Micro-fluidic chip for enriching and purifying CTCs (circulating tumor cells)

Abstract

The invention discloses a micro-fluidic chip. The micro-fluidic chip comprises positioning holes located in four corners of a chip body, a sample adding hole, a sample reaction cavity, a physical screening flow channel, a liquid waste flow channel, a liquid waste collecting cavity, a magnetic adsorption cavity plated with a magnet on the upper surface, a product flow channel, a product collectingcavity and a product absorption hole, wherein the sample reaction cavity and the magnetic adsorption cavity are communicated through the physical screening flow channel; the magnetic adsorption cavityand the product collecting cavity are communicated through the product flow channel; the lower side wall of the physical screening flow channel is communicated with the liquid waste flow channel through a notch formed in the lower side wall. The invention further discloses a method for enriching and purifying CTCs (circulating tumor cells) by adopting the micro-fluidic chip. An experiment can becompleted by only one centrifugal machine, the operation is fast and simple, an expensive EpCAM antibody is not needed, and the cost is relatively low; the obtained CTCs not only expresses the type ofthe EpCAM antibody, but also has better separation and purification effects, no immunomagnetic beads exist on the surface, and biological activity of the micro-fluidic chip is better.

Description

technical field [0001] The invention relates to the technical field of molecular biomedicine, in particular to a microfluidic chip for enriching and purifying circulating tumor cells. Background technique [0002] In recent years, the incidence of tumors is increasing year by year, and malignant tumors have become the leading cause of death among Chinese residents. One of the main characteristics of malignant tumors is metastasis, which means that tumor cells detach from the primary tumor after a large number of proliferations, and then invade blood vessels, lymphatic vessels or body cavities. Metastasis in a distant site or organ. Circulating Tumor Cells (CTCs) is a general term for all kinds of tumor cells that break away from the primary tumor site and metastatic site and enter the blood circulation. CTCs, as a bridge between primary tumors and metastases, carry a large amount of information about tumor progression and metastasis, and undergo epithelial-mesenchymal tran...

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Problems solved by technology

The method described in the Chinese patent with the application number CN201610344920.4 is similar to the method described in the Chinese patent with the application number CN201711446591.5. The structure of the microarray column used in this method is complex, and the production requirements for the microfluidic chip are very fine ,high cost

Moreover, this method lacks the further screening process of magnetic beads, and cannot separate larger white blood cells. It also needs to use an external pump to apply a fixed flow rate to the sample, which requires higher equipment requirements.

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