Double-gene activated bone-cartilage compound transplant, preparation method and application thereof

A dual-gene, graft technology, applied in the direction of prosthesis, medical science, etc., can solve the problems of poor stability of the graft and poor integration of the host tissue

Inactive Publication Date: 2010-06-09
NANJING UNIV
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

In addition, there is a major problem in pure cartilage repair: whether natural cartilage or tissue engineered cartilage is transplanted, the stability of the graft and the integration with the host tissue are relatively poor

Method used

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  • Double-gene activated bone-cartilage compound transplant, preparation method and application thereof
  • Double-gene activated bone-cartilage compound transplant, preparation method and application thereof
  • Double-gene activated bone-cartilage compound transplant, preparation method and application thereof

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Embodiment Construction

[0028] The invention specifically relates to planting seed cells on two double-layer scaffold materials with the same main components activated by different genes, and using the principle of in situ gene transfection to simultaneously induce the upper and lower layers of seed cells to chondrocytes and osteoblasts respectively. Differentiation of cells to form tissue-engineered bone-cartilage composite grafts in vitro. In the specific implementation process, it mainly involves the production of chitosan-gelatin scaffolds, the construction of gene-activated chitosan-gelatin Inoculation and culture of mesenchymal stem cells and construction of dual-gene activated double-layer scaffolds.

[0029] 1. Fabrication of Chitosan-Gelatin Scaffolds

[0030] ①Add 1g of chitosan and 1g of type B gelatin to the preheated 50mL 1% acetic acid solution.

[0031] ②Stir at 60°C for 12 hours to prepare a chitosan-gelatin mixed solution with a concentration of 2%.

[0032] ③ After pouring 1mL chi...

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Abstract

The invention belongs to the technical field of tissue engineering and gene delivery, in particular to a double-gene activated bone-cartilage compound transplant, a preparation method and application thereof. In the method, seed cells are respectively planted on two layers of bracket materials which are activated by different genes and have the same main constituents, and the seed cells of the upper layer and the lower layer are simultaneously induced to respectively differentiate to chondrocyte and osteoblast by utilizing an in situ gene transfection principle so as to form a tissue engineering bone-cartilage compound transplant in vitro, wherein the seed cells are bone mesenchymal stem cells; the main constituents of the bracket materials are chitosan and gelatin; and in the two different genes, one is a gene differentiated from any known induced stem cell to the chondrocyte, while the other one is a gene differentiated from any known induced stem cell to the osteoblast. By utilizing the method, the bone-cartilage compound transplant is prepared in vitro and further used for restoring bone-cartilage.

Description

1. Technical field [0001] The invention belongs to the technical field of tissue engineering and gene delivery, and in particular relates to a preparation method and application of a double-gene activated bone-cartilage composite graft. 2. Background technology [0002] Applying tissue engineering methods to osteogenesis and cartilage repair is one of the hotspots in plastic medicine research in recent years. Compared with traditional methods such as arthroplasty, microfracture, autologous osteoblasts or chondrocyte transplantation, tissue engineering methods can obtain osteogenic and cartilage tissues with more biological and mechanical functions, and are easy to operate and controllable. Strong, it can be transplanted to the defect site in vivo after obtaining sufficient cells and extracellular matrix in vitro. [0003] The introduction of gene therapy into the construction of tissue-engineered bone and cartilage has attracted more and more attention from researchers in t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/40A61L27/38A61L27/22A61L27/20A61L27/54
Inventor 张峻峰陈江宁刁华佳陈欢李沛
Owner NANJING UNIV
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