2results about How to "Prolong degradation time" patented technology

The application discloses a bovine type I alpha interferon-ferritin fusion protein, a mutant thereof, and a preparation method and application thereof. The bovine type I alpha interferon is fused with a ferritin subunit, the self-assembly characteristics of the ferritin are utilized, the interferon molecules are highly repeated and orderly displayed on the surface of the ferritin nanocage, and the expression level, structural stability and antiviral activity of the interferon are significantly improved. The fusion protein is further subjected to rational design mutation at a unit point or multiple points, and a mutant with significantly improved antiviral activity and stability is obtained. The fusion protein or the mutant thereof is expressed by using a silkworm or insect cell eukaryotic expression system, the expression system is safe in operation, simple in procedure, low in cost, and extremely beneficial to large-scale industrial production, and the prepared fusion protein or mutant nanometer particle has application prospects in the preparation of medicines or reagents for preventing or treating bovine viral diseases.

The application discloses injectable long-acting collagen and a preparation method and application thereof, and belongs to the technical field of collagen preparation. The application solves the problems of rapid degradation and insufficient mechanical property of collagen after injection into the body, and the problem of inability to have biological safety, good biological activity and injectability. The long-acting collagen is prepared by taking methoxypolyethylene glycol active ester and collagen as raw materials. Amide reaction is caused between the methoxypolyethylene glycol active ester and lysine residues on the collagen, hydrophilic PEG is introduced on the surface of the collagen, a hydration layer is formed, biological enzymes are hindered from contacting the collagen, collagen degradation is delayed, and the single-arm structure of the methoxypolyethylene glycol active ester can avoid product agglomeration and abnormal viscosity caused by excessive cross-linking. The long-acting collagen has a long retention time in the body, has no obvious cytotoxicity, has high biological safety, and maintains cell proliferation, migration and adhesion activity and biological repair functions.