Nanofiber bone cartilage repairing stent for tissue engineering and preparation method thereof

Abstract

The invention discloses a nanofiber bone cartilage repairing stent for tissue engineering and a preparation method thereof. The nanofiber bone cartilage repairing stent for tissue engineering is characterized by comprising a porous cartilage layer, a porous cartilage lower bone layer and a connecting layer between the porous cartilage layer and the porous cartilage lower bone layer, wherein the porous cartilage layer includes a PLLA-based composite degradable polymer material and is of a porous nanofiber structure which is 20-400mu m in pore diameter; the connecting layer includes a PLLA-based composite degradable polymer material and is of a porous nanofiber structure which is less than 5mu m in pore diameter; and the porous cartilage lower bone layer comprises a PLLA-based composite degradable polymer material containing 5-70% (w / w) of nano-hydroxyapatite and is of a porous nanofiber structure which is 50-500mu m in pore diameter. Microstructure of the bone cartilage repairing stent prepared by the invention is represented as bionic ECM nanofiber; the repairing stent can promote adhesion, propagation and differentiation of related bone cartilage cells on the stent, and can also promote transportation of nutrient substances in the stent and discharge of metabolic waste.

Description

technical field [0001] The invention belongs to the technical field of osteochondral tissue engineering scaffolds, in particular to a tissue engineering nanofiber osteochondral repair scaffold and a preparation method thereof. Background technique [0002] The damage and lesions of articular cartilage are common orthopaedic diseases in clinic, and it is difficult to self-repair. Moreover, the damage of the cartilage at the joint is often accompanied by the joint damage of the subchondral bone layer, so the repair of the cartilage also needs to repair the subchondral bone. At present, the commonly used clinical treatment method is autologous or allogeneic osteochondral transplantation, which has achieved a certain therapeutic effect, but there are also shortcomings. The source of autologous osteochondral is limited, and it will cause secondary damage to patients. risk of disease transmission. The development of tissue engineering provides a new way to construct osteochondra...

AI Technical Summary

Problems solved by technology

Chinese patent (CN 102872480A) discloses a biphasic osteochondral tissue engineering scaffold, the cartilage layer and subchondral bone layer are both porous structures, but microscopically it is not the nanofibrous structure of bionic ECM

Moreover, since the two layers are connected porous structures, it is easy for the related cells of the cartilage layer and the subchondral bone layer to migrate and grow into each other, which affects the repair of the functions of the cartilage layer and the subchondral bone layer.

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