150results about How to "Good separation and purification effect" patented technology

The invention relates to a method for extracting high-purity tea saponin from an oil-tea-cake by decompression boiling. In the method, a degreased oil-tea-cake is used as a raw material, hydrous ethanol is used as an extraction solvent, and decompression boiling is adopted for extraction; after being filtered, extract passes through a ceramic membrane; ethanol is recovered from the tea saponin solution passing through the ceramic membrane, and the tea saponin solution is concentrated; and the concentrated solution is adsorbed through macroporous resin and chromatographically separated by medium and low pressure columns to prepare the high-purity tea saponin. The tea saponin prepared by adopting the technology has light color and high purity of over 95 percent, and the method becomes a novel technology for producing tea saponin product in the industry.

The invention provides a hepatic carcinoma targeted photo-thermal therapeutic agent as well as a preparation method and an application thereof and belongs to the field of biomedical engineering. The hepatic carcinoma targeted photo-thermal therapeutic agent is prepared with the method comprising the following steps: (1) preparing biotinylation nanometer microbubbles; (2) preparing a Cy7 fluorescently-labeled biotinylation anti-GPC3 (glypican3) antibody; (3) preparing biotinylation RGO (reduced graphene oxide); (4) coupling the products prepared in the step (1), step (2) and step (3) through a biotin-avidin system to obtain the hepatic carcinoma targeted photo-thermal therapeutic agent. The hepatic carcinoma targeted photo-thermal therapeutic agent has ultrasonic and fluorescence imaging functions, mediates RGO targeted delivery with an ultrasound-targeted microbubble destruction technology, monitors a photo-thermal therapeutic process in real time, can be used for preparing drugs for hepatic carcinoma therapy and has excellent clinical application value.

The invention relates to a Brasenia schreberi polysaccharide preparation method. The preparation method comprises the following steps: drying a raw material fresh Brasenia schreberi, and crushing to obtain Brasenia schreberi powder; adding water to the Brasenia schreberi powder for dilution, adjusting the pH value of the obtained solution, adding papain, carrying out enzymatic hydrolysis treatment under controlled conditions, filtering, concentrating, adding a mixture containing organic solvents comprising chloroform and n-butanol, and stirring for removing proteins; carrying out dialysis desalting through a dialysis membrane, carrying out alcohol precipitation, collecting the obtained precipitate, washing, and drying to obtain crude Brasenia schreberi polysaccharides. The preparation method has a high protein removal rate, a small polysaccharide loss and a good separation and purification effect, and determination results show that the protein removal rate reaches above 80%, and the purity of the obtained crude Brasenia schreberi polysaccharides reaches above 70%. The preparation method has the advantages of reasonable technology, advanced technique, feasible operation, and suitableness for the large-scale factory production.

The invention discloses a method for improving purity of eicosapentaenoic acid ethyl ester in fish oil. The method comprises steps as follows: the fish oil is subjected to transesterification, and a fatty acid ethyl esterification product is obtained; the fatty acid ethyl esterification product is subjected to urea inclusion, and a urea inclusion mixture is obtained; the urea inclusion mixture is subjected to at least three stages of molecular distillation, and a molecular distillation product is obtained; the molecular distillation product is subjected to rectification. According to the method, the technology is simple, the content of eicosapentaenoic acid ethyl ester in the fish oil can be effectively increased, meanwhile, the content of docosahexaenoic acid ethyl ester is reduced, and the separation effect is good, so that eicosapentaenoic acid ethyl ester can be better applied to industrial production.

