Cell culture medium, preparation method and application

Abstract

The invention provides a cell culture medium, a preparation method and application, and belongs to the technical field of cell culture. The cell culture medium is prepared from a basic culture medium,cytokines, hormones, proteins, biogenic amines, an inhibitor and solution declining elements, wherein the cytokines, the hormones, the proteins, the biogenic amines, the inhibitor and the solution declining elements are added into the basic culture medium. The basic culture medium can effectively maintain the stability of the cell environment while maintaining the activity of the cells. By addingthe cytokines, the hormones, the proteins, the biogenic amines, the inhibitor and the solution declining elements into the basic culture medium, on the one hand, through the synergy between the components, animal-derived components in a traditional culture medium can be replaced, the stability of the cell culture medium can be ensured, and the risks of disease transmission and immune response arereduced; and on the other hand, the components cooperate with one another other, the cell activity is maintained, cells can proliferate steadily and rapidly, the primary separation efficiency of thecells is greatly improved, and the self-renewal and tissue repair capabilities of the cells are improved.

Description

technical field [0001] The invention relates to the technical field of cell culture, in particular to a cell culture medium and its preparation method and application. Background technique [0002] In vitro cell culture is an important and commonly used technique in cell biology research, and its variables include culture medium (basal medium and supplements), culture surface matrix, cell seeding density and physicochemical environment, and subculture protocol. Among them, the medium can be said to be the most critical factor. Fetal bovine serum (FBS) is added to traditional culture media, and there is a potential risk of infection and immune response. To reduce these risks, efforts have been made to develop supplements of human origin, including human serum and platelet lysates. However, the use of supplements of human origin is rather controversial, mainly because of limited sources and the possibility of disease transmission between donor and patient. In addition, the ...

AI Technical Summary

Problems solved by technology

[0003] Currently, some commercial media are available on the market, but the composition of these commercial media is kept secret, so it is not clear whether their composition is fully defined

In addition, these commercial serum-free, animal-derived component-free media do not perform well in supporting the clinical culture of cells, especially stem cells, due to their poor performance.

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