Diagnosis primer and kit for type 2 diabetes, and application of non-coding RNA molecular marker

Abstract

The invention relates to a diagnosis primer and kit for type 2 diabetes, and an application of a non-coding RNA molecular marker. The diagnosis primer comprises a miR-802 forward primer as shown in SEQ ID NO:3 and a miR-802 reverse primer as shown in SEQ ID NO:4; the diagnosis kit for type 2 diabetes comprises the diagnosis primer; the non-coding RNA molecular marker is has-miR-802, or has-miR-802and has-lncRNA-ROIT; and the above diagnosis primer and non-coding RNA molecular marker can diagnose diabetes. The present invention can diagnose type 2 diabetes by utilizing the diagnosis primer/thediagnosis kit and by detecting the non-coding RNA molecular marker. The detection method is simple to operate, high in sensitivity and high in specificity, has small trauma to a subject, can be usedfor diagnosing type 2 diabetes in early stage and different stages, and has obvious clinical application value.

Description

technical field [0001] The invention relates to a diagnostic primer for type 2 diabetes, and also relates to a kit containing the diagnostic primer and the application of non-coding RNA molecular markers, belonging to the fields of cell biology and biomedicine. Background technique [0002] Diabetes mellitus is a chronic disease with disturbance of glucose metabolism as the main symptom caused by multiple factors. Although diabetes is preventable and controllable, chronic vascular complications derived from diabetes, such as microangiopathy and macrovascular disease, peripheral vascular and cardiovascular disease, peripheral neuropathy, nephropathy, etc., seriously endanger the physical and mental health of patients, and also bring great harm to patients' families and individuals. bring a heavy mental and economic burden. Moreover, due to the complicated pathogenesis of diabetes, no cure has been found so far, and patients need life-long treatment in order to stabilize thei...

AI Technical Summary

Problems solved by technology

At present, a large number of studies have shown that non-coding RNA is involved in the pathogenesis of diabetes in various stages of islet development, islet cell apoptosis, insulin production, secretion, and insulin action, but the research methods are mostly limited to animal models or diseased tissues. and require traumatic means to obtain

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