Method for preparing saikoside from bupleurum longiradiatum

Abstract

The invention discloses a method for preparing saikoside from bupleurum longiradiatum. The method comprises the steps that a raw material is subjected to coarse grinding, and fed in from a feeding hopper; an extraction tank unit host rotates to slowly push the material from the front end of a unit backwards; an extraction solvent enters an extraction tank from a liquid inlet tube at the tail end of the unit, passes through the moving material from the back end of the tank, and flows towards the front end; and solid and liquid substances are contacted fully in an inverse motion, so that effective components in the medicinal material are extracted. Dregs are forcibly pushed to a dreg discharge port by a discharge transport and then discharged; a special juice squeezer squeezes the dregs; residual liquor in the dregs is squeezed out of a medicinal material tissue; and the content of the residual liquor in the dregs is reduced. The extraction efficiency is higher, and the extraction is more complete. The method adopts a continuous extraction tank unit for extraction, and is combined with a silica gel column chromatography for purification, so that a separating effect is better. Finished saikoside with a maximum yield of 4% and the content above 95% can be obtained.

Description

Technical field: [0001] The invention belongs to the field of natural organic chemistry, and relates to a preparation method of saikosaponin, in particular to a method for preparing saikosaponin from Bupleurum bupleuri by continuous countercurrent extraction combined with solvent extraction. Background technique: [0002] Bupleurum longiradiarum, Latin name: Bupleurum longiradiarum., aliases: Cihu, Dixun, Shancai, Rucao, Firewood, perennial tall herb, 80-150 cm high. The rhizome is curved, firm, yellowish-brown, with many fibrous roots in dense links; the stem is solitary or 2-3, with thick grooves and many branches; the leaves are large, slightly sparse, bright green on the surface, with pink-blue-green on the back; petals Oblate, apex folded inwards, base of lamina broad, not protruding; fruit dark brown, covered with white powder, oblong-elliptic. Produced in Heilongjiang, Jilin, Liaoning, Inner Mongolia, Gansu and other provinces. [0003] Saikosaponin is a kind of tri...

AI Technical Summary

Problems solved by technology

The saikosaponin extraction process in the above-mentioned patent documents is mainly extracted through the steps of rolling extraction and macroporous resin purification. Although the final saikosaponin has a high purity, the process itself is expensive and the actual application effect is far inferior to that of Separation Effect of Macroporous Adsorbent Resin Coupled with Silica Gel Column Chromatography