The invention relates to a method for efficiently separating and purifying multiresidue sulfonamide antibiotics in a biological sample. The method is characterized by comprising the following steps of: (1) preparing a molecular imprinted polymer, comprising the steps of burdening according to the mol ratio of template molecule to functional monomer to cross linking agent of being 1: (2-8): 25 and obtaining the molecular imprinted polymer with special identification function to the sulfonamide antibiotics by adopting a body polymerization method; (2) weighing certain amount of biological sample to be separated into a mortar, grinding the biological sample by the mortar, adding right amount of the molecular imprinted polymer, and further homogenating to obtain a homogenated sample, wherein the weight ratio of the biological sample to be separated to the molecular imprinted polymer is 2: (1-5), and (3) placing the homogenated sample in a solid phase extraction column, sequentially rinsing with 2.0-4.0ml of water, 2.0-4.0ml of 10wt% acetonitrile aqueous solution, and then eluting with 3.0-5.0ml of methanol/methanoic acid mixed solution; and separating the sulfonamide antibiotics from the biological sample to be separated by being instantly dissolved in the methanol/methanoic acid mixed solution. The method provided by the invention has wide selectivity and good separation and purification effect.

The present invention separates and purifies taxol by means of column chromatographic process with macroporous resin, such as AB-8, D101, D201 and AK-9 as fixed phase and polar solvent, such as water solution of ethanol or acetone, as flowing phase, with the effluent flow rate is 0.6-1.5 L/hr. The effluent with taxol content lower than cephalotmannine content is concentrated and dried to obtain intermediate taxol product A; and effluent with taxol content higher than cephalotmannine content is concentrated and dried to obtain intermediate taxol product B. The said process can separate and purify coarse taxol product with 9-20% content to obtain intermediate taxol product with 40-60% content in the yield as high as 80%. The process can eliminate water soluble impurity from the coarse product.

The invention discloses a chromatographic purification method of fatty acid mono-acylation insulin. The chromatographic purification method comprises the steps of separating the fatty acid mono-acylation insulin by employing a medium- and low-pressure chromatographic system, and using an organic polymer reversion phase chromatographic material as a stationary phase, and taking at least one organic solvent mixable with water and at least one buffering agent substance as an elution moving phase with the pH (Potential of Hydrogen) value of 2-4. The chromatographic purification method has simple steps, is easy to amplify, and low in economic cost, and can increase the purity of a sample from original 50-60% to 98% by one step.

The invention discloses a method for microwave-assisted reverse micelle separation and purification of kidney bean agglutinin, and relates to a separation and purification method of the agglutinin, aiming at solving the problems of low ratio, high cost, more processes and long time for separation and purification of the kidney bean agglutinin. According to the method for the microwave-assisted reverse micelle separation and purification of the kidney bean agglutinin, the agglutinin is directly separated and purified from kidney bean agglutinin water extract; and the method comprises a pre-extraction process and a back extraction process. Under an auxiliary effect of microwave, water-soluble protein in kidney bean is quickly dissolved into the water extract, so that the dissolubility is greatly improved, and the leaching time is shortened. Based on a liquid-liquid extraction principle, the selectivity of extraction is improved, and high-efficiency pre-extraction and back extraction of the kidney bean agglutinin can be realized. By applying a reverse micelle extraction technology, a better separation and purification effect can be achieved with only the pre-extraction and back extraction steps; the method has simple steps and is convenient to operate; and the pre-treatment time can be greatly shortened in combination with microwave assistance so as to save the cost.

The invention discloses a production technology for extracting usnic acid from usnea longissima. The technology comprises the steps that a raw material is subjected to coarse grinding, and fed in from a feeding hopper; an extraction tank unit host rotates to slowly push the material from the front end of a unit backwards; an extraction solvent enters an extraction tank from a liquid inlet tube at the tail end of the unit, passes through the moving material from the back end of the tank, and flows towards the front end; and solid and liquid substances are contacted fully in an inverse motion, so that effective components in the medicinal material are extracted. Dregs are forcibly pushed to a dreg discharge port by a discharge transport and then discharged; a special juice squeezer squeezes the dregs; residual medicine liquor in the dregs is squeezed out of a medicinal material tissue; and the content of the residual medicine liquor in the dregs is reduced. The extraction efficiency is higher, and the extraction is more complete. The method adopts a continuous extraction tank unit for extraction, and is combined with a silica gel column chromatography for purification, so that a separating effect is better. Finished usnic acid with a maximum yield of 1% and the content above 95% can be obtained.

