Production method of calcified layer-containing bionic tissue engineering bone cartilage integrated support

Abstract

The invention discloses a production method of a calcified layer-containing bionic tissue engineering bone cartilage integrated support. The method comprises the following steps: carrying out vacuum hot pressing on a produced transparent cartilage layer, a calcified cartilage layer and a subchondral bone layer, and carrying out particle leaching to produce the integrated composite material support with the middle being the dense calcified cartilage layer and with two sides being the porous transparent cartilage layer and the subchondral bone layer respectively, wherein the transparent cartilage layer and the subchondral bone layer are obtained through a solvent casting technology with polylactic acid as a matrix, calcium polyphosphate short fibers as a reinforcement and sodium chloride as a pore forming agent; and the close calcified cartilage layer (CPPf / PLLA film) is produced through a film layer spreading technology with polylactic acid as a matrix and calcium polyphosphate long fibers as a reinforcement. The content of CPP fibers is controlled to increase the strength, the rigidity and the osteoinductivity of the CPPf / PLLA integrated porous support and control the degradation rate of the support in a simulation body fluid.

Description

technical field [0001] The invention relates to a preparation method of an engineering osteochondral integrated support, belonging to the field of medical materials. Background technique [0002] Osteochondral defects in joints caused by trauma or bone disease are common clinically, mainly manifested as intractable pain and limited joint movement function, which seriously affect the quality of life of patients and have become one of the main causes of physical disability. The incidence rate in the United States is 1.5‰~3‰, and in my country it is about 5 to 6 times that of the United States, and it is increasing year by year with the increase of the proportion of middle-aged and elderly people. [0003] Since cartilage has no nerves, blood vessels, and lymphatic system, its self-repair ability is very limited, and cartilage defects with a diameter > 2 mm can hardly be completely repaired. If combined with subchondral bone defects, the treatment is even more difficult. Th...

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Problems solved by technology

For example, Gao et al. induced the expanded and cultured MSCs to osteoblast and chondrogenic phenotypes respectively, and then combined with chondrogenic scaffold HYAFF-II and osteogenic scaffold calcium phosphate to construct corresponding tissues, and then used fibrin gel to combine the two. Adhesion; in vitro experiments show that the combination of the two can form an osteochondral complex. Animal experiments show that the subchondral bone is well integrated with the host bone, but the cartilage integration is not good, and there is no tide line and calcification layer structure formation; (2) Integrated construction in vitro : First prepare the integrated scaffold material, then inoculate cartilage seed cells in the cartilage area, and inoculate or not inoculate osteogenic seed cells in the osteogenic area according to the situation; for example, Chen et al. use collagen sponge to make cartilage scaffolds, polylactide-polyethylene Lactide copolymer (PLGA) and collagen were used to make bone scaffolds, and dog MSCs were inoculated on the scaffolds, cultured in vitro for one week, and implanted in vivo to repair the osteochondral defect of the dog knee joint. Histological examination at 4 months after operation showed that cartilage was formed in the repaired area and bone, but poorer mechanical properties

(3) Integrated construction in vivo: the in vitro expanded and cultured seed cells are mixed with injectable biomaterials, and directly injected into the defect area to produce repair behavior. For example, Hoemann et al. mixed in vitro expanded chondrocytes with chitosan and passed arthroscopic Injected into rabbits to repair osteochondral defects, the implant was basically integrated with the surrounding tissue at 1 week after operation, and cartilage-like extracellular matrix was produced in histological observation, but the mechanical properties of the repaired tissue were poor