13 results about "Bones cartilage" patented technology

The invention relates to the technical field of biomedical materials, in particular to multi-material 3D printing bionic gradient soft-hard integrated hydrogel as well as a preparation method and application thereof. Based on a copolymerization system of high-strength supramolecular N-acryloylglycinamide and vinyl phosphoric acid, an NAGA aqueous solution and an NAGA-VPA aqueous solution are printed into hydrogel of a gradient structure by adopting a DLP multi-material printing technology, then the hydrogel is sequentially soaked in a calcium-phosphorus mixed aqueous solution (Ca / P = 1.67), ammonia water, a calcium chloride aqueous solution and deionized water, and the hydrogel is prepared through a hydrothermal method. Therefore, in-situ gradient deposition and curing of hydroxyapatite (HAP) are realized. The multi-material 3D printing bionic gradient soft-hard integrated hydrogel prepared by the method has a continuous biochemical-mechanical gradient, a surface layer low-modulus area is matched with cartilage performance, a deep layer high-mineralization area is integrated with bone tissue, the HAP crystallinity of a transition area is in spatial dependence distribution, and the hydrogel is suitable for osteochondral defect repair and manufacturing of bionic joint prostheses.

The invention relates to the technical field of biomedical materials, and provides a preparation method of a fibroin nanofiber reinforced silk protein gel material, the gel material and application, fibroin nanofibrils are prepared through a mechanical grinding method and introduced into composite hydrogel, the Young modulus reaches 4.68 MPa, and the fibroin nanofiber reinforced silk protein gel material is suitable for osteochondral repair and drug delivery.

The application discloses an enzyme response self-assembly polypeptide modified methacrylating polymer and a preparation method and application thereof, and the enzyme response self-assembly polypeptide modified methacrylating polymer has a structure of formula 1. The application converts an endogenous tissue-specific marker alkaline phosphatase concentration gradient into a dynamic gradient evolution of the mechanical properties of a scaffold. By introducing an enzyme response self-assembly polypeptide into a methacrylating polymer structure, the scaffold is subjected to molecular level dynamic self-assembly under the catalysis of phosphatase, and a continuous mechanical gradient is formed from a cartilage region to a subchondral bone region. The process does not require external intervention and is completely regulated by a local microenvironment, thereby real-time adapting to the dynamic requirements of 'early proliferation-late differentiation' of bone cartilage repair. Compared with the prior art, the application not only breaks through the mismatch between a static gradient and a dynamic microenvironment, but also precisely regulates mechanical signals at a molecular scale, and significantly improves the spatial specificity and time synchronism of stem cell differentiation.

The application relates to the technical field of femoral head necrosis treatment, in particular to a bionic artificial balloon type half hip joint, which comprises a sleeve, a femoral neck, a fixing screw and a femoral neck limiting nut, the sleeve is fixed on a femur through the fixing screw, the femoral neck is slidingly connected in the sleeve, a balloon is fixed on the outer periphery of the top section of the femoral neck, the inside of the femoral neck is hollow, and a side hole is arranged at the top end of the femoral neck and communicates with the balloon, and the femoral neck limiting nut is used for being fixed in the sleeve to position the femoral neck. The balloon type femoral head is compared with the metal and ceramic femoral heads, the balloon type femoral head is more matched with the shape of the acetabulum in terms of shape, the elastic modulus of the balloon type femoral head is equivalent to that of the bone cartilage of the acetabulum, the balloon type femoral head is beneficial to cartilage nutrition, and the wear of the bone cartilage of the acetabulum is small; the balloon is in a shriveled state when no filler is filled in the balloon, the balloon can be placed in the femur along the sleeve, the joint capsule and the medullary cavity do not need to be opened, the trauma is small, the risk of subsequent infection and dislocation is small, meanwhile, the prosthesis wear can be replaced through the sleeve, and the replacement is convenient and fast and can be completed under local anesthesia.

