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Application of amino acid molecular combination as stomach cancer marker

An amino acid and molecular technology, applied in material separation, analytical materials, measuring devices, etc., can solve the problems of difficult unification of sensitivity and specificity, and achieve the effect of simple operation, good clinical application value, and less time-consuming

Active Publication Date: 2015-05-20
RUIJIN HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a gastric cancer detection marker suitable for large-scale screening and non-invasive detection, to overcome the sensitivity and specificity of existing clinical detection tumor markers such as CA125, CA199, CA724, AFP, CEA and other markers Difficult to unify and other issues

Method used

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  • Application of amino acid molecular combination as stomach cancer marker
  • Application of amino acid molecular combination as stomach cancer marker
  • Application of amino acid molecular combination as stomach cancer marker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1: Apparatus, reagents, sample preparation and pretreatment.

[0033] Reagents and instruments: L-2-chlorophenylalanine, ethyl chloroformate, pyridine, absolute ethanol, sodium hydroxide, chloroform, anhydrous sodium sulfate, alanine (Alanine, Ala), glycine (Glycine, Gly ), valine (Valine, Val), serine (Serine, Ser), isoleucine (Iso), threonine (Threonine, Thr), proline (Proline, Pro), methionine (Methionine, Met), Tyrosine (Tyrosine, Tyr), Trytophan (Trytophan, Try) and other reagents are all analytical pure, produced by Shanghai Sinopharm Chemical Reagent Company. KQ-250DB CNC ultrasonic cleaner (produced by Kunshan Ultrasonic Instrument Co., Ltd.). LD5-2A desktop centrifuge (Beijing Jingli Centrifuge Co., Ltd.). The gas chromatograph mass spectrometer TurboMass (7890A GC / 5975C MS from Agilent, USA) comes with NIST2011 spectral library and chemical software workstation.

[0034] Subject's samples: 200 urine samples were randomly collected, of which 89 were healt...

Embodiment 2

[0036] Example 2: Setting of instrument scanning method.

[0037] The scanning mode is full ion scan combined with selected ion (SIM ion) monitoring scan. SIM ions are set to 44m / z for alanine, 102m / z for glycine, 144m / z for valine, 132m / z for serine, 158m / z for isoleucine, 101m / z for threonine, and 142m / z for proline , Methionine 61m / z, Tyrosine 107m / z, Tryptophan 130m / z, 2-Chlorophenylalanine 102m / z. The sample is first applied to a gas chromatography capillary column (Agilent J&WDB-5MS, 30m×0.25 mm×0.25μm). And using temperature-programmed gas chromatography technology, the starting temperature is 80°C, the temperature is increased to 140°C at a rate of 10°C / min, and then to 280°C at a rate of 4°C / min, and maintained for 2 minutes. Gradually increase the column temperature during the separation process so that all components can be eluted at their optimal temperature. The electron bombardment energy of the mass spectrometer is 70eV, the ion source temperature is 200°C, the ...

Embodiment 3

[0038] Example 3: Establishment of a standard quality spectrum and a standard curve.

[0039] Prepare 10 kinds of biomarker standard mixed solutions, dilute them into 10 concentration gradients in sequence, and pretreat them according to the method in Example 1, and inject the samples in Example 2. The results are shown in Table 1. The standard curve of each biomarker has good linearity, which serves as the basis for quantification. See the retention time of each biomarker figure 1 (The serial numbers are 1: Alanine; 2: Glycine; 3: Valine; 4: Serine; 5: Isoleucine; 6: Threonine; 7: Proline; 8: Methionine; 9: Tyrosine; 10: Tryptophan; Internal standard: 2-Chlorophenylalanine), the corresponding mass spectrum is shown in 2 as a qualitative basis.

[0040] Table 1 Each target molecule retention time (RT), standard curve linear range (Linear range) and linear fit (r 2 )

[0041]

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Abstract

The invention relates to the technical field of biological detection, in particular to application of an amino acid molecular combination as a stomach cancer marker. The invention discloses application of an amino acid molecular combination as a stomach cancer marker in preparation of stomach cancer detection kits. The amino acid molecular combination is composed of alanine, glycine, valine, serine, threonine, proline, methionine and tyrosine. As a stomach cancer marker, the amino acid combination provided by the invention has the characteristics of very good sensitivity and specificity, simple operation and little time. At the same time, the detection sample adopted by the invention is urine, thus avoiding physical pain brought to patients due to use of tissue and blood samples. Therefore, no matter from patient compliance or from the accuracy of detection results, the amino acid combination provided by the invention as the stomach cancer marker, especially an early stomach cancer marker has very good clinical application value.

Description

Technical field [0001] The technical field of biological detection of the present invention specifically relates to the use of a combination of amino acid molecules as gastric cancer markers. Background technique [0002] As the second most common malignant tumor in the world, gastric cancer is also one of the most common malignant tumors in my country, and it accounts for the first cause of death from malignant tumors of the digestive tract. The incidence of gastric cancer in my country is about 20 / 100,000, 400,000 new gastric cancer patients and 300,000 deaths each year, and the proportion of young people suffering from gastric cancer is increasing. 15% of the patients are young people under 40. Most patients with gastric cancer are accompanied by non-specific upper gastrointestinal discomfort symptoms. Therefore, large-scale screening of symptomatic populations can increase the detection rate of gastric cancer and improve the screening efficiency. However, some studies sugges...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88
Inventor 于颖彦陈逸南朱正纲
Owner RUIJIN HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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