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Modified tussah silk fibroin 3D printing support and preparation method thereof

A tussah silk fibroin, 3D printing technology, applied in the field of biological 3D printing, can solve the problem of not being able to take into account the mechanical properties of the scaffold and the biocompatible cell adhesion and proliferation ability at the same time, and achieve the effect of improving the survival rate and proliferation rate.

Active Publication Date: 2020-03-06
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0015] The purpose of the present invention is to provide a modified tussah silk fibroin 3D printing scaffold and its preparation method

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067] A method for preparing a modified tussah silk fibroin 3D printing scaffold, the main steps are as follows:

[0068] (1) Prepare tussah silk fibroin pulp:

[0069] (1.1) According to the mass volume ratio of 1g:50mL, the tussah cocoon is immersed in the Na with a mass concentration of 0.50wt%. 2 CO 3 In the aqueous solution, boil 3 times for 45 minutes each time, then wash the boiled tussah cocoons with water and dry to obtain degummed tussah silk fibers;

[0070] (1.2) Immerse the degummed tussah silk fiber in the formic acid aqueous solution of 90wt% by mass volume ratio of 1g:20mL for 2h;

[0071] (1.3) Homogenize at a temperature of 25°C for 2 minutes for pulverization, and the homogenate rate is 10000rpm;

[0072] (1.4) Repeat step (1.2) and step (1.3) 3 times to obtain tussah silk fibroin protein slurry;

[0073] (1.5) centrifuging, filtering, washing and drying the tussah silk fibroin protein slurry to obtain tussah silk fibroin protein pulp;

[0074] (2) Pre...

Embodiment 2

[0094] A method for preparing a modified tussah silk fibroin 3D printing scaffold, the steps of which are basically the same as in Example 1, except that the p-aminobenzoic acid modifier is replaced by the p-aminoacetophenone modifier.

[0095] The finally prepared modified tussah silk fibroin nanofibers have an aspect ratio of 146 and an average diameter of 86nm.

[0096] The extrusion swell rate of the core part printing ink is 0.52%, and the dynamic viscosity is 390cP.

[0097] The self-gelling time of the printing ink in the shell part is 39s, the extrusion swell rate is 12%, and the dynamic viscosity is 3500cP.

[0098] The final modified tussah silk fibroin 3D printing scaffold has a resolution of 5.6 μm and a compressive modulus of 319 MPa after soaking and cross-linking in 0.5 wt% genipin aqueous solution for 24 hours;

[0099] If 1 mL of fetal bovine serum culture medium containing 10,000 second-generation iPSCs was inoculated on a scaffold with a diameter of 15 mm a...

Embodiment 3

[0101] A method for preparing a modified tussah silk fibroin 3D printing scaffold, the steps of which are basically the same as in Example 2, except that the p-aminoacetophenone modifier is replaced by a 4-(2-ethylamino) aniline modifier.

[0102] The finally prepared modified tussah silk fibroin nanofibers have an aspect ratio of 111 and an average diameter of 126 nm.

[0103] The extrusion swell rate of the core part printing ink is 0.62%, and the dynamic viscosity is 350cP.

[0104] The self-gelling time of the printing ink in the shell part is 42s, the extrusion swell rate is 16%, and the dynamic viscosity is 2900cP.

[0105] The resolution of the final modified tussah silk fibroin 3D printing scaffold was 8.2 μm, and the compressive modulus was 289 MPa after immersion and cross-linking reaction in 0.5wt% genipin aqueous solution for 24 hours;

[0106] If 1 mL of fetal bovine serum culture medium containing 10,000 second-generation iPSCs was inoculated on a scaffold with ...

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Abstract

The invention relates to a modified tussah silk fibroin 3D printing support and a preparation method thereof. Core part printing ink prepared by using chemically modified tussah silk fibroin nano microfibers and shell part printing ink prepared by using a chemically modified tussah silk fibroin nano microfiber / gelatin composite system are used for carrying out 3D printing to prepare a modified tussah silk fibroin 3D printing support. The compression modulus of the modified tussah silk fibroin 3D printing support is 100 to 600 MPa after soaking in genipin with the mass concentration of 0.1 to 5wt% and subjection to a cross-linking reaction for 24 h; and after culture for 10 days, the survival rate and the proliferation rate of induced pluripotent stem cells are high. The finally prepared modified tussah silk fibroin 3D printing support comprises printing lines with core-shell structures. According to the invention, the preparation method is relatively simple; and the prepared 3D printing support has excellent mechanical properties, excellent biocompatibility and good tissue repair capability.

Description

technical field [0001] The invention belongs to the technical field of biological 3D printing, and relates to a modified tussah silk fibroin 3D printing support and a preparation method thereof. Background technique [0002] Tissue and organ defects and dysfunctions caused by trauma, tumors and aging are important factors that endanger human health, and the repair and functional reconstruction of defective tissues and organs is currently a difficult problem facing the world. Large-area defects usually need to be repaired by autologous or allogeneic tissue or organ transplantation. Autologous transplantation has the problem of "repairing wounds with trauma". For allogeneic transplantation, there are also major shortcomings such as insufficient donor sources and immune rejection. . The proposal, establishment and development of tissue engineering provide a new way to solve the problems of such tissue and organ defects and dysfunction. From the idea of ​​implanting cells on b...

Claims

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Application Information

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IPC IPC(8): A61L27/22A61L27/50B33Y10/00B33Y70/00B33Y80/00
CPCA61L27/222A61L27/227A61L27/50A61L2400/12B33Y10/00B33Y70/00B33Y80/00C08L89/00
Inventor 邵惠丽邹盛之张耀鹏王心如张佳明范苏娜胡学超
Owner DONGHUA UNIV
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