The invention discloses an osteochondral integrated sponge scaffold as well as a preparation method and application thereof, and belongs to the field of osteochondral repair materials. The preparation method comprises the following steps: (1) mixing a collagen dispersion liquid and a polyvinyl alcohol solution, then adding a phosphate buffer solution and beta-tricalcium phosphate, and then obtaining lower-layer hydrogel through a pouring method; (2) mixing the collagen dispersion liquid with a polyvinyl alcohol solution, then adding a phosphate buffer solution, and then obtaining upper-layer hydrogel through a pouring method; and (3) pouring the upper-layer hydrogel on the lower-layer hydrogel, constructing a sponge scaffold through a multi-time freeze-thawing method and a freeze-drying method, and finally soaking the sponge scaffold in thrombin to obtain the osteochondral integrated sponge scaffold. The scaffold can adsorb platelet-rich plasma, has certain adhesion capacity, can realize in-situ fixation and biological integration at a defect part, can well recover physical and biological dysfunctions of bone joints and osteochondral bones, and provides a potential application idea for clinical treatment of osteochondral defects.

The application discloses a kind of osteochondral double-layer composite scaffold and its preparation method, wherein, osteochondral double-layer composite scaffold includes the three-dimensional porous ceramic scaffold of 3D printing integrated molding and the cartilage layer, hard bone layer being arranged on three-dimensional porous ceramic scaffold, in the application embodiment, using the above-mentioned one osteochondral double-layer composite scaffold and its preparation method, using the hard support structure of porous ceramic, respectively adding carrier in porous upper support layer, porous lower support layer and loading / bioactive substance filling, can realize bone cartilage integrated repair, can also solve the problem that flexible cartilage repair support is excessively compressed in the process of surgical implantation flexible cartilage repair support and leads to growth factor from carrier leakage, realize the slow release of bioactive substance after double-layer support is implanted, promote the differentiation of autologous bone marrow mesenchymal stem cells, and the through-hole structure in support interior, it is beneficial to the attachment of composite hydrogel and mesoporous bioactive glass, also beneficial to the adhesion and crawling of cell in support interior after implantation in vivo, promote new bone tissue.

The invention relates to an osteochondral syringe type adsorption pen. The osteochondral syringe type adsorption pen comprises an adsorption sleeve with an inner cavity, an adsorption assembly connected with one end of the adsorption sleeve and an operating rod. The adsorption assembly is provided with adsorption holes communicated with the inner cavity, and the front end face of the adsorption assembly is an arc-shaped adsorption face. Two opposite ends of the operating rod are respectively marked as a first end extending into the inner cavity and a second end located outside the inner cavity and provided with a pressing part, and the first end is provided with a piston head matched with the inner wall of the inner cavity; an elastic device is arranged between the operating rod and the adsorption sleeve, and the operating rod overcomes the elastic force of the elastic device when bearing external force and drives the piston head to move towards one side of the adsorption assembly. And when the external force is removed, the piston head is driven to move towards one side far away from the adsorption assembly under the action of the restoring force of the elastic device. Non-contact operation in the whole process of graft obtaining and transplantation is achieved, edge damage is avoided, and structural integrity is guaranteed. Meanwhile, the obtaining and transplanting integrated function is achieved, surgical instrument replacement is reduced, and the pollution risk is reduced.

The invention relates to the field of biomedical materials, and particularly discloses a novel collagen-calcium phosphorus-based flexible ceramic bone cartilage restoration and a preparation method thereof. The novel collagen-calcium phosphorus-based flexible ceramic bone cartilage restoration comprises a calcium phosphorus-based flexible ceramic bone phase layer which takes beta-tricalcium phosphate ceramic microspheres and monocalcium phosphate powder as solid phase components and takes distilled water or pure water, sodium hydroxide and disodium dihydrogen pyrophosphate as liquid phase components; at least one anchoring port is arranged on the top surface of the bone phase layer; and the collagen porous cartilage layer is formed through 3D printing by using printing ink prepared from a collagen solution and a photocuring system. According to the novel collagen-calcium phosphorus-based flexible ceramic bone cartilage restoration and the preparation method thereof, compared with the prior art, substantial improvement is achieved in the aspects of structural design, interface bonding, mechanical property, clinical operability, restoration effect and the like, and integrated innovation of flexible ceramic-collagen-anchoring structure-3D printing is embodied.