The invention discloses a preparation method of drug-resistant pseudomonas aeruginosa broad-host range phage, which relates to a preparation method of phage and solves the problem of poor separation and purification effect in the traditional separation method of the pseudomonas aeruginosa phage. The method comprises the following steps: 1, selecting a sewage sample; 2, preparing a lysis solution; 3, carrying out mixed cultivation on the lysis solution and a pseudomonas aeruginosa host cell suspension to obtain a mixed solution; 4, preparing a single phage plaque; and 5, inoculating the phage into a pseudomonas aeruginosa-containing culture medium, culturing until the pseudomonas aeruginosa is completely dissolved, and finally filtering. The preparation method of pseudomonas aeruginosa phage has the advantages of simple operation, good separation and purification effect and no phage breakage. The prepared drug-resistant pseudomonas aeruginosa broad-host range phage can be used for manufacturing autoimmune (vaccines) biological agents for treating diseases caused by the infection of drug-resistant pseudomonas aeruginosa, can be especially used as a topical spray for treating epidermal infectious diseases, and can avoid the drug-resistant pseudomonas aeruginosa produced by the use of antibiotics.

The invention provides a continuous chromatographic separation and purification system for separating sugar in inulin. A plurality of separation units which are connected together in series are driven by a turntable to rotate, and when the turntable rotates a circle, under the action of cation exchange resin filled in the separation units, fructo-oligosaccharide flows out of an elution area, polyfructose flows out of a separation area, and recycled water flows out of a concentration area. In such a manner, an inulin solution is separated and purified, so that links of exchange, washing, recycling, leaching and the like in an original fixed bed are integrated in the same system to achieve a better separation and purification effect.

The invention provides a method for preparing Bacilli Calmette-Gurin CpG-DNA (BCG-CpG-DNA) by utilizing Q Sepharose HP ion exchange. The method comprises the following steps: carrying out culture breeding of BCG, cracking to obtain a BCG lysate, centrifuging the lysate, directly removing impurities comprising polysaccharides, proteins, RNA and the like from the centrifuged BCG lysate to obtain purified BCG-CpG-DNA. There is no organic solvent residual in the BCG-CpG-DNA prepared through the method.

The invention discloses a fritillariae cirrhosae bulbus polysaccharide extraction, separation and purification technology. As a novel supercritical CO2 extraction technology is utilized, the defects that a general method is low in extraction rate and not high in purity are overcome; quaternary ammonium salt cetyl pyridinium chloride monohydrate and fritillariae cirrhosae bulbus polysaccharide can precipitate in water solution with low ionic strength; when the ionic strength is high, precipitate can be dissolved, dissociated and released, and the purification effect is good; DEAE-Sephadex ionic exchange column chromatography is utilized to further remove neutral impurities and impurities with positive charges, and the separation and purification effect is better.

The invention discloses a preparation method of high-purity anthocyanins by extracting from roselle, and relates to the extraction method of anthocyanins. The preparation method successively comprises the following specific steps: raw material pretreatment, extraction, enzymatic hydrolysis, centrifugalization, ultrafiltration, nanofiltration, concentration, microwave drying and crushing. The preparation method has the beneficial effects that the purity of an anthocyanin extract of the roselle can be improved from 2 to 3% to 30% or above, the energy can be saved, the process is simple, the cost is low, and the mass production can be facilitated.

