a method for preparing a double-layer bionic cartilage tissue engineering scaffold, which comprises the following steps of: firstly, preparing a substrate; secondly, preparing the double-layer bionic cartilage tissue engineering scaffold on the surface of the substrate; and finally, soaking the double-layer bionic cartilage scaffold on the surface of the substrate by usingsolution of NaOH, and peeling the double-layer bionic cartilage scaffold from the substrate. The method has simple operation and high stability; the prepared double-layer bionic cartilage scaffold has a thin thickness and good mechanical properties and is favorable for clinical application; the scaffold is molded integrally; a vertical hole is tightly connected with a round hole to avoid the problem of breaking away from each other; the structure of the scaffold is similar to a natural cartilage structure and is hopeful to better maintain the phenotype of cartilage cells and improve the repairing effect on cartilage injuries; and the cartilage scaffold can be directly combined with a substrate comprising polylactic acid (PLA) / bone powder to form a bone cartilage scaffold used for repairing bone cartilage simultaneous colobomata, and a cartilage layer part still has a double-layer structure.

The invention discloses a method for culturing an artificial cartilage with different curved surfaces or a bone cartilage under the action of rolling load and sliding load. The method comprises an incubator which contains O2 and CO2 and can perform temperature adjustment and cell culture, perfusion and other conditions which are used for keeping culture solution fresh, and active factors which are added to the culture solution. The method is characterized in that culture blanks with different curved surfaces are fixed on the bottom plate of a culture room; during the culturing process, a culture is subjected to the effect of the rolling load and the sliding load simultaneously, and the culture is cultured into cartilage tissues or bone cartilage complex tissues. The method has the advantages that under the effect of the rolling load and the sliding load, the loading way of the method is closer to the mechanical environment of the growth and the development of the cartilage tissues; and under the loading condition, the method is favor of the growth and the development of cells and tissues. An experiment shows that mucopolysaccharide secreted by cartilage cells under the effect of the rolling load and the sliding load is higher than the mucopolysaccharide secreted by the cartilage cells only under the effect of the rolling load. After being cultured, the cultures with different shapes can be taken as implants, and the method of the invention is applied in repairing the defect of the cartilage in different parts.

The invention belongs to the field of cartilage tissue repair and tissue engineering and provides a tissue engineered bone cartilage frame and a preparation method thereof. Particularly, marrow removal and degreasing are carried out on allogeneic or xenogeneic cancellous bones to obtain cancellous bone columns with experimentally or clinically required diameters, and then a tissue engineered bonecartilage frame with a completely decalcified upper layer and a superficially decalcified lower layer is obtained via layered decalcification. The upper layer of the frame is used as the framework part of the tissue engineered cartilage frame or an engineering cartilage frame, and the lower layer thereof can be used as a bone forming frame, an engineering bone bracket and the fixed pivot of the engineered cartilage, and can be used for constructing an integrative tissue engineered bone cartilage or directly transplanted for the cartilage or bone cartilage repair. Compared with the existing tissue engineered bone cartilage frame, the invention has the advantages of integration (no interface mechanical defect), and good compatibility of a pore structure and the tissue, has better compression resisting, tensile resisting and shear resisting properties, and is convenient for the minimally invasive surgery operation under an arthroscope.

The invention belongs to the technical field of biomedical materials and discloses a bi-phase bone cartilage repairing support and a preparing method thereof.The preparing method comprises the steps that chitosan and hyaluronic acid are modified to obtain carboxyethyl-chitosan and oxidation hyaluronic acid, carboxyethyl-chitosan and oxidation hyaluronic acid are solved in a PBS buffer solution, then the obtained two solutions are mixed to obtain uniform cartilage layer hydrogel, nano-hydroxyapatite is added into the carboxyethyl-chitosan solution and the oxidation hyaluronic acid solution, and after ultrasonic dispersion and uniforming, the two solutions are mixed to obtain the uniform subchondral bone layer hydrogel; finally, the cartilage layer hydrogel and the subchondral bone layer hydrogel are transversely incised, new interfaces of the two kinds of hydrogel are glued to obtain the bi-phase bone cartilage repairing support.According to the preparing method, the biocompatibility of the adopted materials is good, operation is easy, and the cartilage layer hydrogel and the subchondral bone layer hydrogel can be firmly combined.

The invention belongs to the technical field of tissue engineering and gene delivery, in particular to a double-gene activated bone-cartilage compound transplant, a preparation method and application thereof. In the method, seed cells are respectively planted on two layers of bracket materials which are activated by different genes and have the same main constituents, and the seed cells of the upper layer and the lower layer are simultaneously induced to respectively differentiate to chondrocyte and osteoblast by utilizing an in situ gene transfection principle so as to form a tissue engineering bone-cartilage compound transplant in vitro, wherein the seed cells are bone mesenchymal stem cells; the main constituents of the bracket materials are chitosan and gelatin; and in the two different genes, one is a gene differentiated from any known induced stem cell to the chondrocyte, while the other one is a gene differentiated from any known induced stem cell to the osteoblast. By utilizing the method, the bone-cartilage compound transplant is prepared in vitro and further used for restoring bone-cartilage.

