72results about How to "Improve tissue repair ability" patented technology

The invention relates to a biological activity mineral substance material and application of biological activity mineral substance material to soft tissue anabrosis and long-time erosion wound cell regeneration and melanoma restraining. The application is mainly achieved by wrapping biological activity mineral substance material powder with the particle size smaller than 10 micrometers by medical glycerinum into gel and coating the surfaces of wounds with the gel. The biological activity mineral substance material is prepared from, by mass, 36% of SiO2, 5% of Na2O, 20% of CaO, 15% of P2O5, 5% of medical carboxymehyl chitosan, 5% of medical sodium hyaluronate, 10% of PEG, 2% of allantoin and 2% of tocopheryl acetate. The biological activity mineral substance material is particularly suitable for treating soft tissue anabrosis such as diabetic ulcer, dental ulcer, cervical erosion, fat liquefaction wounds, bedsores, sinus tracts, venereal disease ulcer, hemorrhoids and wounds.

The invention discloses a cross-linked sodium hyaluronate biomembrane and a preparation method thereof. The cross-linked sodium hyaluronate biomembrane is prepared by: conducting freeze drying on a prepared gel with a crosslinking degree up to 60%-80%, and then pressing the freeze-dried gel into a membrane. The preparation method of the gel includes: acquiring cross-linked sodium hyaluronate dry powder, then washing the collected sieved powder by dimethyl sulfoxide (DMSO); washing the DMSO washed powder by ethanol; then conducting vacuum drying to obtain cross-linked sodium hyaluronate powder; fully swelling the powder, performing purification for 6-10h at a room temperature of 15-35DEG C, then carrying out homogenization micronization treatment in a high-speed dispersion machine, and then collecting the uniform gel particles, thus obtaining the cross-linked sodium hyaluronate gel used for biomembrane preparation. The cross-linked sodium hyaluronate biomembrane prepared by the method provided by the invention has is pyrogen-free, sterile, free of residual crosslinking agent, and has low cytotoxicity. With long residence time in vivo, the biomembrane has good anti-adhesion effect, good biocompatibility and tissue repair performance.

The invention relates to a degradable nano-short fiber material for tissue repair. The nano-short fiber material is composed of nano-short fibers, wherein the diameter of the nano-short fibers is 200-800 nm, and the length of the nano-short fibers does not exceed 500 [mu] m; the length of at least 93% of the nano-short fibers in the nano-short fiber material is within 20-200 [mu] m, and the stacking density of the nano-short fiber material is 0.001-0.099 g/cm<3>. According to the nano-short fiber material, the length of the nano-fibers is shortened, the dispersing performance and repairing performance of the nano-fibers are improved, and the application range of the nano-short fiber material is expanded.

The invention discloses a preparation method of collagen sponge. The method comprises the following steps: carrying out enzymolysis on cattle heel tendon in protease aqueous solution at pH value of 1.0-5.0; centrifuging the enzymolysis liquid, collecting supernatant, and salting out collagen with saturated salt solution; dialyzing salted-out collagen; cross-linking the dialyzed collagen in a cross-linking agent aqueous solution; and freeze-drying cross-linked collagen gel, cross-linking the collagen in a cross-linking agent aqueous solution and freeze-drying to obtain the collagen sponge. The collagen sponge obtained by the preparation method of the collagen sponge disclosed by the invention has certain elastic and tensile toughness after being immersed in water, and has good biocompatibility and tissue repairing performance, and high protein content and is controllable in degradation.

The invention relates to a composite film for guiding bone tissue regeneration and a preparation method of the composite film. The composite film is a double-layer film, wherein a bottom-layer film is an L-lactide and caprolactone copolymer (L-PLCA) cast film with a compact structure, and the molar ratio of monomer components is (50:50) to (95:05); a surface layer is an L-PLCA and hydroxyapatite (HA) or tricalcium phosphate (beta-TCP) compound electrospinning film with a micropore structure, wherein the mass ratio of HA or beta-TCP in the electrospinning film is 10-50wt%, and the particle size of the electrospinning film can be nano-grade or micro-grade. The double-layer composite film is relatively good in mechanical performance and bone-like apatite deposition induction capacity, favorable in flexibility and capable of realizing performance regulation through regulating the molar ratio of L-PLCA, the content of the component HA or beta-TCP and the microstructure of the electrospinning film so as to be a novel degradable double-layer composite film capable of guiding bone tissue regeneration.