The invention discloses an enzyme response self-assembly polypeptide modified methacrylated polymer as well as a preparation method and application thereof. The enzyme response self-assembly polypeptide modified methacrylated polymer has a structure as shown in a formula 1. The concentration gradient of the endogenous tissue specific marker alkaline phosphatase is converted into dynamic gradient evolution of the mechanical property of the stent. Enzyme-responsive self-assembled polypeptide is introduced into a methacrylated macromolecular structure, so that the scaffold is subjected to molecular-level dynamic self-assembly under the catalysis of phosphatase, and a continuous mechanical gradient is formed from a cartilage region to a subchondral bone region. The process does not need external intervention and is completely regulated and controlled by a local microenvironment, so that the dynamic requirements of early proliferation-late differentiation of osteochondral repair are met in real time. Compared with the prior art, the method has the advantages that the problem of mismatch between static gradient and dynamic microenvironment is solved, mechanical signals are accurately regulated and controlled through molecular scale, and space specificity and time synchronism of stem cell differentiation are remarkably improved.

The application provides a kind of osteochondral tissue repair scaffold and its preparation method.The osteochondral tissue repair scaffold is made by self-made photo-curing 3D printing equipment integration, including bionic double-layer structure: cartilage layer and subchondral bone layer, cartilage layer has "lotus root-like" and "radiation-like" hole, subchondral bone layer has "lotus root-like" hole, and longitudinal interstices between cartilage layer and subchondral bone layer are mutually through, aperture is accurately controlled, and also can add calcium phosphate nanocluster material with 1 nanometer particle size in subchondral bone layer.The osteochondral tissue repair scaffold prepared by the application aims at the different tissue characteristics and regeneration requirements of cartilage and subchondral bone, designs double-layer scaffold with different physical and biological characteristics, simulates the anisotropy of natural osteochondral structure, promotes cell migration and tissue growth, and achieves the effect of promoting synchronous regeneration of cartilage and subchondral bone, so it has great application potential.

The application provides a kind of osteochondral repair material and its preparation method and application, belong to the technical field of biomedical materials.The application is prepared hyaluronic acid-collagen with collagen as organic matrix, through the crosslinking reaction mediated by crosslinking agent;Hydroxyapatite is deposited in situ in the self-assembly process of collagen fiber through in vitro biomimetic mineralization technology, and mineralized collagen with different mineralization degrees is prepared;Iterative layered freeze-drying technology is used to construct biomimetic cartilage layer, calcification layer and subchondral layer in turn, and transforming growth factor-beta 3 and platelet-derived growth factor-BB are also loaded by physical adsorption to synergistically promote the repair and regeneration of osteochondral defects.The preparation process of the application is mild and controllable, and the prepared osteochondral repair material has good biocompatibility and intelligent active factor delivery function, and has important application prospect in the field of osteochondral tissue engineering and clinical defect repair.

The invention belongs to the technical field of biomedical materials, and particularly relates to a highly-bionic gradient artificial cartilage scaffold material, a preparation method and application, the highly-bionic gradient artificial cartilage scaffold material comprises a hyaline cartilage layer, a calcified cartilage layer and a subchondral bone layer, the calcified cartilage layer is composed of mineralized I-type collagen fibers containing 30% of nano-hydroxyapatite, and the subchondral bone layer is composed of mineralized I-type collagen fibers containing 60% of nano-hydroxyapatite; the prepared scaffold highly simulates natural osteochondral tissues, has similar composition, nano structure and mechanical properties with the natural osteochondral tissues, and presents an obvious gradient; the hydrogel has excellent biocompatibility and biological activity, and cell proliferation and gradient differentiation of bone marrow mesenchymal stem cells can be remarkably promoted; in a rabbit osteochondral defect model, osteochondral tissue regeneration is remarkably promoted, and the compound can be applied to clinical treatment of osteoarthritis.

The application relates to the field of biomedical materials, and particularly discloses a collagen-calcium-phosphorus-based flexible ceramic bone cartilage repair body and a preparation method thereof. The collagen-calcium-phosphorus-based flexible ceramic bone cartilage repair body comprises a calcium-phosphorus-based flexible ceramic bone phase layer, wherein beta-tricalcium phosphate ceramic microspheres and calcium phosphate powder are used as solid phase components, and distilled water or pure water, sodium hydroxide and disodium hydrogen pyrophosphate are used as liquid phase components; at least one anchoring harbor is arranged on the top surface of the bone phase layer; and a collagen porous cartilage layer is prepared by using a collagen solution and a photocuring system to prepare printing ink and then performing 3D printing. The collagen-calcium-phosphorus-based flexible ceramic bone cartilage repair body and the preparation method thereof are substantially improved in structural design, interface combination, mechanical properties, clinical operability and repair effect, and embody the integrated innovation of "flexible ceramic-collagen-anchoring structure-3D printing".