The invention provides a method for preparing an integration bone cartilage stent based on a 3DP and laser cladding compound process. The 3DP technology is adopted for preparing a stent cartilage lower bone layer, the laser cladding technology is adopted for preparing a cartilage layer, the 3DP and laser cladding technology is adopted for preparing a stent calcification layer, the requirement forthe biocompatibility of the bone cartilage is met while precise preparation of the integration bone cartilage stent is achieved, the requirement for the connecting strength and mechanical strength among all layers of tissue of the stent is met, and the integration bone cartilage stent which meets the structural gradient and mechanical gradient bionics is prepared.

The invention discloses a bone-cartilage bidirectional function bracket based on cell 3D printing, and a preparation method thereof. The preparation method for the bone-cartilage bidirectional function bracket based on cell 3D printing comprises the following steps: S1: respectively preparing an osteogenesis ink substrate and a chondrogenesis ink substrate; S2: adding mesenchymal stem cells and naringin into the osteogenesis ink substrate to obtain osteogenesis ink, and adding the mesenchymal stem cells and a transforming growth factor beta into the chondrogenesis ink substrate to obtain chondrogenesis ink; S3: respectively printing the osteogenesis ink and the chondrogenesis ink to obtain a bracket, wherein the bracket comprises an osteogenesis side and a chondrogenesis side; S4: after the bracket is subjected to crosslinking treatment, obtaining the bone-cartilage bidirectional function bracket. According to the bone-cartilage bidirectional function bracket prepared with the preparation method, the combined regeneration of bone-cartilage can be simultaneously realized, personalized customization is realized, the bone-cartilage bidirectional function bracket is favorable for the tissue regeneration of joints, and therefore, damaged joints can be more favorably repaired.

The invention belongs to the technical field of 3D printing materials, and particularly relates to a composite osteochondral stent as well as a preparation method and application thereof. The invention provides a composite bone cartilage scaffold. The composite bone cartilage scaffold sequentially comprises a subchondral bone layer, a fibrous membrane-shaped transition layer and a cartilage layer with a net structure from bottom to top; the subchondral bone layer is made of polyether-ether-ketone, and the surface of the subchondral bone layer is coated with a biocompatible layer; the material of the cartilage layer comprises a cartilage matrix and biological factors, and the cartilage matrix is a hydrogel material; and the cartilage layer contains biological factors. Biological factors contained in the cartilage layer are beneficial to the growth of cartilage; The transition layer can realize the exchange of nutrient substances between the cartilage layer and the subchondral bone layer, and meanwhile, the cartilage layer and the subchondral bone layer have relatively independent development environments, so that the problem of fibrosis is avoided. The composite osteochondral scaffold provided by the invention can be well integrated with surrounding tissues, and integrated repair of cartilage and subchondral bone can be realized.

The invention belongs to the technical field of medical treatment, and particularly relates to a tendon-bone combined three-phase scaffold prepared by fusion electrospinning three-dimensional printing. A method comprises the following steps: S1, cell cultivation; S2, bracket preparation; and S3, cell planting. The method can accurately control the fiber diameter and the printing path by using a fusion electrostatic spinning three-dimensional printing technology, thereby greatly improving the printing precision; by using a spinning solution containing cytokine microspheres for electrospinning printing, the electrospinning fibers containing a large amount of cytokines can be obtained; and through different three-dimensional printing path parameters, the tendon-cartilage-bone three-phase scaffold can be obtained by printing. The scaffold obtained by the method has a bone-cartilage-tendon tissue gradient structure, has high structure precision, carries a large amount of microspheres with cytokines for slow release, promotes cell proliferation repair, and is beneficial to tendon-bone combined and normal function recovery.

The invention discloses a tissue engineering composite stent for osteochondral repair. The stent is obtained by adding a cell suspension dropwise to a cell-porous material composite and then conducting gelating. The cell-adhesive porous stent is a porous stent containing collagen, gelatin, fibronectin or cell-adhesive polypeptide, and all the materials are commonly used biomedical materials with good biocompatibility and are biodegradable in vivo. Hydrogel macromonomer sodium alginate, hyaluronic acid, agarose and chitosan also have good biocompatibility and biodegradability. Polyethylene glycol diacrylate is a biological material that is bio-inert and can be used in vivo, does not cause blood clotting reaction in vivo and is excreted through the kidneys. The composite stent can be used asa bone-cartilage tissue engineering / repair stent material, and has beneficial technical effects and wide application prospects.