The invention belongs to the technical field of medicine and hygiene, and in particular relates to a temperature-sensitive medical chitosan derivative preparation and a preparation method of the temperature-sensitive medical chitosan derivative preparation. The main ingredient, namely, chitosan derivative in the preparation is hydroxybutyl chitosan. The product has the temperature sensitivity, and has good effects of bleeding stopping, adhesion preventing, drug sustained release, and prevention and treatment for osteoarthritis. The preparation method of the temperature-sensitive medical chitosan derivative preparation has the advantages that large-scale production can be realized, high-pressure steam sterilization or filtration sterilization can be realized, and viruses, immunogenicity and the like can be removed.

The invention relates to the field of medicines and daily chemicals, in particular to a periplaneta americana new polypeptide for promoting tissue repair and application of the periplaneta americana new polypeptide, the structure of the periplaneta americana new polypeptide is shown as a formula I, and the periplaneta americana new polypeptide is named as a tissue repair factor PA1. The new polypeptide can promote proliferation of mouse embryo fibroblasts and migration of human immortalized epidermal cells even at a low concentration, has low toxic and side effects and has a good application prospect, so that the polypeptide can be used for preparing medicines for treating wounds, scalds and ulcers or cosmetics and daily chemical products for improving skin beauty.

The invention relates to injectable decellularized small intestinal submucosal matrix particles and a preparation method and the application thereof, and belongs to the field of regenerative medicine.The preparation method of the injectable decellularized small intestinal submucosal matrix particles comprises the following steps: (1), taking an animal small intestine tissue, and sequentially rinsing, sterilizing and repeatedly freezing and thawing; (2), shearing the animal small intestine tissue, scraping off a mucosal layer, a muscle layer and a serosa layer of the small intestine to obtaina stripped small intestine submucosa, and soaking the stripped small intestine submucosa in a sodium chloride solution; (3), decellularizing the small intestine submucosa, and then rinsing; (4), soaking the small intestine submucosa in a buffer solution, then putting into a descaling agent, extracting while shaking, and rinsing; (5), soaking the small intestine submucosa in the buffer solution, performing freeze drying, and grinding to obtain the injectable decellularized small intestinal submucosal matrix particles. The injectable decellularized small intestinal submucosal matrix particles prepared by the preparation method provided by the invention are rich in bioactive component and structurally loose and porous.

The invention discloses a method for preparing a bamboo nanofiber composite regenerated cellulose tissue repairing material and belongs to the technical field of tissue repairing material preparation. The method comprises the following steps: preparing a bamboo nanofiber solution, preparing a regenerated cellulose spinning solution, and performing electrostatic spinning, thereby obtaining the repairing material. The tissue repairing material prepared by using the method has good antibacterial properties, mechanical properties, tissue repairing properties and medicinal adhesion properties.

The present disclosure relates to a polycaprolactone microsphere filler containing collagen peptide and a preparation method therefor. Provided is a polycaprolactone microsphere filler obtained by encapsulating collagen peptide in polycaprolactone microspheres, which, when injected into a living body, exhibits a rapid collagen formation effect as well as a high tissue restoration property and maintains the effects for a long period of time, thereby showing excellent restoration or volume expansion or wrinkle improvement properties of soft tissues such as cheeks, breasts, nose, lips, and buttocks.

The invention relates to a preparation method of a silk fibroin compound bioglass tissue repair material and belongs to the field of biomedical materials. The preparation method comprises the following steps: preparing a silk fibroin nano solution; preparing a bioglass solution; and performing electrostatic spinning to prepare the repair material. The silk fibroin can improve the mechanical properties of the material and the bioglass can improve the medical property of the material. The tissue repair material prepared by the invention has good mechanical properties, tissue repair property and medical adhesiveness.

The invention relates to a modified tussah silk fibroin 3D printing support and a preparation method thereof. Core part printing ink prepared by using chemically modified tussah silk fibroin nano microfibers and shell part printing ink prepared by using a chemically modified tussah silk fibroin nano microfiber/gelatin composite system are used for carrying out 3D printing to prepare a modified tussah silk fibroin 3D printing support. The compression modulus of the modified tussah silk fibroin 3D printing support is 100 to 600 MPa after soaking in genipin with the mass concentration of 0.1 to 5wt% and subjection to a cross-linking reaction for 24 h; and after culture for 10 days, the survival rate and the proliferation rate of induced pluripotent stem cells are high. The finally prepared modified tussah silk fibroin 3D printing support comprises printing lines with core-shell structures. According to the invention, the preparation method is relatively simple; and the prepared 3D printing support has excellent mechanical properties, excellent biocompatibility and good tissue repair capability.

