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Kaikoujian extract, Its preparing method and use

A kind of open arrow, extract technology, applied in the direction of drug combination, pharmaceutical formula, plant raw materials, etc., can solve the problem of undetermined active ingredient compound properties of open arrow

Inactive Publication Date: 2006-05-24
SOUTHERN MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These studies are all based on the crude methanol extract of Open Arrow, and the compound properties of the active ingredients of Open Arrow have not been determined.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] Add 0.2 kg of open arrow rhizome slices (2-4mm), add 1.5 liters of water, soak for 6 hours, heat and cook for 2 hours, pour out the decoction; then add 1.5 liters of water, continue to decoct for 1.5 hours, pour out the decoction, and add water 1.5 liters, decoct in the same way for 1.5 hours; combine 3 decoctions, filter, and set aside.

[0058] HPD-100 macroporous resin column chromatography: HPD-100 macroporous resin column bed volume is 4.5cm (inner diameter) × 58cm (height), about 920mL. The decoction of the above-mentioned open arrow was loaded into the sample, and eluted with distilled water at a flow rate of about 1.8 liters / hour. After washing with about 5 liters of distilled water, the effluent was detected by the sulfuric acid-phenol method to detect the polysaccharide reaction, which was light yellow; then eluted with 2.5 liters of 20% ethanol, and then eluted with 3 liters of 70% ethanol. The parts eluted with 70% ethanol were collected, and the detection ...

Embodiment 2

[0069] 0.2 kilograms of open arrow rhizome slices (2~4mm), use 1.5 liters of 95% ethanol, 75% ethanol and 50% ethanol successively, heat and reflux for extraction for 2 hours, combine the extracts, reclaim ethanol by distillation under reduced pressure, and obtain the soaked Add 400 ml of distilled water to dissolve the ointment, put it in a separatory funnel, add 200 ml of petroleum ether to extract 3 times, vibrate for 5 minutes each time, and let stand for 0.5 hour; the lower aqueous solution is extracted 4 times with 200 ml of water-saturated n-butanol, Shake for 5 minutes and let stand for 1 hour. Combine the n-butanol extraction solutions, recover n-butanol by distillation under reduced pressure, dissolve the residue with 50 ml of methanol, add 500 ml of acetone, let it stand overnight, pour out the upper layer solution carefully, and the precipitate is detected by Liebermann-Burchard reaction and Molish reaction. Positive for saponins. The precipitate was vacuum-dried ...

Embodiment 3

[0078] Add 0.2 kg of open arrow rhizome slices (2-4 mm), add 1.5 liters of methanol, heat and reflux for extraction for 2 hours, repeat the extraction 4 times, combine the extracts, distill under reduced pressure to recover methanol, add 400 ml of distilled water to dissolve the obtained extract, and place In the separatory funnel, add 200 ml of petroleum ether to extract 3 times, shake for 5 minutes each time, and let it stand for 0.5 hours; extract the lower aqueous solution with 200 ml of water-saturated n-butanol for 4 times, shake for 5 minutes each time, let stand for 1 hour . The n-butanol extraction solutions were combined, and the n-butanol was recovered by distillation under reduced pressure. The residue was tested to be positive by Liebermann-Burchard reaction and Molish reaction, and it was a saponin substance, that is, total saponins were obtained, weighing 25.4g. Take 12g of total saponins and dissolve in methanol, stir evenly with H-type thin-layer chromatograph...

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PUM

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Abstract

The present invention relates to a Chinese medicine tupistra chinensis extract, its preparation method and application. Said extract is obtained by extracting root, stem and leaf of tupistra chinensis, and is a brownish yellow powder. The characteristics of its chemical identification are as follows: Liebermann-Burchard reaction and Molish reaction are positive; the foam test is positive reaction, the foam height of alkaline liquor tube is higher than that of acid liquor tube; Rosen-Heimer reaction at 60deg.C can produce violet red color reaction, it is a steroid saponin compound. Said extract has the action of resisting tumor, resisting inflammation, inhibiting platelet agglutination and resisting thrombosis.

Description

field of invention [0001] The invention relates to the field of traditional Chinese medicine, in particular to a traditional Chinese medicine extract and its preparation method and application, in particular to the open arrow extract and its preparation method and application. Background technique [0002] Plants of the genus Tupistra belong to the Convallarieae family of Liliaceae. There are more than 20 kinds of Arrow plants in total. my country is the center of the distribution of Arrow plants in the world, and the species account for about 70% of the world's total. Open arrow is a plant genus with a high content of endemic to my country, mainly distributed in southern regions such as Sichuan, Hubei, Yunnan, Zhejiang, Guangxi, Jiangxi, and Fujian. [0003] Arrowhead is a folk medicine in my country, and its rhizomes and whole plants are mostly used as medicine. The traditional theory of traditional Chinese medicine believes that this genus of plants is bitter, pungent, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/8967A61P7/02A61P29/00A61P35/00
Inventor 朱正光余传林雷林生吴曙光
Owner SOUTHERN MEDICAL UNIVERSITY
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