The invention relates to a difunctional integrated bone-cartilage composite tissue engineering scaffold for clinic treatment of osteosarcoma. Firstly, polylactic acid / hydroxyapatite hard bone and calcium carbonate glucolactone / sodium alginate (CaCO3-GDL-SA) cartilage scaffolds are prepared by solvent casting-particle leaching and compound ion crosslinking network technologies respectively. The integrated bone-cartilage composite scaffold has bionic gradient change in physical and biological performance, is expected to fill various defects in irregular shapes and can effectively promote simultaneous generation of bone-cartilage tissue defects after osteosarcoma excision. CR780-PEG5K nanoparticles uniformly dispersed in the CaCO3-GDL-SA cartilage hydorgel scaffold can remove osteosarcoma cells under near infrared illumination on the basis of good photothermal effect. The difunctional integrated bone-cartilage composite scaffold provides theoretical basis and technical support for existing clinical tretament schemes of the osteosarcoma.

The invention provides a multifunctional telescopic cutting scalpel for the discoid meniscus operation under a knee arthroscope. The multifunctional telescopic cutting scalpel comprises a main sheath, an auxiliary sheath, a blade and a blade moving mechanism. The main sheath is provided with threaded holes, a button moving groove, a scaleplate scale, locking grooves and inclined lines. The auxiliary sheath is provided with threaded holes, a scaleplate scale and inclined lines. The blade is provided with a blade scale and a blade installing hole. The blade moving mechanism comprises a blade installing groove, an elastic steel sheet, a button, a locking pin and a blade holder. When reaching a tear portion from an inlet of the arthroscope, the cutting scalpel can effectively prevent damage to normal tissue such as peripheral vessels and nerves, thigh bone cartilage, tibial plateau cartilage and anterior and posterior cruciate ligaments; the multifunctional telescopic cutting scalpel can be used for measuring a fracture wound accurately, thereby guaranteeing accurate cutting and maintaining and protecting normal meniscus tissue to the greatest extent; the cutting scalpel is simple and convenient to operate, and the inclined lines can increase friction force to prevent the scalpel from falling off from hands; the locking device can prevent tissue damage caused by fault in gear change, thereby improving safety.

The invention relates to a calcium silicate lithium-system novel biological activity ceramic support and a preparation method and a purpose thereof. The calcium silicate lithium-system novel biological activity ceramic support is a three-dimensional porous support, and a chemical composition is LixCaySizOn, wherein x+2y+4z=2n. The calcium silicate lithium-system novel biological activity ceramic support has good degradability, mechanical properties and biological activity, has good bibiological activity, and can promote ossification and chondrogenic differentiation of mesenchymal stem cells and cartilage cells as well as in-vivo bone-cartilage integrated restoration.

The invention discloses a method for preparing a double-layer bionic cartilage tissue engineering scaffold, which comprises the following steps of: firstly, preparing a substrate; secondly, preparing the double-layer bionic cartilage tissue engineering scaffold on the surface of the substrate; and finally, soaking the double-layer bionic cartilage scaffold on the surface of the substrate by using solution of NaOH, and peeling the double-layer bionic cartilage scaffold from the substrate. The method has simple operation and high stability; the prepared double-layer bionic cartilage scaffold has a thin thickness and good mechanical properties and is favorable for clinical application; the scaffold is molded integrally; a vertical hole is tightly connected with a round hole to avoid the problem of breaking away from each other; the structure of the scaffold is similar to a natural cartilage structure and is hopeful to better maintain the phenotype of cartilage cells and improve the repairing effect on cartilage injuries; and the cartilage scaffold can be directly combined with a substrate comprising polylactic acid (PLA) / bone powder to form a bone cartilage scaffold used for repairing bone cartilage simultaneous colobomata, and a cartilage layer part still has a double-layer structure.

The invention discloses a production method of a calcified layer-containing bionic tissue engineering bone cartilage integrated support. The method comprises the following steps: carrying out vacuum hot pressing on a produced transparent cartilage layer, a calcified cartilage layer and a subchondral bone layer, and carrying out particle leaching to produce the integrated composite material support with the middle being the dense calcified cartilage layer and with two sides being the porous transparent cartilage layer and the subchondral bone layer respectively, wherein the transparent cartilage layer and the subchondral bone layer are obtained through a solvent casting technology with polylactic acid as a matrix, calcium polyphosphate short fibers as a reinforcement and sodium chloride as a pore forming agent; and the close calcified cartilage layer (CPPf / PLLA film) is produced through a film layer spreading technology with polylactic acid as a matrix and calcium polyphosphate long fibers as a reinforcement. The content of CPP fibers is controlled to increase the strength, the rigidity and the osteoinductivity of the CPPf / PLLA integrated porous support and control the degradation rate of the support in a simulation body fluid.