Injectable decellularized small intestinal submucosal matrix particles and preparation method and application thereof

A small intestinal submucosa and decellularization technology, applied in the field of regenerative medicine, can solve the problems of disappearing effect, destroying animal tissue structure and other components, injection, etc., to achieve good structural characteristics, good tissue repair effect, and reduce the effect of immune rejection

Inactive Publication Date: 2018-04-20
广州昕生医学材料有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The method of preparing microparticles in this patent is only to physically cut the crosslinked material into microparticles with a side length of 300-500 μm. However, the particle size is too large to be injected with a needle with a smaller diameter, which limits its application in the field of injection plastic surgery. practical value
[0005] Chinese patent CN02156797.2 discloses a preparation method of injectable collagen and its products and applications. The method uses collagen fibers in animal tissues to prepare injectable collagen. However, the original animal tissue will be destroyed during the preparation process. The structure and other components also lose the active components with biological activity or biological function in the tissue, and can only simply play the role of collagen filling
[0007] The existing technology products will play a certain role of filling and anti-wrinkle in the time when they are not degraded after injection, but this material will be gradually degraded and absorbed by the relevant enzymes of the body as the implantation time prolongs, because it does not exist active ingredient, so the effect also wears off

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  • Injectable decellularized small intestinal submucosal matrix particles and preparation method and application thereof
  • Injectable decellularized small intestinal submucosal matrix particles and preparation method and application thereof
  • Injectable decellularized small intestinal submucosal matrix particles and preparation method and application thereof

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Embodiment 1

[0038] An embodiment of the injectable acellular small intestinal submucosa matrix particles and its preparation method of the present invention, the preparation method of the injectable acellular small intestinal submucosa matrix particles described in this embodiment is:

[0039] (1) Take the mammalian small intestine tissue, rinse it with a buffer solution, remove the blood and small intestine content, put it into a disinfectant prepared with sterile water for injection, soak and sterilize it, and then perform repeated freeze-thaw treatment; wherein, the The disinfectant is composed of ethanol aqueous solution with a volume fraction of 5-30% and a peracetic acid aqueous solution with a volume fraction of 0.2-2%. The method of repeated freezing and thawing is as follows: the animal small intestine tissue is placed in a -80°C refrigerator for 1.5 hours , then take it out, and thaw at 37°C for 0.5 hours; the number of times of repeated freezing and thawing is 3 times;

[0040]...

Embodiment 2

[0047] An embodiment of the injectable acellular small intestinal submucosa matrix particles and its preparation method of the present invention, the preparation method of the injectable acellular small intestinal submucosa matrix particles described in this embodiment is:

[0048] (1) Take the mammalian small intestine tissue, rinse it with a buffer solution, remove the blood and small intestine content, put it into a disinfectant prepared with sterile water for injection, soak and sterilize it, and then perform repeated freeze-thaw treatment; wherein, the The disinfectant is composed of ethanol aqueous solution with a volume fraction of 5-30% and a hydrogen peroxide aqueous solution with a volume fraction of 0.5-10%; the method of repeated freezing and thawing is: placing the animal small intestine tissue in a -80°C refrigerator for 0.5 hours , then take it out, and thaw at 37°C for 0.1 hour; the number of times of repeated freezing and thawing is 5 times.

[0049] (2) Cut o...

Embodiment 3

[0056] An embodiment of the injectable acellular small intestinal submucosa matrix particles and its preparation method of the present invention, the preparation method of the injectable acellular small intestinal submucosa matrix particles described in this embodiment is:

[0057] (1) Take the mammalian small intestine tissue, rinse it with a buffer solution, remove the blood and small intestine content, put it into a disinfectant prepared with sterile water for injection, soak and sterilize it, and then perform repeated freeze-thaw treatment; wherein, the The disinfectant is composed of 5-30% ethanol aqueous solution, 0.2-2% peracetic acid aqueous solution and 0.5-10% hydrogen peroxide aqueous solution by volume fraction; the method of repeated freezing and thawing is: Animal small intestine tissue was frozen in a -80°C refrigerator for 3 hours, then taken out, and thawed at 37°C for 1 hour; the number of repeated freezing and thawing was 2 times.

[0058] (2) Cut off the sm...

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Abstract

The invention relates to injectable decellularized small intestinal submucosal matrix particles and a preparation method and the application thereof, and belongs to the field of regenerative medicine.The preparation method of the injectable decellularized small intestinal submucosal matrix particles comprises the following steps: (1), taking an animal small intestine tissue, and sequentially rinsing, sterilizing and repeatedly freezing and thawing; (2), shearing the animal small intestine tissue, scraping off a mucosal layer, a muscle layer and a serosa layer of the small intestine to obtaina stripped small intestine submucosa, and soaking the stripped small intestine submucosa in a sodium chloride solution; (3), decellularizing the small intestine submucosa, and then rinsing; (4), soaking the small intestine submucosa in a buffer solution, then putting into a descaling agent, extracting while shaking, and rinsing; (5), soaking the small intestine submucosa in the buffer solution, performing freeze drying, and grinding to obtain the injectable decellularized small intestinal submucosal matrix particles. The injectable decellularized small intestinal submucosal matrix particles prepared by the preparation method provided by the invention are rich in bioactive component and structurally loose and porous.

Description

technical field [0001] The invention relates to an injectable decellularized small intestinal submucosa matrix particle, a preparation method and application thereof, and belongs to the field of regenerative medicine. Background technique [0002] Injection micro-plastic surgery is a form of micro-plastic surgery. It is sought after by people for its advantages of minimally invasive, painless and short recovery period. It is a plastic surgery item that has become popular in recent years. It has also gradually shown its unique advantages of less trauma and faster recovery in the repair treatment of maxillofacial soft tissue defects, rhinoplasty and chin augmentation. Whether it is used alone or as a supplement to orthognathic and orthodontic repair surgery, minimally invasive injection technology has broad application prospects in trauma reconstruction, repair of maxillofacial soft tissue defects, rhinoplasty and chin augmentation. [0003] At present, the main materials tha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36A61L27/56A61L27/50A61L27/54
CPCA61L27/3629A61L27/50A61L27/54A61L27/56A61L2300/30A61L2300/412A61L2430/34A61L2430/40
Inventor 杨伟红程飚王祥林耿梦梦晏兰苏玉天刘江辉
Owner 广州昕生医学材料有限公司
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