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Degradable nano-short fiber material for tissue repair as well as preparation method and applications of degradable nano-short fiber material

A tissue repair and nanofiber technology, which is applied in the field of degradable nanofiber materials for tissue repair and its preparation, can solve the problems of unfavorable biomaterial biocompatibility, inability to disperse or disperse, difficult to apply, etc. Effect of fiber deformation, avoidance of voids, good dispersion properties and repair properties

Active Publication Date: 2016-11-09
MEDPRIN REGENERATIVE MEDICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the nanofibers prepared by electrospinning are generally continuous in length, unable to disperse or have poor dispersibility, and are difficult to apply in small and deep defects or minimally invasive surgery; therefore, it is necessary to study a good dispersibility Short nanofibers that can be used in smaller or deeper defects or in minimally invasive procedures
[0004] At present, the preparation methods of short nanofibers generally include direct methods such as template method, hydrothermal growth method, chemical vapor deposition method, or post-treatment method of electrospinning plus calcination (Law M, Goldberger J, Yang P. Semiconductorrnanowires and nanotubes[J ]. Annu. Rev. Mater. Res., 2004, 34: 83-122.), etc., but these methods are mainly used in the research of metals, inorganic non-metallic materials and the field of biosensors, and they will use high temperature in the preparation process Or too many organic reagents are not conducive to the maintenance of biocompatibility of biological materials, and cannot be used to prepare short nanofibers for the field of tissue repair

Method used

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  • Degradable nano-short fiber material for tissue repair as well as preparation method and applications of degradable nano-short fiber material
  • Degradable nano-short fiber material for tissue repair as well as preparation method and applications of degradable nano-short fiber material
  • Degradable nano-short fiber material for tissue repair as well as preparation method and applications of degradable nano-short fiber material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0071] Embodiment 1 Preparation of polylactic acid nano short fiber material

[0072] S1. Add 0.8g of polylactic acid into 10ml of hexafluoroisopropanol solution, stir at room temperature until dissolved, and make 8% (w / v) spinning solution; use deionized water as the solid liquid;

[0073] S2. Add the spinning solution into the syringe, add an extension tube to the front end of the syringe and connect a 20G needle, place the syringe on the micro-injection pump, the needle is perpendicular to the receiving plate, and the lower part of the receiving plate is grounded; set the injection rate to 2ml / h, when When the solution is extruded from the needle tip, a voltage of 22kv is applied to the needle tip; at this time, nanofibers are ejected and collected on the receiving plate to form a nanofiber film;

[0074] S3. Vacuum-dry the nanofibrous membrane for 48 hours to remove hexafluoroisopropanol, then transfer it to the solid liquid, repeatedly vacuum until it sinks to the bottom ...

Embodiment 2

[0077] Embodiment 2 Preparation of silk fibroin nano-short fiber material

[0078] S1. Add 1g of silk fibroin to 10ml of formic acid solution, stir at room temperature until dissolved, and make a 10% (w / v) spinning solution; use ethanol as the solid liquid and coagulation bath;

[0079] S2. Put the spinning solution into the syringe, add an extension tube to the front of the syringe and connect a 20G needle, place the syringe on the micro-injection pump, the needle is perpendicular to the crystallization dish, the crystallization dish is filled with the coagulation bath, and the lower part is grounded; set the injection rate 1ml / h, when the needle tip is extruded with a solution, a voltage of 18kv is applied to the needle tip; at this time, nanofibers are ejected and collected in the coagulation bath to form nanofiber clusters;

[0080] S3. In the case of replacing ethanol every 12 hours, soak the nanofibrous cluster in the coagulation bath for 48 hours to remove formic acid, ...

Embodiment 3

[0083] Example 3 Preparation of polyvinyl alcohol nano short fiber material

[0084] S1. Add 1g of polyvinyl alcohol into 10ml of deionized water, stir at 90°C until dissolved, and make a 10% (w / v) spinning solution; use methanol as the solid liquid;

[0085] S2. Add the spinning solution into the syringe, add an extension tube to the front end of the syringe and connect a 20G needle, place the syringe on the micro-injection pump, the needle is perpendicular to the receiving plate, and the receiving plate is grounded; set the injection rate to 1ml / h, when the needle tip When the solution is extruded, a voltage of 25kv is applied to the needle tip; at this time, nanofibers are ejected and collected on the receiving plate to form a nanofiber film;

[0086] S3. Since the solvent of polyvinyl alcohol is water, no additional removal is required, and it is directly transferred to the solid liquid, vacuumized, soaked for 5 minutes, and then taken out;

[0087] S4. Transfer the nanof...

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Abstract

The invention relates to a degradable nano-short fiber material for tissue repair. The nano-short fiber material is composed of nano-short fibers, wherein the diameter of the nano-short fibers is 200-800 nm, and the length of the nano-short fibers does not exceed 500 [mu] m; the length of at least 93% of the nano-short fibers in the nano-short fiber material is within 20-200 [mu] m, and the stacking density of the nano-short fiber material is 0.001-0.099 g / cm<3>. According to the nano-short fiber material, the length of the nano-fibers is shortened, the dispersing performance and repairing performance of the nano-fibers are improved, and the application range of the nano-short fiber material is expanded.

Description

technical field [0001] The invention relates to the technical field of tissue repair scaffolds, in particular to a degradable nano-short fiber material for tissue repair and its preparation method and application. Background technique [0002] Various tissues and organs of the human body may be abnormal or damaged. At present, the transplantation of tissues and organs is mainly used to restore their functions. However, there is a shortage of tissues and organs available for transplantation. Even if the transplantation is successful, long-term immunosuppressive treatment must be carried out later. Since 1960, researchers have attempted to use various biomaterials to construct scaffolds that can be used for tissue repair; in 1984 Wolter (Wolter J R, Meyer R F. Sessile macrophages forming clearendothelium-like membrane on inside of successful keratoprosthesis.[J]. the American Ophthalmological Society, 1984,82:187.) The term "tissue engineering" was first proposed. "Tissue en...

Claims

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Application Information

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IPC IPC(8): A61L27/18A61L27/22A61L27/16D01F4/00D01F6/14D01F6/34D01F6/62
CPCA61L27/16A61L27/18A61L27/22D01F4/00D01F6/14D01F6/34D01F6/625C08L67/04C08L29/04
Inventor 张海涛邓坤学袁玉宇
Owner MEDPRIN REGENERATIVE MEDICAL TECH
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