Difunctional integrated bone-cartilage composite tissue engineering scaffold for clinic treatment of osteosarcoma

A clinical treatment and composite tissue technology, applied in tissue regeneration, prosthesis, anti-tumor drugs, etc., to achieve the effect of promoting simultaneous regeneration, good photothermal effect, and improving interface compatibility

Active Publication Date: 2019-11-08
FUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the results reported in the current research belong to separate tumor models and bone defect models, such as the subcutaneous osteosarcoma model in nude mice and the rabbit femur / rat skull defect model, and are not based on a unified bone tumor model at the joint to evaluate the integration at the same time. Photothermal therapy of tumor and bone regeneration ability of bifunctional scaffolds

Method used

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  • Difunctional integrated bone-cartilage composite tissue engineering scaffold for clinic treatment of osteosarcoma
  • Difunctional integrated bone-cartilage composite tissue engineering scaffold for clinic treatment of osteosarcoma
  • Difunctional integrated bone-cartilage composite tissue engineering scaffold for clinic treatment of osteosarcoma

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Experimental program
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Effect test

Embodiment 1

[0052] (1) Prepare a certain concentration of PLLA / chloroform solution (8%, W / V ), then weigh a certain amount of HA (the mass fraction of HA in PLLA / chloroform solution is 15%, W / V )) dispersed in PLLA / chloroform solution, and ensure the uniform distribution of HA in the polymer matrix to obtain PLLA / HA solution;

[0053] (2) In order to prepare a hard bone scaffold with a certain pore size, NaCl particles with a weight ratio of NaCl to PLLA / HA solution of 6:1 were sieved (180-90 mesh), and then slowly poured into the PLLA / HA solution, using Stir quickly with a glass rod until uniform, then pour into homemade glass molds;

[0054] (3) Put the mold in a fume hood to exhaust for 12 h, then put it in a vacuum oven set at 60 °C to dry for 24 h, and finally soak the formed hard bone scaffold in deionized water for 7 days to complete particle leaching Hole making;

[0055] (4) In order to improve the interfacial compatibility of the composite scaffold, a certain mass of NaOH par...

Embodiment 2

[0071] (1) Prepare a certain concentration of PLLA / chloroform solution (12%, W / V ), then weighed a certain mass of HA (the mass fraction of HA in PLLA / chloroform solution was 25%, W / V ) is dispersed in PLLA / chloroform solution, and ensures the uniform distribution of HA in the polymer matrix to obtain PLLA / HA solution;

[0072] (2) In order to prepare a hard bone scaffold with a certain pore size, NaCl particles with a weight ratio of NaCl to PLLA / HA solution of 6:1 were sieved (180-90 mesh), and then slowly poured into the PLLA / HA solution, using Stir quickly with a glass rod until uniform, then pour into homemade glass molds;

[0073] (3) Put the mold in a fume hood to exhaust for 18 h, then dry it in a vacuum oven at 65 °C for 30 h, and finally soak the formed hard bone scaffold in deionized water for 10 days to complete particle leaching Hole making;

[0074] (4) In order to improve the interfacial compatibility of the composite scaffold, a certain mass of NaOH particle...

Embodiment 3

[0080] (1) Prepare a certain concentration of PLLA / chloroform solution (8%, W / V ), then weigh a certain amount of HA (the mass fraction of HA in PLLA / chloroform solution is 15%, W / V ) is dispersed in PLLA / chloroform solution, and ensures the uniform distribution of HA in the polymer matrix to obtain PLLA / HA solution;

[0081] (2) In order to prepare a hard bone scaffold with a certain pore size, NaCl particles with a weight ratio of NaCl to PLLA / HA solution of 7:1 were sieved (180-90 mesh), and then slowly poured into the PLLA / HA solution, Stir quickly with a glass rod until uniform, then pour into homemade glass molds;

[0082] (3) Put the mold in a fume hood to exhaust for 12 h, then put it in a vacuum oven set at 55 °C to dry for 12 h, and finally soak the formed hard bone scaffold in deionized water for 7 days to complete particle leaching Hole making;

[0083] (4) In order to improve the interfacial compatibility of the composite scaffold, a certain mass of NaOH partic...

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Abstract

The invention relates to a difunctional integrated bone-cartilage composite tissue engineering scaffold for clinic treatment of osteosarcoma. Firstly, polylactic acid / hydroxyapatite hard bone and calcium carbonate glucolactone / sodium alginate (CaCO3-GDL-SA) cartilage scaffolds are prepared by solvent casting-particle leaching and compound ion crosslinking network technologies respectively. The integrated bone-cartilage composite scaffold has bionic gradient change in physical and biological performance, is expected to fill various defects in irregular shapes and can effectively promote simultaneous generation of bone-cartilage tissue defects after osteosarcoma excision. CR780-PEG5K nanoparticles uniformly dispersed in the CaCO3-GDL-SA cartilage hydorgel scaffold can remove osteosarcoma cells under near infrared illumination on the basis of good photothermal effect. The difunctional integrated bone-cartilage composite scaffold provides theoretical basis and technical support for existing clinical tretament schemes of the osteosarcoma.

Description

technical field [0001] The invention belongs to the technical field of biomaterials, and in particular relates to a dual-functional integrated bone-cartilage composite tissue engineering support for clinical treatment of osteosarcoma. Background technique [0002] Osteosarcoma is the most common primary malignant bone tumor in young people, with aggressive biological characteristics, easy recurrence and high rate of early metastasis. Surgical resection of tumor tissue combined with multi-drug chemotherapy and radiotherapy can control tumor metastasis to a certain extent and improve survival rate. However, the poor prognosis and radioresistance caused by multidrug resistance mechanism make the clinical treatment of osteosarcoma extremely challenging. The past decade has witnessed the rapid development of nanotechnology in the fields of materials science, molecular pharmacy, biology and oncology. Rich therapeutic platforms based on nanomedicine and nanotherapeutics have emer...

Claims

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Application Information

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IPC IPC(8): A61L27/46A61L27/44A61L27/20A61L27/02A61L27/50A61L27/52A61L27/56A61K41/00A61P35/00
CPCA61K41/0052A61L27/025A61L27/20A61L27/446A61L27/46A61L27/50A61L27/52A61L27/56A61L2430/02A61L2430/06A61P35/00C08L67/04C08L5/04
Inventor 张进王君曾亮丹乔紫雯杨黄浩
Owner FUZHOU UNIV
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