Composite film for guiding bone tissue regeneration and preparation method of composite film

A bone tissue regeneration and composite technology, applied in medical science, surgery, etc., can solve the problem of unsatisfactory mechanical properties and degradation properties of PCL, unfavorable deposition of bone-like apatite, cell adhesion, and further improvement in degradation properties, etc. problems, to achieve the effect of being conducive to surgical operation, good physical barrier effect, and long-term space maintenance ability

Inactive Publication Date: 2014-09-03
CHENGDU ORGANIC CHEM CO LTD CHINESE ACAD OF SCI
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  • Abstract
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Problems solved by technology

[0004] In the film-forming method, the composite film with high mechanical strength can be obtained by directly blending the inorganic particles and the polymer solution, and then the casting method can form a film, which has a good mechanical barrier effect, but it lacks flexibility and poor adhesion. At the same time, the dense structure of the membrane surface is not conducive to the deposition of bone-like apatite and the adhesion of cells.
Song Ju-Ha et al prepared an apatite-collagen film by freeze-drying method as a guided bone tissue regeneration film (J Biomed. The porous structure of cell adhesion meets its biological performance requirements, but the degradation performance of the pure frozen membrane needs to be further improved, and the lack of flexibility is not conducive to operation
With the development of electrospinning technology, Yang Fang et al. used electrospinning technology to prepare n-HA / PCL membrane (Acta Biomaterialia, 2009, 5, 3295-3304), although in vitro experiments showed that it is beneficial to the formation of bone-like apatite. Deposition and cell adhesion, and good adhesion, but the mechanical properties of pure electrospun membranes are poor, and the mechanical properties and degradation properties of PCL itself are not ideal
Chinese patent CN101391113A discloses a composite film of polyurethane and nano-apatite prepared by casting method. Although a film with a dense surface and a loose surface can be obtained, it can be used as a membrane for guiding bone tissue regeneration, but this method is limited to polyurethane The preparation of base polymers has certain limitations for other polymers

Method used

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  • Composite film for guiding bone tissue regeneration and preparation method of composite film

Examples

Experimental program
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Effect test

Embodiment 1

[0023] Example 1: After dissolving 2g of L-PLCA (the molar ratio of LA and CL is 50:50, and the molecular weight is 50,000) in 40ml of acetone, cast it into a film, and dry it at room temperature to obtain a dense film with a thickness of 0.25mm. Then 1.0g L-PLCA (the molar ratio of LA and CL is 70:30, and the molecular weight is 250,000) is added to 10ml of dichloromethane to dissolve, and 0.15g of unmodified n-HA nanoparticles (average particle diameter is 60nm ) with 5ml N, after N dimethylformamide ethanol ultrasonic dispersion, electrospun on above-mentioned casting film and be the film that total thickness is 0.35mm, the fiber of electrospun film is about average 500nm to constitute, and the tensile strength of film is 22MPa, and the elongation at break is 470%. The film was immersed in simulated body fluid for 12 hours, and its surface was completely covered by bone-like apatite as observed by SEM.

Embodiment 2

[0024] Example 2: Add 2g of L-PLCA (the molar ratio of LA to CL is 50:50, and the molecular weight is 300,000) into 50ml of N, N dimethylformamide to dissolve it, cast it into a film, and dry it at room temperature to obtain the thickness It is a dense film of 0.28mm. After adding 1.0g of L-PLCA (the molar ratio of LA to CL is 80:20, and the molecular weight is 200,000) into 6ml of dichloromethane for dissolution, 0.5g of unmodified β-TCP nanoparticles (average particle diameter of 500nm ) after ultrasonic dispersion with 9ml N, N dimethylformamide ethanol, electrospun on the above-mentioned casting film to form a film with a total thickness of 0.37mm, and the electrospun film is composed of fibers with an average thickness of 650nm. The tensile strength of the film is 27MPa, and the elongation at break is 760%. The film is soaked in simulated body fluid for 10 hours, and its surface has been completely covered by bone-like apatite as observed by SEM.

Embodiment 3

[0025]Example 3: 2g of L-PLCA (the molar ratio of LA and CL is 70:30, the molecular weight is 250,000) was dissolved in 35ml of acetone, cast into a film, and dried at room temperature to obtain a dense film with a thickness of 0.26mm. After adding 1.0gL-PLCA (the molar ratio of LA and CL is 70:30, and the molecular weight is 300,000) to 6ml of dichloromethane for dissolution, 0.8g of modified n--HA nanoparticles (average particle diameter is 350nm) After ultrasonically dispersing with 12ml of N, N dimethylformamide ethanol, electrospun on the cast film above to form a film with a total thickness of 0.35mm, the electrospun film is composed of fibers with an average thickness of 830nm. The tensile strength of the film is 30MPa. The elongation at break is 650%. The film is immersed in simulated body fluid for 10 hours, and its surface has been completely covered by bone-like apatite as observed by SEM.

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Abstract

The invention relates to a composite film for guiding bone tissue regeneration and a preparation method of the composite film. The composite film is a double-layer film, wherein a bottom-layer film is an L-lactide and caprolactone copolymer (L-PLCA) cast film with a compact structure, and the molar ratio of monomer components is (50:50) to (95:05); a surface layer is an L-PLCA and hydroxyapatite (HA) or tricalcium phosphate (beta-TCP) compound electrospinning film with a micropore structure, wherein the mass ratio of HA or beta-TCP in the electrospinning film is 10-50wt%, and the particle size of the electrospinning film can be nano-grade or micro-grade. The double-layer composite film is relatively good in mechanical performance and bone-like apatite deposition induction capacity, favorable in flexibility and capable of realizing performance regulation through regulating the molar ratio of L-PLCA, the content of the component HA or beta-TCP and the microstructure of the electrospinning film so as to be a novel degradable double-layer composite film capable of guiding bone tissue regeneration.

Description

technical field [0001] The invention relates to a component of a composite film capable of guiding bone tissue regeneration and a preparation method thereof, belonging to the field of biomedical materials. Background technique [0002] In recent years, guided bone tissue regeneration membrane technology has provided a new effective way in the treatment of periodontal disease, insufficient bone mass in dental implant areas, repair of other bone defects, and fracture healing. The guiding bone tissue regeneration membrane is placed in the defect area, which can be used as a mechanical barrier membrane to selectively separate the surrounding soft tissue, prevent epithelial cells and fibroblasts from growing to the defect, and create a special regeneration space under the membrane , so that the bone-forming cells grow preferentially, generate new bone tissue and achieve the purpose of promoting bone healing. [0003] In the process of guided bone regeneration, the selection of m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L31/12A61L31/06A61L31/02A61L31/14
Inventor 蒋柳云徐莉娟熊成东陈栋梁熊左春庞秀炳
Owner CHENGDU ORGANIC CHEM CO LTD CHINESE ACAD OF SCI
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