Method for preparing double-layer bionic cartilage tissue engineering scaffold

Abstract

The invention discloses a method for preparing a double-layer bionic cartilage tissue engineering scaffold, which comprises the following steps of: firstly, preparing a substrate; secondly, preparing the double-layer bionic cartilage tissue engineering scaffold on the surface of the substrate; and finally, soaking the double-layer bionic cartilage scaffold on the surface of the substrate by using solution of NaOH, and peeling the double-layer bionic cartilage scaffold from the substrate. The method has simple operation and high stability; the prepared double-layer bionic cartilage scaffold has a thin thickness and good mechanical properties and is favorable for clinical application; the scaffold is molded integrally; a vertical hole is tightly connected with a round hole to avoid the problem of breaking away from each other; the structure of the scaffold is similar to a natural cartilage structure and is hopeful to better maintain the phenotype of cartilage cells and improve the repairing effect on cartilage injuries; and the cartilage scaffold can be directly combined with a substrate comprising polylactic acid (PLA) / bone powder to form a bone cartilage scaffold used for repairing bone cartilage simultaneous colobomata, and a cartilage layer part still has a double-layer structure.

Description

technical field

[0001] The invention relates to the technical field of scaffold preparation for cartilage tissue engineering, in particular to a preparation method for a double-layer bionic cartilage tissue engineering scaffold. Background technique

[0002] Due to its special position and function in the body, damage to articular cartilage is common, but the self-repair ability of cartilage is poor. If the damage continues to develop, it will cause dysfunction and seriously affect the quality of life. At present, autologous or allogeneic cartilage transplantation is commonly used in the clinical treatment of articular cartilage, but autologous cartilage transplantation causes great damage to patients, while allogeneic cartilage transplantation has limited sources and has potential problems of viral infection and immune rejection. The rapid development of tissue engineering methods provides new methods for the repair of cartilage defects, and scaffold materials play a vital ...

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