Collagen basal bone cartilage three-layer compound and preparation method thereof

A composite, osteochondral technology, applied in medical science, prosthesis, etc., to prevent splitting between layers and improve interface bonding performance

Active Publication Date: 2012-12-05
ZHEJIANG XINGYUE BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a method for constructing an integrated three-layer collagen-based osteochondral biomaterial, that is, a three-layer collagen-based osteochondral composite, which overcomes the defects of physical and chemical connection methods used in the construction of traditional osteochondral multilayer structures

Method used

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  • Collagen basal bone cartilage three-layer compound and preparation method thereof
  • Collagen basal bone cartilage three-layer compound and preparation method thereof
  • Collagen basal bone cartilage three-layer compound and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0029] Example 1 Extraction of Water-Soluble Type I Collagen

[0030] ①Take off the tendons from the fresh pork legs of two-month-old healthy white pigs, remove the fascia and fat, cut them into 120g tendon slices, soak them in 500mL of ethanol solution with a volume concentration of 75%, and overnight at 4°C; ②The tendons from step ① Rinse 95g slices with distilled water 5 times, add 0.5mol / L acetic acid aqueous solution 1800mL, adjust pH to 1 with 6M sodium hydroxide aqueous solution, add pepsin (Sigma-ALDRICH, P7000-25G, 1:10000, 601U / mg) 9g, placed at 4°C for 3 days to obtain 1800mL collagen sol solution; ③Centrifuge the collagen sol solution obtained in step ② at 4°C and 4000rpm for 25 minutes, discard the upper fat layer, and dilute the precipitate a with 0.5mol / L acetic acid aqueous solution for 3 double the volume, filter it with 4 layers of gauze in an ultra-clean bench, mix the filtrate with 0.9 mol / L NaCl aqueous solution at a volume ratio of 1:10, shake intermitten...

Embodiment 2

[0032] (1) Dissolve the water-soluble type I collagen extracted by the method in Example 1 in deionized water to prepare 10 mL of 10 mg / mL collagen aqueous solution, 5 mL of 10 mg / mL collagen aqueous solution, and 5 mL of 35 mg / mL collagen aqueous solution; Dissolve dipotassium hydrogen phosphate in 10 mL of the above-mentioned 10 mg / mL collagen aqueous solution (collagen quality is 100 mg) to prepare a collagen-phosphate mixed solution; take ten polytetrafluoroethylene cylindrical molds with a depth of 8 mm and a diameter of 8 mm, each The molds were first injected with a 5mm height of collagen-phosphate mixed solution, frozen at -20°C for 24 hours, and then added with a 2mm height of 10mg / mL collagen aqueous solution, after standing at 0°C for 1min, then frozen at -20°C for 24 hours After that, inject 35mg / mL collagen aqueous solution at a height of 1mm, let stand at 0°C for 1min, freeze at -20°C for 24 hours, remove the mold, and obtain dense collagen-porous collagen-collage...

Embodiment 3

[0036] (1) Dissolve the water-soluble type I collagen extracted by the method in Example 1 in deionized water to prepare 10 mL of 20 mg / mL collagen aqueous solution, 5 mL of 15 mg / mL collagen aqueous solution, and 5 mL of 40 mg / mL collagen aqueous solution; Dipotassium hydrogen dipotassium was dissolved in 10 mL of the above 20 mg / mL collagen aqueous solution to prepare a collagen-phosphate mixed solution; take ten polytetrafluoroethylene cylindrical molds with a depth of 8 mm and a diameter of 8 mm, and each mold was first injected with 4 mm high Collagen-phosphate mixed solution, freeze at -50°C for 16 hours, then inject 15mg / mL collagen aqueous solution at a height of 3mm, let stand at 4°C for 5min, freeze at -50°C for 16 hours, and then inject at a height of 1mm 40mg / mL collagen aqueous solution, after standing at 4°C for 5min, freeze at -50°C for 16 hours, remove the mold, and obtain dense collagen-porous collagen-collagen water-soluble phosphate complex;

[0037] (2) Dis...

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PUM

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Abstract

The invention discloses a collagen basal bone cartilage three-layer compound and a preparation method of the compound. The compound is prepared with the following method: sequentially injecting collagen-phosphate mixed solution, 5-20mg / mL of collagen water solution and 35-45mg / mL of collagen water solution into a mold; forming a dense collagen - multiple orifice collagen - collagen water soluble phosphoric acid compound; and after the compound is immerged by absolute ethyl alcohol, and absolute ethyl alcohol of calcium salt, crosslinking the compound by using the MES solution of EDC-NHS to obtain the collagen basal bone cartilage three-layer compound. The preparation method disclosed by the invention improves the interface bonding property of a three-layer bracket, and prevents the unfavorable crack consequence between layers occurred during the cartilage renovation process.

Description

(1) Technical field [0001] The invention relates to the field of biomaterial preparation, in particular to a collagen-based osteochondral three-layer composite for osteochondral repair using collagen as a matrix material to construct an integrated structure and a preparation method. (2) Background technology [0002] Osteochondral injuries caused by traffic accidents, sports, and various arthritis diseases have brought great pain and heavy economic burden to patients and their families. It affects the quality of life of patients and increases social pressure. At present, autologous osteochondral transplantation and allogeneic bone or xenogeneic osteochondral transplantation are generally used in the clinical treatment of articular osteochondrosis. The former has limited sources and creates new points of damage, while the latter presents a risk of disease. Therefore, they cannot be widely used. [0003] The proposal of tissue engineering has gradually applied biomaterials ...

Claims

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Application Information

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IPC IPC(8): A61L27/40C08J9/40C08J9/28C08J3/24
Inventor 赵洪石陈隆坤其他发明人请求不公开姓名
Owner ZHEJIANG XINGYUE BIOTECH
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