A kind of articular cartilage vitrification cryoprotectant solution and cartilage preservation method

Abstract

The invention discloses a vitrified cryoprotective solution for articular cartilage and a cartilage preservation method. Each 1000ml vitrified cryoprotectant solution contains: 99.9% dimethyl sulfoxide 150-250ml, acetamide 110-200g, 1,2-propylene glycol 70 ~120ml, polyethylene glycol 10~80g, trehalose 20~100g, the rest is deionized water. Soak the osteochondral pieces in gradient vitrification protection solution at 4°C for 10 minutes, seal them in refrigerated tubes, and put them in liquid nitrogen for storage; take them out before transplantation for rewarming, rewarm in a water bath at 37°C for 10 seconds, and then Put them into 10% and 5% sucrose solutions at 37°C successively and stay for 5 minutes each, and finally rinse with phosphate buffer solution for 3 times before transplantation. The invention enables long-term preservation of articular cartilage tissue, maintains a relatively high survival rate of cartilage cells, improves the preservation effect of osteochondral grafts, and is used for cartilage tissue bank and clinical cartilage transplantation.

Description

technical field

[0001] The invention belongs to the technical field of biomedicine, and in particular relates to a vitrification cryoprotectant solution for articular cartilage and a cartilage preservation method. Background technique

[0002] In recent years, with the improvement of people's living standards in our country and the comprehensive popularization of sports, the number of patients with articular cartilage injuries has increased significantly. A series of encouraging progress has been made in the repair of articular cartilage defects by allogeneic osteochondral transplantation. Cartilage transplantation is one of the very promising repair methods for the treatment of cartilage lesions. Because the allogeneic cartilage graft material is relatively easy to obtain and can be prefabricated into any shape and size, it is convenient to make the normal functional articular cartilage into various shapes and implant it in the bone defect to restore the shape of the carti...

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