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Method for preparing double-layer bionic cartilage tissue engineering scaffold

A cartilage tissue and double-layer technology is applied in the field of preparing scaffolds for double-layer bionic cartilage tissue engineering, which can solve the problems of complex procedures and low bionic degree, and achieve the effects of good mechanical properties, simple operation and thin thickness.

Inactive Publication Date: 2013-03-06
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the method provided in this document requires the use of electrolysis equipment, and the process is relatively complicated, and its surface only relies on soaking to cover a layer of collagen, and the degree of biomimesis is low.

Method used

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  • Method for preparing double-layer bionic cartilage tissue engineering scaffold
  • Method for preparing double-layer bionic cartilage tissue engineering scaffold
  • Method for preparing double-layer bionic cartilage tissue engineering scaffold

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] This embodiment includes the following steps:

[0031] 1. Dissolve polylactic acid PLA in 1,4 epoxyhexane and stir for 24 hours to obtain a 7.4% polylactic acid PLA solution. Take the obtained solution and add it to a mold, and freeze it in a -20°C refrigerator for 4 hours to obtain a substrate;

[0032] Two, in 2% acetic acid aqueous solution, chitosan and collagen are added together in the ratio of 2% acetic acid aqueous solution by mass ratio 20: 1, the ratio of added chitosan and collagen mixture and 2% acetic acid aqueous solution is : 2.1g: 100ml, pour the obtained composite solution onto the surface of the substrate obtained in step 1, the pouring thickness is 2.5mm, pre-freeze at -20°C for 6h, then freeze-dry at -50°C and 80Pa for 48h, The substrate and the double-layer biomimetic cartilage scaffold with a thickness of 2.5 mm on the surface of the substrate are formed;

[0033] 3. After molding, soak the double-layer biomimetic cartilage scaffold with 5% NaOH s...

Embodiment 2

[0038] This embodiment includes the following steps:

[0039]1. Take pure 1,4 epoxy hexacycline and add it to the mold, put the mold into liquid nitrogen to freeze the 1,4 epoxy hexacyclic ring into a solid state for 10 minutes, put the frozen sample in a -20°C refrigerator for 1 hour , get the substrate;

[0040] Two, in 1.5% acetic acid aqueous solution, chitosan and collagen are added together in the ratio of 1.5% acetic acid aqueous solution by mass ratio 20: 1, the ratio of added chitosan and collagen mixture and 1.5% acetic acid aqueous solution is : 1.5g: 100ml, pour the obtained composite solution onto the surface of the substrate obtained in step 1, the pouring thickness is 2.5mm, pre-freeze at -20°C for 6h, then freeze-dry at -50°C and 80Pa for 48h, The substrate and the double-layer biomimetic cartilage scaffold with a thickness of 2.5 mm on the surface of the substrate are formed;

[0041] 3. After forming, the double-layer biomimetic cartilage scaffold on the su...

Embodiment 3

[0044] This embodiment includes the following steps:

[0045] 1. Dissolve polylactic acid PLA in 1,4 epoxyhexane and stir for 48 hours to obtain a 6.4% polylactic acid PLA solution. Add bone powder equivalent to polylactic acid PLA to the polylactic acid PLA solution and stir for 48 hours to mix evenly. Take The obtained solution was put into a mold, and placed in a refrigerator at -20°C for 4 hours to obtain a substrate;

[0046] 2. In 2% acetic acid aqueous solution, add chitosan or collagen, or add chitosan and collagen together in 2% acetic acid aqueous solution in any proportion, the added chitosan or collagen or chitosan and collagen The ratio of the mixture to 2% acetic acid aqueous solution is: 3.5g: 100ml, pour the resulting composite solution onto the surface of the substrate obtained in step 1, the pouring thickness is 3mm, pre-freeze at -20°C for 4h, and then Freeze-dry at -50℃, 90Pa for 48 hours, and form the substrate and the double-layer biomimetic cartilage sc...

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Abstract

The invention discloses a method for preparing a double-layer bionic cartilage tissue engineering scaffold, which comprises the following steps of: firstly, preparing a substrate; secondly, preparing the double-layer bionic cartilage tissue engineering scaffold on the surface of the substrate; and finally, soaking the double-layer bionic cartilage scaffold on the surface of the substrate by using solution of NaOH, and peeling the double-layer bionic cartilage scaffold from the substrate. The method has simple operation and high stability; the prepared double-layer bionic cartilage scaffold has a thin thickness and good mechanical properties and is favorable for clinical application; the scaffold is molded integrally; a vertical hole is tightly connected with a round hole to avoid the problem of breaking away from each other; the structure of the scaffold is similar to a natural cartilage structure and is hopeful to better maintain the phenotype of cartilage cells and improve the repairing effect on cartilage injuries; and the cartilage scaffold can be directly combined with a substrate comprising polylactic acid (PLA) / bone powder to form a bone cartilage scaffold used for repairing bone cartilage simultaneous colobomata, and a cartilage layer part still has a double-layer structure.

Description

technical field [0001] The invention relates to the technical field of scaffold preparation for cartilage tissue engineering, in particular to a preparation method for a double-layer bionic cartilage tissue engineering scaffold. Background technique [0002] Due to its special position and function in the body, damage to articular cartilage is common, but the self-repair ability of cartilage is poor. If the damage continues to develop, it will cause dysfunction and seriously affect the quality of life. At present, autologous or allogeneic cartilage transplantation is commonly used in the clinical treatment of articular cartilage, but autologous cartilage transplantation causes great damage to patients, while allogeneic cartilage transplantation has limited sources and has potential problems of viral infection and immune rejection. The rapid development of tissue engineering methods provides new methods for the repair of cartilage defects, and scaffold materials play a vital ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/40A61L27/56
Inventor 孙晓丹单程
Owner TSINGHUA UNIV
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