An injectable decellularized fat-matrix microparticle and applications thereof in implants

A decellularization and implant technology, applied in the field of regenerative medicine, can solve the problems of loss of active ingredients, weakened tissue repair effect, injection and other problems, and achieve the effect of promoting tissue repair and regeneration, good tissue repair effect, and reducing immune rejection.

Inactive Publication Date: 2017-03-15
广州昕生医学材料有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The method for preparing microparticles in this patent is only to physically cut the crosslinked material into microparticles with a side length of 300-500 um. However, the particle size is too large to be injected with a needle with a smaller diameter; Practical value in the field of plastic surgery
[0005] Chinese patent CN02156797.2 discloses a preparation method of injectable collagen and its products and applications. This method uses collagen fibers in animal tissues to prepare injectable collagen. However, the original animal tissue will be destroyed during the preparation process. The structure and other components also lose the active components with biological activity or biological function in the tissue, and can only play a role of pure collagen filling
[0006] As mentioned above, some minimally invasive injection plastic implant materials that are currently on the market and the preparation methods of minimally invasive injection plastic implant materials disclosed in similar domestic patents have certain shortcomings, especially collagen and hyaluronic acid implant products. Contains bioactive factors, so its function is only filling. The effect of this type of implant product is not good. When these filling materials are gradually decomposed and absorbed by the body, the tissue repair effect will be weakened or disappeared.
At present, there is no product that uses decellularized fat as raw material to prepare injectable filling materials, and there are no patents and literature reports

Method used

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  • An injectable decellularized fat-matrix microparticle and applications thereof in implants
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  • An injectable decellularized fat-matrix microparticle and applications thereof in implants

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Effect test

Embodiment 1

[0048] The preparation method of injectable decellularized fat matrix microparticles comprises the following steps:

[0049]1) Take adipose tissue from pigs, rinse 3 times in PBS solution, sterilize the adipose tissue with 75% ethanol aqueous solution for 30 minutes, rinse 3 times in PBS solution, and then place the adipose tissue at -80 ℃ refrigerator for 2 hours, thawed at 37 ℃ for 0.5 hours, repeated freezing and thawing 3 times;

[0050] 2) Cut the obtained adipose tissue into thin slices with a thickness not greater than 2 mm. Soak the fat slices in pure water for 3 hours, and then rinse them in isopropanol for 36 hours. The volume ratio of fat slices to isopropanol solution is 1:80. Change isopropanol 1 time during the period; then rinse 3 times in PBS solution;

[0051] 3) Soak the obtained fat flakes in 0.01M sodium hydroxide aqueous solution containing 0.5MEDTA for 12h, the volume ratio of the fat flakes to the solution is 1:50, rinse with PBS solution for 3 times; t...

Embodiment 2

[0057] The preparation method of injectable decellularized fat matrix microparticles comprises the following steps:

[0058] 1) Take the adipose tissue of cattle, rinse it in PBS solution for 4 times, sterilize the fat with 0.5% peracetic acid aqueous solution for 30 minutes, rinse it in PBS solution for 3 times, put the adipose tissue in -80 ℃ refrigerator for 2 hours, thawed at 37 ℃ for 0.8 hours, repeated freezing and thawing 3 times;

[0059] 2) Cut the obtained adipose tissue into thin slices with a thickness not greater than 2 mm. Soak the fat slices in pure water for 3 hours, and then rinse them in isopropanol for 30 hours. The ratio of the fat slices to the solution is 1:50 by volume. During rinsing Change the medium once; then rinse 3 times in PBS solution;

[0060] 3) Soak the obtained fat flakes in 0.01M sodium hydroxide solution containing 1MEDTA for 14h, the volume ratio of the fat flakes to the solution is 1:60, rinse with PBS solution for 3 times; then place th...

Embodiment 3

[0066] The preparation method of injectable decellularized fat matrix microparticles comprises the following steps:

[0067] 1) Take adipose tissue from pigs, rinse it in PBS solution for 3 times, sterilize the adipose tissue with 75% ethanol aqueous solution for 10 minutes, and then sterilize the adipose tissue with 0.2% peracetic acid aqueous solution Sterilize for 20 minutes, rinse 3 times in PBS solution, then freeze the adipose tissue in -80°C refrigerator for 1.5h, and thaw at 37°C for 0.5h, repeat freezing and thawing 3 times;

[0068] 2) Cut the obtained adipose tissue into thin slices with a thickness not greater than 2 mm. Soak the fat slices in pure water for 2 hours, and then rinse them in petroleum ether-ethanol (volume ratio of petroleum ether and ethanol 1:2) for 36 hours. The fat slices and the solution The volume ratio of the solution is 1:40, the medium is changed twice during rinsing, and then rinsed 3 times with PBS solution;

[0069] 3) Soak the obtained ...

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Abstract

An injectable decellularized fat-matrix microparticle and applications thereof in implants are disclosed. The microparticle is prepared by rinsing, disinfecting and rising fat mass, performing repeated freezing-thawing, cutting the fat mass into slices, soaking the slices, degreasing, decellularizing, performing freeze-drying, and performing low-temperature grinding. The microparticle can be cooperated with an implant auxiliary and adopted as an injectable decellularized fat-matrix microparticle implant. The microparticle has advantages of good biocompatibility, capability of promoting tissue regeneration, and the like. As the microparticle has a three-dimensional structure, the microparticle can be adopted as a support for supporting tissue and cell growth. Active components of the microparticle can induce and regulate cell adhesion, growth, proliferation and differentiation and can promote tissue repairing and regeneration. The implant can be adopted as a minimally invasive plastic injection implanting material, and the implant has a filling function and a tissue repairing function as the natural three-dimensional structure and a lot of the active components of fat are preserved.

Description

technical field [0001] The invention belongs to the field of regenerative medicine, in particular to injectable decellularized fat matrix microparticles and their application in implants. Background technique [0002] The application of minimally invasive injection technology in the field of regenerative medicine is developing rapidly, and it is also gradually shown in the repair treatment of trauma, disease or congenital deformity and cosmetic plastic surgery (such as repair treatment of maxillofacial soft tissue defects, rhinoplasty and chin augmentation). It has the unique advantages of small trauma and quick recovery. Whether used alone or as a supplement to orthognathic and orthodontic repair operations, minimally invasive injection technology has broad application prospects in trauma reconstruction, repair of maxillofacial soft tissue defects, rhinoplasty and chin augmentation. [0003] Bovine collagen has been approved by the FDA as an injection filling material. Zy...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/54A61L27/50A61L27/36A61L27/20A61L27/18
CPCA61L27/18A61L27/20A61L27/3604A61L27/3683A61L27/50A61L27/54A61L2300/252A61L2400/06A61L2430/40C08L5/04C08L5/08C08L77/04
Inventor 杨伟红程飚王祥林陈大鹏邓小敏刘江辉
Owner 广州昕生医学材料有限公司
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