Mesenchymal stem cells for treating enteritis and preparation method of mesenchymal stem cells

A technology for mesenchymal stem cells and enteritis, which is applied in the field of mesenchymal stem cells for the treatment of enteritis and their preparation, and can solve the problems of ineffective anti-inflammatory and regulating immune function of the body and the like

Pending Publication Date: 2021-03-19
山东佰傲干细胞生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, mesenchymal stem cells have certain effects in the treatment of colitis, but in the treatment of severe colitis, they have little effect in anti-inflammation and regulating the immune function of the body

Method used

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  • Mesenchymal stem cells for treating enteritis and preparation method of mesenchymal stem cells
  • Mesenchymal stem cells for treating enteritis and preparation method of mesenchymal stem cells
  • Mesenchymal stem cells for treating enteritis and preparation method of mesenchymal stem cells

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Experimental program
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Embodiment 1

[0033] This embodiment provides a kind of mesenchymal stem cells for treating enteritis, said mesenchymal stem cells are mesenchymal stem cells induced and cultured by combination factors, said combination factors include fibroblast growth factor, platelet-derived growth factor B , epithelial growth factor or vascular endothelial growth factor.

[0034] In some embodiments, the concentration of fibroblast growth factor is 1 ng / mL-30 ng / mL, such as 1 ng / mL, 2 ng / mL, 10 ng / mL, 15 ng / mL, 30 ng / mL. The concentration of platelet-derived growth factor B is 1 ng / mL-30 ng / mL, for example, 1 ng / mL, 2 ng / mL, 10 ng / mL, 15 ng / mL, 30 ng / mL.

[0035] In some embodiments, the concentration of the epithelial growth factor is 1 ng / mL-50 ng / mL, such as 1 ng / mL, 2 ng / mL, 10 ng / mL, 15 ng / mL, 30 ng / mL. The concentration of vascular endothelial growth factor is 1ng / mL-50ng / mL, such as 1ng / mL, 2ng / mL, 10ng / mL, 15ng / mL, 30ng / mL, 45ng / mL, 50ng / mL.

Embodiment 2

[0037] This embodiment provides a kind of mesenchymal stem cells for treating enteritis, said mesenchymal stem cells are mesenchymal stem cells induced and cultured by combination factors, said combination factors include fibroblast growth factor, platelet-derived growth factor B , epithelial growth factor, vascular endothelial growth factor, prostaglandin E or prostaglandin E2.

[0038] In some embodiments, the concentration of fibroblast growth factor is 1 ng / mL-30 ng / mL, such as 1 ng / mL, 2 ng / mL, 10 ng / mL, 15 ng / mL, 30 ng / mL. The concentration of platelet-derived growth factor B is 1 ng / mL-30 ng / mL, for example, 1 ng / mL, 2 ng / mL, 10 ng / mL, 15 ng / mL, 30 ng / mL.

[0039] In some embodiments, the concentration of the epithelial growth factor is 1 ng / mL-50 ng / mL, such as 1 ng / mL, 2 ng / mL, 10 ng / mL, 15 ng / mL, 30 ng / mL. The concentration of vascular endothelial growth factor is 1ng / mL-50ng / mL, such as 1ng / mL, 2ng / mL, 10ng / mL, 15ng / mL, 30ng / mL, 45ng / mL, 50ng / mL.

[0040] In some ...

Embodiment 3

[0042] This embodiment provides a mesenchymal stem cell preparation for treating enteritis, the mesenchymal stem cell preparation includes mesenchymal stem cells and auxiliary components, and the auxiliary components include a volume fraction of 94.5% physiological saline with a volume fraction of 5% % glucose injection, low molecular weight heparin calcium injection with a volume fraction of 0.5%.

[0043] The mesenchymal stem cells are mesenchymal stem cells induced and cultured by a combination of factors including fibroblast growth factor, platelet-derived growth factor B, epithelial growth factor, vascular endothelial growth factor, prostaglandin E or Prostaglandin E2.

[0044]In some embodiments, the concentration of fibroblast growth factor is 1 ng / mL-30 ng / mL, such as 1 ng / mL, 3 ng / mL, 9 ng / mL, 12 ng / mL, 30 ng / mL. The concentration of platelet-derived growth factor B is 1 ng / mL-30 ng / mL, for example, 1 ng / mL, 4 ng / mL, 8 ng / mL, 18 ng / mL, 30 ng / mL.

[0045] In some emb...

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Abstract

The invention provides mesenchymal stem cells for treating enteritis and a preparation method of the mesenchymal stem cells. In the process of culturing the mesenchymal stem cells, epithelial cell growth factors, vascular endothelial cell growth factors and prostaglandin E2 are added, so that the tissue repair capacity of the mesenchymal stem cells is improved; and in the process of culturing themesenchymal stem cells, fibroblast growth factors, platelet-derived growth factors B and prostaglandin E2 are added to improve the immunoregulation capacity of the mesenchymal stem cells. According tothe mesenchymal stem cells for treating enteritis, the tissue repair capability of the mesenchymal stem cells can be effectively enhanced by adding the cytokines in stages, and the immunoregulation capability of the mesenchymal stem cells can be effectively improved. The mesenchymal stem cells induced by the combined factors are used for treating enteritis, and the method is simple and economical, is easy for industrial operation, and is easy for clinical transformation and application.

Description

technical field [0001] The invention belongs to the field of chondrocyte culture, and in particular relates to a mesenchymal stem cell for treating enteritis and a preparation method thereof. Background technique [0002] Mesenchymal stem cells are a type of stem cells with self-renewal, proliferation and multi-directional differentiation potentials, widely present in umbilical cord, bone marrow, dental pulp, adipose or placental tissue, with the advantages of easy collection, storage and transportation, no allogeneic rejection, avoiding ethical Controversy, stimulate tissue regeneration, regulate immune function and many other advantages. [0003] The therapeutic drugs for colitis are mainly concentrated in traditional Chinese medicine and probiotic drugs. The main function of these drugs is to regulate the gastrointestinal flora and inhibit inflammation, but they have little effect on the treatment of severe inflammation, and they have not fundamentally improved the body's...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/28A61P29/00A61P1/00C12N5/0775
CPCA61K35/28A61P29/00A61P1/00C12N5/0662C12N2501/113C12N2501/115C12N2501/119C12N2501/135C12N2501/02
Inventor 程蕊苹周凤胡成虎
Owner 山东佰傲干细胞生物技术有限公司
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