The invention discloses a method for separating chenodeoxycholic acid from ursodesoxycholic acid, which belongs to the technical field of biochemical separation and is characterized by comprising the following steps in turn: (1) preparing column loading solution, namely dissolving mixture of ursodesoxycholic acid and chenodeoxycholic acid in alkaline aqueous solution according an equivalence ratio of 1:2.5, regulating the pH value of the solution to 7 to 8, and diluting deionized water till the solid content is 10 to 20 percent; (2) loading onto a column for absorption, namely pumping the column loading solution into a resin column filled with nonpolar porous synthetic absorbent; (3) desorbing, namely preparing eluting solution by using water soluble organic solvent, and pumping the eluting solution into the resin column for desorbing; and (4) performing post-treatment, namely concentrating under vacuum, recovering solvent, adding acid solution into the concentrated product to regulate the pH value to 2 to 3, precipitating, filtering, washing till the pH value is 7, drying, crystallizing and drying under vacuum to obtain a finished product. The method has the advantages that: the macroporous resin has high separation and purification performance and high accuracy; the ursodesoxycholic acid is separated from chenodeoxycholic acid with high yield; the process is simple; the production cost is low; and the industrial production efficiency is high.

The invention belongs to the chemical engineering field, and particularly discloses a method for preparing 3,4-dichloro bromobenzene. The method comprises steps of adding reduced iron powder into the raw material o-dichlorobenzene, inletting bromine water under certain pressure and temperature, and carrying out bromination reaction in a bromination kettle; carrying out multistage crystallization on the crude bromized 3,4-dichloro bromobenzene through solution, so as to purify the 3,4-dichloro bromobenzene, wherein the crude bromized 3,4-dichloro bromobenzene is crystallized by at least one stage, generally by multiple stages, namely, from one stage to three stages, so that a complete cycle of crystallization and purification of the 3,4-dichloro bromobenzene product is formed. The method has the main beneficial effects that the product is not polluted by foreign solvents or other materials, for the byproduct 2,3- dichloro bromobenzene generated in preparation of 3,4-dichloro bromobenzene through o-dichlorobenzene is used as the solvent; particularly, reduction of the yield of 3,4-dichloro bromobenzene caused by intensified side reaction of the 2,3- dichloro bromobenzene due to rectification high temperature is avoided; the method has the characteristics of high yield of the product, high purity and high production efficiency.

The invention relates to the technical field of biomaterials, in particular to a preparation method of aromatic extracts from field muskmelon. The method comprises the steps as follows: firstly, fruitfield muskmelons are crushed, ground and then dissolved with absolute ethyl alcohol, an ultrasonic dispersion device is used for accelerating dissolution of effective substances, then, a dispersion liquid is subjected to reflux and reduced pressure evaporation with petroleum ether, a crude extract is obtained and subjected to full extraction with ethyl acetate, and an extract A and a residual extract B are obtained; a class-2 aromatic extract rich in ester aromatic substances can be obtained from the extract A through freeze drying, and a class-1 aromatic extract containing furans, aldehydesand sulfur-containing compounds can be obtained from the residual extract B through negative-pressure adsorptive filtration and rectification treatment. By the aid of the method, classified extractionof various aromatic substances in the field muskmelons can be realized, the extraction rate of effective substances is high, and the product purity is high after classified purification.

The invention relates to a process and a system for producing a cyclohexane plasticizer by liquid phase hydrogenation of an o-benzene plasticizer. The process for preparing a cyclohexane plasticizer by liquid phase hydrogenation of an o-benzene plasticizer comprises the following steps: (1) heated inert gas is introduced into the bottom of a reduced pressure distillation tower in a pulse pneumaticconveying manner; and (2) a plasticizer product is preheated and then enters the bottom of the reduced pressure distillation tower, and the inlet of the plasticizer product on the reduced pressure distillation tower is higher than the inlet of the inert gas, so that the plasticizer product is mixed and heated with the rising inert gas flow in the downward flowing process, and the target product is separated from the by-product. According to the process and the system disclosed by the invention, heated inert gas is fed into the reduced pressure distillation tower in a pulse manner, and the characteristic that the flow of inert gas is changing is utilized to better simulate the characteristics of fluidization, mixing and heating at the bottom of the reduced pressure distillation tower, so that better mixing, heating, separation and purification effects on the plasticizer product are realized.