Modified tussah silk fibroin 3D printing support and preparation method thereof
A tussah silk fibroin, 3D printing technology, applied in the field of biological 3D printing, can solve the problem of not being able to take into account the mechanical properties of the scaffold and the biocompatible cell adhesion and proliferation ability at the same time, and achieve the effect of improving the survival rate and proliferation rate.
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[0066] Example 1
[0067] A preparation method of modified tussah silk fibroin 3D printing scaffold, the main steps are as follows:
[0068] (1) Preparation of tussah silk fibroin pulp:
[0069] (1.1) Immerse tussah cocoons into Na with a mass concentration of 0.50wt% according to the mass-volume ratio of 1g:50mL 2 CO 3 In the aqueous solution, boil 3 times for 45 minutes each time, then wash the boiled tussah cocoons with water and dry to obtain degummed tussah silk fibers;
[0070] (1.2) Immerse the degummed tussah silk fiber in a mass-to-volume ratio of 1g:20mL in a formic acid aqueous solution with a mass concentration of 90wt% for 2h;
[0071] (1.3) The homogenization treatment is carried out for 2 minutes at a temperature of 25°C, and the homogenization rate is 10000 rpm;
[0072] (1.4) Repeat step (1.2) and step (1.3) 3 times to obtain tussah silk fibroin slurry;
[0073] (1.5) Centrifuging, filtering, washing and drying the tussah silk fibroin slurry to obtain tussah silk fibroin ...
Example Embodiment
[0093] Example 2
[0094] A method for preparing a modified tussah silk fibroin 3D printing scaffold. The steps are basically the same as those in Example 1, except that the para-aminobenzoic acid modifier is replaced with a para-aminoacetophenone modifier.
[0095] The final modified tussah silk fibroin nanofibers have an aspect ratio of 146 and an average diameter of 86 nm.
[0096] The swelling rate of the core printing ink is 0.52%, and the dynamic viscosity is 390cP.
[0097] The self-gelling time of the printing ink on the shell part is 39s, the extrusion swelling rate is 12%, and the dynamic viscosity is 3500 cP.
[0098] The final prepared modified tussah silk fibroin 3D printed scaffold has a resolution of 5.6 μm, and a compression modulus of 319 MPa after being immersed in a 0.5 wt% genipin aqueous solution for 24 hours after cross-linking reaction;
[0099] If 1 mL of fetal bovine serum culture medium containing 10,000 second-generation iPSCs is inoculated on a stent with a di...
Example Embodiment
[0100] Example 3
[0101] A method for preparing a modified tussah silk fibroin 3D printing scaffold. The steps are basically the same as those in Example 2, except that 4-(2-ethylamino)aniline modifier is used to replace p-aminoacetophenone modifier.
[0102] The final modified tussah silk fibroin nanofibers have an aspect ratio of 111 and an average diameter of 126 nm.
[0103] The swelling rate of the core printing ink is 0.62%, and the dynamic viscosity is 350cP.
[0104] The self-gelling time of the printing ink on the shell part is 42s, the extrusion swelling rate is 16%, and the dynamic viscosity is 2900cP.
[0105] The final prepared modified tussah silk fibroin 3D printed scaffold has a resolution of 8.2μm, and a compression modulus of 289MPa after being immersed in a 0.5wt% genipin aqueous solution for 24h after cross-linking reaction;
[0106] If 1 mL of fetal bovine serum culture medium containing 10,000 second-generation iPSCs is inoculated on a stent with a diameter of 15 m...
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