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Preparation method of porous nano-fiber tubular scaffold

A nanofiber and tubular technology, which is applied in the field of preparation of tubular tissue engineering scaffolds, can solve the problems of high brittleness of PLLA scaffolds and difficulty in providing toughness and elasticity of tubular tissues, and achieve low equipment requirements, improved toughness and elasticity, and simple preparation process Effect

Inactive Publication Date: 2013-02-06
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

S. Ramakrishna et al. (Polymer, 2009, 50: 4128-4138) used dioxane and water as a mixed solvent to dissolve PLLA, and prepared a macroporous nanofiber scaffold by thermally induced phase separation, but the pure PLLA scaffold is relatively brittle. It is difficult to provide the necessary mechanical strength such as toughness and elasticity in the process of common tubular tissue repair

Method used

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  • Preparation method of porous nano-fiber tubular scaffold

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] The nanofibrous tubular scaffold with porous structure was prepared under the condition of mass ratio PLLA:PCL=70:30 and total polymer concentration of 0.05 g / mL.

[0033] (1) Dissolve PLLA and PCL in tetrahydrofuran at 55°C at a ratio of 70:30 to form a homogeneous solution;

[0034] (2) Inject the polymer solution into the polytetrafluoroethylene mold, and quickly place it at -80°C to allow phase separation to occur overnight;

[0035] (3) Take it out from the low temperature, and remove the shell of the mold, soak the gelled polymer and the core mold in deionized ice water for 30 minutes, take out the core mold, and soak the polymer in deionized Exchange the solvent in ice water for 2 days, and change the deionized ice water three times a day;

[0036] (4) The tubular scaffold is taken out from the deionized water and freeze-dried to obtain a nanofiber tubular scaffold with a porous structure.

Embodiment 2

[0038] The porous nanofibrous tubular scaffold was prepared under the condition of mass ratio of PLLA:PU=70:30 and total polymer concentration of 0.1g / mL.

[0039] (1) Dissolve PLLA and PU in tetrahydrofuran at 60°C at a ratio of 70:30 to form a homogeneous solution;

[0040] (2) Inject the polymer solution into the polytetrafluoroethylene mold, and quickly place it at -100°C to allow phase separation to occur overnight;

[0041] (3) Take it out from the low temperature, and remove the shell of the mold, soak the gelled polymer and the core mold in deionized ice water for 30 minutes, take out the core mold, and soak the polymer in deionized Exchange the solvent in ice water for 3 days, and change the deionized ice water three times a day;

[0042] (4) The tubular scaffold is taken out from the deionized water and freeze-dried to obtain a nanofiber tubular scaffold with a porous structure.

Embodiment 3

[0044] The porous nanofiber tubular scaffold was prepared under the condition of mass ratio of PLLA:PLGA=60:40 and total polymer concentration of 0.15g / mL.

[0045] (1) Dissolve PLLA and PLGA in tetrahydrofuran at 70°C at a ratio of 60:40 to form a homogeneous solution;

[0046] (2) Inject the polymer solution into the polytetrafluoroethylene mold, and quickly place it at -50°C to allow phase separation to occur overnight;

[0047] (3) Take it out from the low temperature, and remove the shell of the mold, soak the gelled polymer and the core mold in deionized ice water for 30 minutes, take out the core mold, and soak the polymer in deionized Exchange the solvent in ice water for 3 days, and change the deionized ice water three times a day;

[0048] (4) The tubular scaffold is taken out from the deionized water and freeze-dried to obtain a nanofiber tubular scaffold with a porous structure.

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Abstract

The invention relates to a preparation method of a porous nano-fiber tubular scaffold, which comprises the steps that (1) PLLA (Poly L Lactic Acid) and other polymers are dissolved in a solvent to get a polymer solution; (2) the polymer solution is injected into a tubular mold and rapidly placed at a low temperature for phase separation, then the polymer solution is taken out, a tubular mold housing is removed, polymer gel after the phase separation and a core mold are immersed in ice water together, then the core mold is taken out, the polymer gel is immersed in deionized ice water to exchange the solvent, and the tubular scaffold is obtained; and (3) finally, the tubular scaffold obtained is frozen and dried for 48-120 hours, and then the porous nano-fiber tubular scaffold is obtained. The preparation method is simple to operate, and requires no additional hole-foaming agent, is suitable for volume production, and is lower in preparation cost; the prepared tubular scaffold has a nano-fiber structure similar to an extracellular matrix of a human tissue, and a porous structure with the diameter and porosity capable of being adjusted, and facilitates growth of cells and reconstruction of a cambium.

Description

technical field [0001] The invention belongs to the field of preparation of tubular tissue engineering scaffolds, in particular to a preparation method of porous nanofiber tubular scaffolds. Background technique [0002] The human body contains many luminal tissues, such as cardiovascular, peripheral blood vessels, nerve conduits, esophagus, intestinal tract, bile duct, urethra, and bone, etc. Tubular tissue scaffolds are often used to repair the defects of these tissues. Among the lesions of these tubular tissues, cardiovascular disease is one of the most serious diseases threatening human health. Transplantation is usually required for defective and severely diseased blood vessels. At present, autologous blood vessels such as the great saphenous and internal mammary arteries are commonly used as grafts in clinical practice. However, the source of autologous blood vessels is limited, and autologous transplantation is likely to cause secondary damage to the human body. All...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61F2/04A61L27/18A61L27/24A61L27/22A61L27/20A61L27/16A61L27/56
Inventor 何创龙胡金伟程晓王伟忠
Owner DONGHUA UNIV
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