Novel production process for total flavones of hippophae rhamnoides

Abstract

The invention discloses a novel production process for total flavones of hippophae rhamnoides. The process comprises the steps as follows: washing raw materials; adding into a feeding hopper; rotating a mainframe of an extraction tank set; enabling materials to be fed into the front end of the extraction tank set backwardly and slowly; at the same time, enabling extracting solvents to enter into extraction tanks from a liquid inlet pipe at the tail end of the extraction tank set, penetrate through the moving materials from the rear ends of the tanks and flow to the front ends; and enabling the solid phase materials and the liquid phase materials to be fully contacted in the inverse motion so as to extract effective ingredients from the medicinal materials. Dregs are forcedly conveyed to a discharge hole for discharge through a discharge conveyer and squeezed by a special squeezer to squeeze out residual liquor in the dregs from the tissues of the medicinal materials so as to reduce the content of the residual liquor in the dregs. The extraction efficiency is higher and the extraction process is safer. The macroporous adsorption resin and the silica column chromatography for purification are selected and used at the same time, so that the separation effect is better. The finished total flavones of hippophae rhamnoides with the highest yield of 2% and the content of more than 70% can be obtained.

Description

Technical field: [0001] The invention belongs to the field of natural organic chemistry, and relates to a production process of total flavonoids of seabuckthorn, in particular to a new production process of purifying total flavonoids of seabuckthorn by countercurrent extraction and macroporous adsorption resin coupling silica gel column chromatography. Background technique: [0002] Sea buckthorn Hippophae rhamnoides Linn, also known as vinegar willow, yellow sour thorn, sour thorn willow, black thorn, sour thorn, is the fruit of Hippophae rhamnoides Linn. Sea buckthorn fruit contains flavonoids isorhamnetin, isorhamnetin-3-O-β-D-glucoside, isorhamnetin -3-O-β-rutinoside (isorhamnetin-3-O-β-rutinoside), rutin (rutin), astragalin (astragalin) and quercetin (quercetin) and kaempferol (kaempferol) are Low glycosides of aglycones. Also contains vitamins (vitamin) A, B1, B2, C, E, dehydroascorbic acid (dehydroascorbic acid), folic acid (folic acid), carotene (carotene), caroten...

AI Technical Summary

Problems solved by technology

The extraction process of seabuckthorn total flavonoids in the above-mentioned patent documents is mainly to obtain the raw juice and pericarp after being squeezed. The flavonoids are obtained by degreasing, desolventizing, and drying the intermediates with non-ether solvents. Although the final total flavonoids of seabuckthorn are of high purity, the process itself is expensive, and the actual application effect is far inferior to that of countercurrent extraction and macroporous adsorption resins. Effect of Coupled Silica Column Chromatography Separation