Cultivation in vitro method for artificial cartilage or bone cartilage with different curve and bioreactor thereof

A bioreactor and in vitro culture technology, applied in the field of bioreactors, can solve problems that do not involve rolling loading, etc.

Inactive Publication Date: 2008-12-03
TIANJIN UNIVERSITY OF TECHNOLOGY
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  • Application Information

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Problems solved by technology

But none of them involve the couplin

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  • Cultivation in vitro method for artificial cartilage or bone cartilage with different curve and bioreactor thereof
  • Cultivation in vitro method for artificial cartilage or bone cartilage with different curve and bioreactor thereof
  • Cultivation in vitro method for artificial cartilage or bone cartilage with different curve and bioreactor thereof

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example

[0031] Culture method for cartilage defect

[0032] A piece of cartilage was biopsied from the non-weight-bearing area of ​​the patient, fully rinsed with D-Hanks solution, and then initially chopped into 1-2 mm3, then transferred into a penicillin vial, digested with trypsin and collagenase, centrifuged to obtain chondrocytes, transferred into a culture bottle and added The culture medium is cultivated, and the cells are used after expansion.

[0033] The expanded chondrocytes were harvested by digestion. Cells were resuspended in fibrinogen solution to 2×10 6 / ml, mix through a Y-shaped co-push needle, pour into the mold, and stand at 37°C for 10 minutes to fully solidify to form a rectangular plate-shaped composite. Then move it to the culture room, fix it to the bottom plate of the culture room, and add culture medium to cultivate.

[0034] The reactor is placed in an incubator with O 2 , CO 2 , The temperature is automatically controlled, and the peristaltic pump dri...

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Abstract

The invention discloses a method for culturing an artificial cartilage with different curved surfaces or a bone cartilage under the action of rolling load and sliding load. The method comprises an incubator which contains O2 and CO2 and can perform temperature adjustment and cell culture, perfusion and other conditions which are used for keeping culture solution fresh, and active factors which are added to the culture solution. The method is characterized in that culture blanks with different curved surfaces are fixed on the bottom plate of a culture room; during the culturing process, a culture is subjected to the effect of the rolling load and the sliding load simultaneously, and the culture is cultured into cartilage tissues or bone cartilage complex tissues. The method has the advantages that under the effect of the rolling load and the sliding load, the loading way of the method is closer to the mechanical environment of the growth and the development of the cartilage tissues; and under the loading condition, the method is favor of the growth and the development of cells and tissues. An experiment shows that mucopolysaccharide secreted by cartilage cells under the effect of the rolling load and the sliding load is higher than the mucopolysaccharide secreted by the cartilage cells only under the effect of the rolling load. After being cultured, the cultures with different shapes can be taken as implants, and the method of the invention is applied in repairing the defect of the cartilage in different parts.

Description

【Technical field】 [0001] The invention relates to the field of tissue engineering, in particular to a method for cultivating artificial cartilage or osteocartilage of different shapes and a related bioreactor under the action of rolling load and sliding load. 【Background technique】 [0002] Tissue engineering is a new discipline emerging in recent years. It is another new milestone in the history of life science development after cell biology and molecular biology. new era. Tissue engineering will become a high-tech industry with great potential in this century, and it will surely produce huge social and economic benefits. [0003] Articular cartilage defect is one of the common diseases in surgery, usually caused by trauma, surgical resection, osteoarthritis and other chronic diseases. The clinical manifestations are intractable pain, joint movement disorders, and severe cases can completely lose joint function, becoming one of the main reasons for limb disability and lab...

Claims

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Application Information

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IPC IPC(8): C12N5/08C12M3/00A61L27/38A61F2/30C12N5/077
CPCA61F2/30756A61F2002/30001
Inventor 张春秋王月新崔元璐刘海英
Owner TIANJIN UNIVERSITY OF TECHNOLOGY
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