The invention provides a preparation method of a cod skin collagen peptide composite regeneration fiber restoration material, and belongs to the technical field of a tissue restoration material. The method comprises the steps of preparing cod skin collagen peptide, preparing bamboo nanometer fiber solution, preparing regenerated cellulose spinning liquid, performing static spinning, and obtaining the restoration material. The prepared tissue restoration material has good antibacterial performance, mechanical performance, tissue restoration performance and medicine addition performance.

The invention provides regenerated cellulose wound dressing and a preparation method thereof, and belongs to the field of composite biological materials. The wound dressing comprises, by weight, 60-70 parts of cotton linter, 5-10 parts of chitosan solution, 5-10 parts of alginate and 5-10 parts of silk protein solution. The prepared bombax ceiba composite regenerated cellulose wound dressing can be used for repairing and healing of various wound surfaces, and has a good tissue repair capacity.

The invention provides mesenchymal stem cells for treating enteritis and a preparation method of the mesenchymal stem cells. In the process of culturing the mesenchymal stem cells, epithelial cell growth factors, vascular endothelial cell growth factors and prostaglandin E2 are added, so that the tissue repair capacity of the mesenchymal stem cells is improved; and in the process of culturing themesenchymal stem cells, fibroblast growth factors, platelet-derived growth factors B and prostaglandin E2 are added to improve the immunoregulation capacity of the mesenchymal stem cells. According tothe mesenchymal stem cells for treating enteritis, the tissue repair capability of the mesenchymal stem cells can be effectively enhanced by adding the cytokines in stages, and the immunoregulation capability of the mesenchymal stem cells can be effectively improved. The mesenchymal stem cells induced by the combined factors are used for treating enteritis, and the method is simple and economical, is easy for industrial operation, and is easy for clinical transformation and application.

The invention provides a scaffold material capable of recruiting endogenous mesenchymal stem cells. The scaffold material is prepared by subjecting a freeze-dried sponge scaffold formed by freeze-drying hydrogel which is prepared by crosslinking sulfhydrylated hyaluronic acid and dopamine modified silk fibroin and has a composite three-dimensional network structure to soaking treatment with a maleimide polyethylene glycol active ester solution and an E7 peptide solution with one end connected with cysteine in sequence, and then grafting E7 peptide on the freeze-dried sponge scaffold through maleimide polyethylene glycol active ester and carrying out drying so as to form an inter-communicating three-dimensional porous structure. The invention also provides a preparation method of the scaffold material and application of the scaffold material as a cartilage tissue engineering three-dimensional scaffold or a cartilage tissue engineering three-dimensional cell scaffold. The scaffold material provided by the invention can provide an appropriate microenvironment and appropriate mechanical properties for the growth of cells, and has the performance of recruiting endogenous mesenchymal stem cells.

The invention provides a preparation method for a sheep placenta-regenerated fiber composite repair material, belonging to the technical field of tissue repair materials. The preparation method comprises the following steps: preparation of sheep placenta extract; preparation of a milk protein solution; preparation of a regenerated-cellulose spinning solution; and electrostatic spinning for preparation of the repair material. The repair material prepared in the invention has good antibacterial properties, mechanical properties, tissue repairing performance and drug adhesion.

The invention discloses a porous titanium surface collagen coating and a preparation method thereof. The method provided by the invention adopts electrostatic spraying technique to prepare a collagencoating on a porous titanium alloy surface, conducts bioactivation modification on the titanium alloy surface, and improves the biocompatibility and tissue repair ability of the titanium alloy material. The collagen coating clings to a porous titanium alloy skeleton in a bead form, also can coexist with a porous titanium alloy surface micro-arc oxidation structure, and realizes complementation ofthe advantages of the two technologies. At the same time, the biological evaluation test result indicates that the collagen coating prepared by the method provided by the invention can meet the requirements of implantable medical devices.

The invention relates to a preparation method of a periostracum cicada compound bioglass tissue repair material, belonging to the field of biological medicine materials. The preparation method comprises the steps of preparing a periostracum cicada extracting solution, preparing a silk fibroin nano-solution, preparing a bioglass solution, and carrying out electrostatic spinning, so as to obtain the repair material, wherein periostracum cicada has certain sterilization and antiphlogosis effects, silk fibroin is capable of changing the mechanical properties of the material, and bioglass is capable of changing the medical properties of the material. The tissue repair material prepared by virtue of the preparation method has good mechanical properties, tissue repair performance and drug adhesiveness.